Food & Function, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
EA enhanced the abundance of beneficial bacteria as well their derived metabolites, thereby inhibiting chronic alcohol-induced neuroinflammation via regulation gut microbiota-mediated CCL21-CCR7 axis, ultimately ameliorating AICSD.
Language: Английский
Frontiers in Neuroanatomy, Journal Year: 2025, Volume and Issue: 19
Published: March 7, 2025
Introduction A growing body of evidence suggests that the gut microbiome may contribute to changes in brain morphology. The microbiota-gut-brain axis (MGBA) has been shown influence neurogenesis, axon myelination, and synapse structure. However, it remains unclear whether MGBA can morphology density inhibitory GABAergic interneurons. aim this study was determine antibiotic-induced dysbiosis (AID) is associated with alterations dendritic interneurons medial entorhinal cortex (mEC), somatosensory (SSC), motor (MC), hippocampus (Hp). Methods cohort six-month-old GAD-67-EGFP transgenic mice treated an antibiotic cocktail for two weeks, resulting as validated by collecting stool samples at baseline after treatment, then using next-generation sequencing 16S ribosomal RNA. Results results demonstrate proposed model effectively exhibited defining features dysbiosis, including a significant reduction diversity, expansion pathobionts, loss beneficial microbes. AID group showed different areas. mean length segments SSC Hp were found be significantly decreased, while no such decrease observed mEC or MC. Furthermore, decreased mEC, Hp, areas, change MC area. Discussion interneuron dysfunction plays role pathogenesis neurological disease. findings suggest potentially influences interneurons, which development disorders.
Language: Английский
Citations
1
Pharmacological Research, Journal Year: 2025, Volume and Issue: 213, P. 107661 - 107661
Published: Feb. 20, 2025
Perturbed cholesterol metabolism may play an important role in the development of dementia and its preclinical stage, mild cognitive impairment (MCI). Oxysterols, metabolites generated during oxidation, also appear to be risk factors for MCI. Therefore, we aimed investigate if metabolic profile blood oxysterols could used characterize MCI risk. This cross-sectional study incorporated 501 participants-253 patients with 248 cognitively normal controls. Serum levels 22 free were measured, a set 27 oxysterol-related gene polymorphisms was genotyped. Five [27-hydroxycholesterol (27-OHC), 27-OHC periphery-derived metabolite 3β-hydroxy-5-cholestenoic acid (27-CA) brain-derived 7α-hydroxy-3-oxo-4-cholestenoic (7-HOCA), 4β-hydroxycholesterol (4β-OHC); 4α-hydroxycholesterol (4α-OHC)] twenty-two detected serum significantly differed between controls, greatly distinguishing from control individuals (AUC=0.834, 95% CI: 0.804-0.866). Association analyses demonstrated significant correlations these candidate oxysterol biomarkers younger age, higher lipids, worse performance, monounsaturated fatty intake. panel as highlighted essential pathogenesis early prevention. (The registered Chinese Clinical Trial Registry ChiCTR-OOC-17011882).
Language: Английский
Citations
0
Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 601 - 601
Published: Feb. 19, 2025
Tonic immobility (TI) serves as an indicator of innate stress response recovery in poultry. Broilers with different TI phenotypes exhibit varying levels aggressive behavior, which can significantly impact their welfare. However, the influences on broiler aggression remain largely unexplored. In this study, chickens were stratified into two distinct phenotypic groups based duration: short (STI) and long (LTI). The impacts investigated by analyzing cecal intestinal morphology, bacteria, plasma metabolites, corticosterone levels. Compared to LTI broilers, STI broilers showed reduced (CORT) (p < 0.05) a decreased frequency behaviors, including dominant subdominant types 0.01). Histological analysis revealed that have increased duodenal villus height villus-height-to-crypt-depth ratio 0.01), jejunal crypt depth ileal 0.01) compared broilers. 16S rDNA sequencing Linear discriminant effect size (LefSe) identified differential bacterial abundance, notably genus cc115 belonging Firmicutes. Specific microbiota exhibited significant positive correlations behavior corticosterone, while those negative correlations. Untargeted metabolomics 21 downregulated 17 upregulated metabolites between phenotypes. Correlation 10 positively correlated whereas 8 negatively correlated. higher content more intense than behavioral physiological profiles observed are strongly specific gut metabolite profiles. microbial signatures serve key biomarkers for regulating represent potential early indicators detecting issues poultry farming.
Language: Английский
Citations
0
Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)
Published: March 16, 2025
Roseburia intestinalis is one of the most abundant and important butyrate-producing human gut anaerobic bacteria that plays an role in maintaining health a potential next-generation probiotic. We investigated pangenome 16 distinct strains, isolated over several decades, identifying local time-specific adaptations. More than 50% genes each individual strain were assigned to core genome, 77% cloud unique revealing high level genome conservation. Co-carriage same enzymes involved carbohydrate binding degradation all strains highlighted major pathways utilization reveal importance xylan, starch mannose as key growth substrates. A single had adapted use rhamnose sole substrate, first time this has been reported. The ubiquitous presence motility sporulation gene clusters demonstrates these phenotypes for survival acquisition bacterium. half contained functional, potentially transferable, tetracycline resistance genes. This study advances our understanding R. within ecosystem by elucidating conserved metabolic characteristics among different from locations. information will help devise dietary strategies increase abundance species providing benefits.
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156758 - 156758
Published: April 1, 2025
Language: Английский
Citations
0
Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1029 - 1029
Published: April 29, 2025
The COVID-19 pandemic has highlighted the need for a comprehensive understanding of factors influencing disease severity and progression. Emerging research indicates that human microbiota, particularly beneficial bacteria, significantly impacts immune responses health outcomes in patients. While existing studies provide general insights into relationship between microbiota probiotics with COVID-19, they often lack detailed exploration how specific bacterial taxa might be used as adjunctive treatments. This review aims to address this gap by focusing on ten key genera discussing their roles evaluating potential prevention treatment. covers impact these microbes health, population alterations patients, interactions other viral infections. Among microbes, several exhibit distinct patterns abundance highlighting infection dynamics. In populations Akkermansia, Ruminococcus, Roseburia are consistently reduced, while those Faecalibacterium show significant decline more severe cases. Bacteroides presents varying effects depending species involved. Alterations Blautia Lachnospiraceae associated increased inflammation severity. Likewise, depletion Lachnospira Coprococcus populations, both linked anti-inflammatory effects, may exacerbate symptom Oscillospira, though less studied, is connected overall could have implications synthesizes current highlight importance maintaining healthy alleviate contribute development novel therapeutic strategies involving modulation.
Language: Английский
Citations
0
Alzheimer s Research & Therapy, Journal Year: 2025, Volume and Issue: 17(1)
Published: May 13, 2025
Oxysterols, gut metabolites, and N6-methyladenosine (m6A) are extensively implicated in the pathogenesis of cognitive dysfunction, while their alterations different stages mild impairment (MCI) have not been elucidated. Therefore, this study was conducted to explore associations oxysterols, m6A methylation profiles early MCI (EMCI) late (LMCI) individuals. Liquid chromatography-mass spectrometry, untargeted metabolomic analysis, mRNA Epitranscriptomic Microarray were used detect characteristics serum oxysterols (n = 35/group), fecal metabolites 30/group), whole blood 4/group) respectively. The concentration β-amyloid (Aβ) detected with ELISA 25/group). gene expression amyloid precursor protein (APP) its key enzyme β-secretase (BACE1) measured by quantitative real-time PCR EMCIs LMCIs, especially exhibited poorer performance almost all global multidimensional tests. Serum 27-hydroxycholesterol (27-OHC) 24S-hydroxycholesterol (24S-OHC) elevated EMCI LMCI groups. Changes occurred mainly group, which several including Procyanidin dimer B7 Phorbol myristate, significantly decreased. landscape LMCIs obviously differed from Controls. Hypomethylated mRNAs accounted for majority accompanied downregulated mRNAs, consistent writer methyltransferase-like 4 (METTL4). 27-OHC 24S-OHC combined various distinguished between subgroups healthy controls (EMCI/Control: AUC 0.877; LMCI/Control: 0.952). Heatmap revealed correlation myristate differentially m6A-methylated mRNAs. Differentially expressed methylated commonly enriched 34 KEGG metabolic pathways, cholesterol metabolism neurodegenerative disease-related pathways. Our explored altered MCI. potential function aberrant driving progression warrants further mechanistic investigation.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: July 13, 2024
Abstract The Apple polysaccharides (AP), extracted from the fruit of apple, has been used to treat multiple pathological diseases. In this study, we evaluated effects AP on cognitive impairment and intestinal aging in naturally mice. As a result, it was found that could improve spatial learning memory mice through Morris water maze experiment. Additionally, intervention can upregulate expression nerve growth factor (BDNF), postsynaptic marker (PSD95), presynaptic (SYP) proteins. Moreover, enhance total antioxidant capacity, reduce level pro-inflammatory cytokine, inhibit activation NF-κB signaling pathway, exerting anti-inflammatory functions. And administration restored mucosal barrier function, reduced apoptosis related also altered gut microbiota At genus level, decreased abundance Helicobacter Bilophila , while increased Lactobacillus Bacteroides . summary, these data demonstrate treatment alleviate impairment, oxidative stress, inflammatory reactions, repair barrier, aging, alter specific microbial characteristics, ultimately improving health elderly.
Language: Английский
Citations
2
Pharmacological Research, Journal Year: 2024, Volume and Issue: 208, P. 107385 - 107385
Published: Sept. 6, 2024
Language: Английский
Citations
2
Food & Function, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
EA enhanced the abundance of beneficial bacteria as well their derived metabolites, thereby inhibiting chronic alcohol-induced neuroinflammation via regulation gut microbiota-mediated CCL21-CCR7 axis, ultimately ameliorating AICSD.
Language: Английский
Citations
1