Microplastic and the Enteric Nervous System: Effect of PET Microparticles on Selected Neurotransmitters and Cytokines in the Porcine Ileum DOI Open Access
Ismena Gałęcka, Jarosław Całka

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11645 - 11645

Published: Oct. 30, 2024

Microplastic is an environmental hazard to which both animals and humans are exposed. Current reports show that it can cause inflammation, including in the gastrointestinal tract. To examine impact on ileum, 15 eight-week-old gilts (five individuals/group) were exposed PET microplastics (7.6 µm-416.9 µm) at a dose of 0.1 g/day or 1 for 28 days. The collected ileum fragments investigated cytokine concentrations (IL-1β, IL-6, IL-8, IL-10, TNF-α; ELISA test), neuron populations (cocaine amphetamine-regulated transcript, galanin, neuronal nitric oxide synthase, substance P, vesicular acetylcholine transporter, vasoactive intestinal peptide; immunofluorescence staining), morphometric parameters (histological analysis). Under influence MP-PET, there was reduction CART- GAL-positive neurons submucosal plexuses nNOS-, VAChT-, VIP-positive all plexuses. In contrast, increase myenteric plexus SP-positive IL-1β, TNF-α did not undergo statistically significant changes under low high MP-PET. histological structure exclusively concerned thinning mucosa muscularis externa. results support thesis MP-PET neutral ileal cells.

Language: Английский

Unseen Toxins: Exploring the Human Health Consequences of Micro and Nanoplastics DOI Creative Commons

John G. Dennis,

Divya Arulraj,

Tapan Kumar Mistri

et al.

Toxicology Reports, Journal Year: 2025, Volume and Issue: 14, P. 101955 - 101955

Published: Feb. 15, 2025

Language: Английский

Citations

0
Oral exposure to PET microplastics alters the pancreatic transcriptome-implications for the pathogenesis of type 1 diabetes DOI Open Access
Karol Mierzejewski, Aleksandra Kurzyńska, Monika Golubska

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Abstract Type 1 diabetes is a chronic autoimmune disease, the incidence of which has been steadily increasing in recent years, particularly among adolescents. The disease results from combination genetic and environmental factors that lead to destruction insulin-producing beta cells pancreas. Recently, potential role microplastics pathogenesis various diseases gained attention. Therefore, aim this study was investigate impact PET on pancreas using immature pigs as model organism. global transcriptomic profile analyzed piglets treated with either low (0.1 g/day) or high dose (1 for 4 weeks RNA-Seq. analysis revealed dose-dependent effect gene expression. A affected expression one gene, while impacted 86 genes. differentially expressed genes, including immune cell markers, cytokines chemokines, may activate system way characteristic type diabetes. It suggests oral exposure be new risk factor development disease.

Language: Английский

Citations

2
PET microplastics increase the risk of insulin resistance and pancreatitis DOI Creative Commons
Karol Mierzejewski, Aleksandra Kurzyńska, Monika Golubska

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract Microplastics and their effects on the body have recently been of great concern. Today it is clear that they are not indifferent to human health, but full spectrum impact has yet fully described. Pancreatic diseases becoming increasingly common worldwide, etiology well understood. Worryingly, these diagnosed in children over last 20 years, which was previously considered unusual. The aim study therefore determine changes pancreas caused by PET microplastics young organisms. For this purpose, global metabolomic profile piglets treated with a low (0.1 g/day) or high dose (1 for 4 weeks determined UPLC-MS analysis. In addition, insulin levels various biochemical parameters blood were analyzed. showed affected physiological processes at both doses. We found increased tissue important metabolites such as glucose, γ-aminobutyric acid, lysophosphatidylcholine lysophosphatidylethanolamine. concentrations dose-dependently regulated lipase, cholesterol calcium levels. These results suggest increase risk resistance pancreatitis.

Language: Английский

Citations

1
Microplastic and the Enteric Nervous System: Effect of PET Microparticles on Selected Neurotransmitters and Cytokines in the Porcine Ileum DOI Open Access
Ismena Gałęcka, Jarosław Całka

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11645 - 11645

Published: Oct. 30, 2024

Microplastic is an environmental hazard to which both animals and humans are exposed. Current reports show that it can cause inflammation, including in the gastrointestinal tract. To examine impact on ileum, 15 eight-week-old gilts (five individuals/group) were exposed PET microplastics (7.6 µm-416.9 µm) at a dose of 0.1 g/day or 1 for 28 days. The collected ileum fragments investigated cytokine concentrations (IL-1β, IL-6, IL-8, IL-10, TNF-α; ELISA test), neuron populations (cocaine amphetamine-regulated transcript, galanin, neuronal nitric oxide synthase, substance P, vesicular acetylcholine transporter, vasoactive intestinal peptide; immunofluorescence staining), morphometric parameters (histological analysis). Under influence MP-PET, there was reduction CART- GAL-positive neurons submucosal plexuses nNOS-, VAChT-, VIP-positive all plexuses. In contrast, increase myenteric plexus SP-positive IL-1β, TNF-α did not undergo statistically significant changes under low high MP-PET. histological structure exclusively concerned thinning mucosa muscularis externa. results support thesis MP-PET neutral ileal cells.

Language: Английский

Citations

0