Impact of eggshell membrane on metabolism and cell adhesion in oxidatively stressed canine chondrocytes DOI Creative Commons

J Vozár,

Nikola Hudáková, Natália Nosálová

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 8, 2025

Eggshell membrane (ESM) is a rich source of bioactive compounds, including proteins, peptides, and antioxidants, contributing to its potential therapeutic benefits. These natural antioxidants might help neutralize reactive oxygen species (ROS) modulate inflammatory responses, which are often linked with chondrocyte damage in osteoarthritis. In this study, we investigated the functional effects ESM proteins on H2O2-induced oxidative stress neonatal canine chondrocytes. The isolated chondrocytes demonstrated high proliferation rate increased glycosaminoglycan (GAG) production during cultivation. addition, expression key cartilage markers, collagen types II IX, aggrecan, confirmed retention phenotype. Under vitro conditions, post-treatment improved viability, indicating that may have reparative role mitigating damage. This significant was validated through XTT assays, measured cell metabolic activity at 24 h, Real-time Cell Analysis (RTCA), providing continuous monitoring over 98 h. contrast, preventive against were observed exclusively analysis. By investigating these aspects, provide insight into protect from damage, particularly repair joint health. study one first create vital platform based for dietary supplements designed prevent or dog Thus, offers valuable contribution understanding how compounds can be used therapeutically, bridging gap between findings practical applications veterinary medicine.

Language: Английский

Impact of eggshell membrane on metabolism and cell adhesion in oxidatively stressed canine chondrocytes DOI Creative Commons

J Vozár,

Nikola Hudáková, Natália Nosálová

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 8, 2025

Eggshell membrane (ESM) is a rich source of bioactive compounds, including proteins, peptides, and antioxidants, contributing to its potential therapeutic benefits. These natural antioxidants might help neutralize reactive oxygen species (ROS) modulate inflammatory responses, which are often linked with chondrocyte damage in osteoarthritis. In this study, we investigated the functional effects ESM proteins on H2O2-induced oxidative stress neonatal canine chondrocytes. The isolated chondrocytes demonstrated high proliferation rate increased glycosaminoglycan (GAG) production during cultivation. addition, expression key cartilage markers, collagen types II IX, aggrecan, confirmed retention phenotype. Under vitro conditions, post-treatment improved viability, indicating that may have reparative role mitigating damage. This significant was validated through XTT assays, measured cell metabolic activity at 24 h, Real-time Cell Analysis (RTCA), providing continuous monitoring over 98 h. contrast, preventive against were observed exclusively analysis. By investigating these aspects, provide insight into protect from damage, particularly repair joint health. study one first create vital platform based for dietary supplements designed prevent or dog Thus, offers valuable contribution understanding how compounds can be used therapeutically, bridging gap between findings practical applications veterinary medicine.

Language: Английский

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