MOMAST® Downregulates AQP3 Expression and Function in Human Colon Cells DOI Creative Commons

Ines Angelini,

Mariangela Centrone, Giusy Rita Caponio

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 14(1), P. 26 - 26

Published: Dec. 28, 2024

The water channel AQP3 is an aquaglyceroporin expressed in villus epithelial cells, and it plays a role transport across human colonic surface cells. Beyond water, can mediate glycerol H2O2 transport. Abnormal expression function of have been found various diseases often characterized by altered cell growth proliferation. Here, the beneficial effects MOMAST® evaluated. antioxidant-patented natural phenolic complex obtained from olive wastewater (OWW) Coratina cultivar. Treatment colon HCT8 cells with reduced viability. Confocal studies Western Blotting analysis demonstrated that treatment significantly decreased staining AQP3. Importantly, functional revealed reduction abundance correlates significant decrease uptake. Indeed, was partially but presence or DFP00173, selective inhibitor In addition, MOMAST®-induced associated epithelial-mesenchymal transition (EMT)-related proteins such as vimentin β-catenin. Together, these findings propose potential adjuvant abnormal targeting

Language: Английский

Herbal Extracts Mixed with Essential Oils: A Network Approach for Gastric and Intestinal Motility Disorders DOI Open Access
Roberta Budriesi, Ivan Corazza,

Simone Roncioni

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(24), P. 4357 - 4357

Published: Dec. 17, 2024

Background: Three herbal extracts (Asparagus racemosus Willd., Tabebuia avellanedae Lorentz, and Glycyrrhiza glabra L.) were mixed with three essential oils (Foeniculum vulgare Mill., Mentha piperita L., Pimpinella anisum to formulate a product (HEMEO) whose active compounds include saponins steroids in Asparagus racemosus, known for their anti-inflammatory properties; glycyrrhizin flavonoids glabra, which exhibit gastroprotective antispasmodic effects; menthol piperita, contributing antimicrobial anethole polyphenols anisum, modulate intestinal motility offer activity. Objective: HEMEO was formulated applications disorders. Methods: evaluated spontaneous induced effects isolated guinea pig ileum, colon, stomach. Ex vivo experiments conducted using LabChart software v7.0, the product’s antibacterial action against Helicobacter pylori its antioxidant assessed through disc diffusion FRAP assays. The presence of volatile formulation confirmed by GC-MS analysis; TPC HEMEO, determined Folin–Ciocalteu method, 9.925 ± 0.42 mg GAE/g. Conclusions: showed phenolic content correlated potential addition inhibited H. growth demonstrated notable properties, suggesting role as supportive agent digestive processes managing

Language: Английский

Citations

0
MOMAST® Downregulates AQP3 Expression and Function in Human Colon Cells DOI Creative Commons

Ines Angelini,

Mariangela Centrone, Giusy Rita Caponio

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 14(1), P. 26 - 26

Published: Dec. 28, 2024

The water channel AQP3 is an aquaglyceroporin expressed in villus epithelial cells, and it plays a role transport across human colonic surface cells. Beyond water, can mediate glycerol H2O2 transport. Abnormal expression function of have been found various diseases often characterized by altered cell growth proliferation. Here, the beneficial effects MOMAST® evaluated. antioxidant-patented natural phenolic complex obtained from olive wastewater (OWW) Coratina cultivar. Treatment colon HCT8 cells with reduced viability. Confocal studies Western Blotting analysis demonstrated that treatment significantly decreased staining AQP3. Importantly, functional revealed reduction abundance correlates significant decrease uptake. Indeed, was partially but presence or DFP00173, selective inhibitor In addition, MOMAST®-induced associated epithelial-mesenchymal transition (EMT)-related proteins such as vimentin β-catenin. Together, these findings propose potential adjuvant abnormal targeting

Language: Английский

Citations

0