Whole Genome Analysis of Pediococcus acidilactici XJ-24 and Its Role in Preventing Listeria monocytogenes ATCC® 19115TM Infection in C57BL/6 Mice DOI Creative Commons

Weizhong Hu,

Shuxin Zhou,

Amel Ibrahim

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 323 - 323

Published: March 19, 2025

Background/Objectives: As probiotics gain prominence in the prevention and treatment of intestinal diseases, their protective effects against pathogens influence on host health have drawn significant attention. This study investigates genomic characteristics functional potential Pediococcus acidilactici XJ-24 (XJ-24) Listeria monocytogenes (LM) infection mice. Methods/Results: Whole-genome analysis confirmed safety probiotic properties XJ-24, including acid bile salt tolerance, antimicrobial activity, safety. In vivo, C57BL/6 mice challenges indicated that significantly reduced LM colonization, suppressed pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-γ), alleviated colon spleen tissue damage, maintained barrier integrity by upregulating tight junction proteins (Occludin, Claudin-1, ZO-1). Moreover, modulated gut microbiota composition increasing beneficial taxa while reducing harmful bacteria. Correlation highlighted a positive association between Lachnospiraceae proteins. Conclusions: These findings demonstrate as for preventing provide basis further clinical exploration.

Language: Английский

Whole Genome Analysis of Pediococcus acidilactici XJ-24 and Its Role in Preventing Listeria monocytogenes ATCC® 19115TM Infection in C57BL/6 Mice DOI Creative Commons

Weizhong Hu,

Shuxin Zhou,

Amel Ibrahim

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 323 - 323

Published: March 19, 2025

Background/Objectives: As probiotics gain prominence in the prevention and treatment of intestinal diseases, their protective effects against pathogens influence on host health have drawn significant attention. This study investigates genomic characteristics functional potential Pediococcus acidilactici XJ-24 (XJ-24) Listeria monocytogenes (LM) infection mice. Methods/Results: Whole-genome analysis confirmed safety probiotic properties XJ-24, including acid bile salt tolerance, antimicrobial activity, safety. In vivo, C57BL/6 mice challenges indicated that significantly reduced LM colonization, suppressed pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-γ), alleviated colon spleen tissue damage, maintained barrier integrity by upregulating tight junction proteins (Occludin, Claudin-1, ZO-1). Moreover, modulated gut microbiota composition increasing beneficial taxa while reducing harmful bacteria. Correlation highlighted a positive association between Lachnospiraceae proteins. Conclusions: These findings demonstrate as for preventing provide basis further clinical exploration.

Language: Английский

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