Animal Model Screening for Hyperlipidemic ICR Mice DOI Open Access
Xingtong Chen,

Yunyue Zhou,

Jinbiao Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2142 - 2142

Published: Feb. 27, 2025

This study aimed to establish a hyperlipidemia model in ICR mice using homemade high-fat diet. It further investigated hyperlipidemia-related indicators control and at various feeding durations determine the optimal time frame for successful establishment. Sixteen male were introduced intervals of 3 weeks, starting from weeks 0, 3, 6, 9, 12. The group was fed standard diet, while received diet induce hyperlipidemia. Blood lipid related indices detected 15 weeks. liver, scapular fat, abdominal epididymal fat harvested calculate organ index. contents T-CHO, TG, TBA liver measured. HE staining used observe pathological changes tissue white adipose tissue, Oil Red O droplets tissue. mRNA protein expression SREBP-2, insig1, HMGCR, LXRα, ABCA1, CYP7A1 by RT-qPCR Western Blot. In group, blood levels significantly increased 9th week, aligning with indicative Insig-1, markedly elevated 9 remained relatively stable thereafter. provides reliable reference determining establishment models vivo animal experiments.

Language: Английский

Animal Model Screening for Hyperlipidemic ICR Mice DOI Open Access
Xingtong Chen,

Yunyue Zhou,

Jinbiao Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2142 - 2142

Published: Feb. 27, 2025

This study aimed to establish a hyperlipidemia model in ICR mice using homemade high-fat diet. It further investigated hyperlipidemia-related indicators control and at various feeding durations determine the optimal time frame for successful establishment. Sixteen male were introduced intervals of 3 weeks, starting from weeks 0, 3, 6, 9, 12. The group was fed standard diet, while received diet induce hyperlipidemia. Blood lipid related indices detected 15 weeks. liver, scapular fat, abdominal epididymal fat harvested calculate organ index. contents T-CHO, TG, TBA liver measured. HE staining used observe pathological changes tissue white adipose tissue, Oil Red O droplets tissue. mRNA protein expression SREBP-2, insig1, HMGCR, LXRα, ABCA1, CYP7A1 by RT-qPCR Western Blot. In group, blood levels significantly increased 9th week, aligning with indicative Insig-1, markedly elevated 9 remained relatively stable thereafter. provides reliable reference determining establishment models vivo animal experiments.

Language: Английский

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