Simultaneous Quantification of Caffeic and Ferulic Acids by HPLC‐UV in Nanoparticles: Degradation Profiling and Computational Peroxidation Analysis

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(4)

Published: March 6, 2025

Phenolic compounds such as ferulic acid (FA) and caffeic (CA) are renowned for their antioxidant anti-inflammatory properties. However, physicochemical instability limits broader therapeutic applications. This study aimed to develop an analytical methodology the simultaneous quantification of these in nanoparticles using liquid chromatography. The suspensions exhibited favorable characteristics, including a particle size 117 nm, encapsulation efficiency above 60%, zeta potential 14 mV, adequate bioactive content. Notably, system enhanced photostability under UV radiation. HPLC-UV method employed C18 column with mobile phase acidic water (pH 2.6), methanol, acetonitrile (64:18:18, v/v/v) at 1 mL/min, detection 223 achieving fast efficient separation. was linear, specific, precise, accurate, robust, accordance Brazilian ICH guidelines. Additionally, stability-indicating, identifying isomeric degradation products, which represent most common reaction compounds. Computational analysis density functional theory (DFT) further explored stability CA FA. It revealed strong interaction between carboxyl group hydrogen peroxide, suggesting reduced susceptibility oxidation. These findings provide foundation designing stable formulations enhancing understanding mechanisms phenolic

Language: Английский

Polyphenols: Potential Applications in Cancer Therapy

Molecular Nutrition & Food Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Polyphenols (PFs) are compounds found in fruits and vegetables, known for their health-related benefits, mainly including antioxidant, antiinflammatory, anticancer properties. However, efficacy is limited by poor bioavailability due to issues like low solubility, rapid metabolism, extensive excretion. Thus, research has focused on improving delivery systems, such as, example, nanoparticles, hydrogels, cocrystals, or conjugation with carrier molecules, which may protect PFs from degradation, improve and/or facilitate targeted cancer cells. promising modulating cancer-related pathways cell proliferation death, metastatic invasion, though translation patients hindered complex mechanisms. This review analyzes factors that affect PF bioavailability, evidences of vivo effects animal models mechanisms, results clinical trials, strategies enhance bioavailability. The idea need directly interact the challenged. Future aims optimize combine standard treatments, explore epigenetic effects, modulation tumor microenvironment, interactions gut microbiota. Advances personalized medicine structural modifications stability absorption could further potential. Despite challenges, remain a avenue complementary oncotherapy solutions.

Language: Английский

Relationships among osteoporosis, redox homeostasis, and alcohol addiction: Importance of the brain-bone axis

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 187, P. 118063 - 118063

Published: April 19, 2025

Language: Английский