In vitro nitric oxide inhibition of selected south African medicinal plants: A bio-guided purification of anti-inflammatory compounds from Conyza scabrida DOI Creative Commons

Sibusiso Rali,

Bongiwe Mshengu, Maryna van de Venter

et al.

Fitoterapia, Journal Year: 2025, Volume and Issue: 184, P. 106651 - 106651

Published: May 30, 2025

This study aimed to investigate the anti-inflammatory activity of plant species by in vitro screening extracts, fractions, and pure compounds on LPS-activated RAW 246.7 mouse macrophages. Plants were sourced from repository Biodiscovery Centre, at University Pretoria. The materials ground into fine powdered extracted with dichloromethane: methanol (DCM:MeOH) (1:1) followed 100 % MeOH, filtered, concentrated, dried generate extracts. Twelve extracts fractionated using positive pressure solid phase extraction (ppSPE) Gilson liquid hander workstation, resulting seven fractions per extract. observed was mainly attributed specific ppSPE rather than entire crude extract.The most active plants identified 90 inhibition 25 μg/mL, Dodonaea. viscosa, Buxus natalensis, Flacourtia. indica, Conyza scabrida. extract (6 7) C. scabrida demonstrated strong 50 μg/mL 97.8 97.0 inhibition, respectively. Ultra-Performance Liquid Chromatography coupled Quadrupole Time-of-Flight Mass Spectrometry (UPLC-QTOF-MS) analysis used tentatively identify two major 5,3',4'-trihydroxy-3,6,7,8-tetramethoxyflavone (C1) hautriwaic acid (C2) subsequently purified preparative high-performance chromatography (prep-HPLC-MS) confirmed Nuclear Magnetic Resonance (NMR). At a concentration μM, they inhibited NO production 96.6 59.2 %, We have provided scientific preliminary evidence supporting ethnopharmacological claims twelve South African medicinal traditionally treat inflammatory diseases.

Language: Английский

In vitro nitric oxide inhibition of selected south African medicinal plants: A bio-guided purification of anti-inflammatory compounds from Conyza scabrida DOI Creative Commons

Sibusiso Rali,

Bongiwe Mshengu, Maryna van de Venter

et al.

Fitoterapia, Journal Year: 2025, Volume and Issue: 184, P. 106651 - 106651

Published: May 30, 2025

This study aimed to investigate the anti-inflammatory activity of plant species by in vitro screening extracts, fractions, and pure compounds on LPS-activated RAW 246.7 mouse macrophages. Plants were sourced from repository Biodiscovery Centre, at University Pretoria. The materials ground into fine powdered extracted with dichloromethane: methanol (DCM:MeOH) (1:1) followed 100 % MeOH, filtered, concentrated, dried generate extracts. Twelve extracts fractionated using positive pressure solid phase extraction (ppSPE) Gilson liquid hander workstation, resulting seven fractions per extract. observed was mainly attributed specific ppSPE rather than entire crude extract.The most active plants identified 90 inhibition 25 μg/mL, Dodonaea. viscosa, Buxus natalensis, Flacourtia. indica, Conyza scabrida. extract (6 7) C. scabrida demonstrated strong 50 μg/mL 97.8 97.0 inhibition, respectively. Ultra-Performance Liquid Chromatography coupled Quadrupole Time-of-Flight Mass Spectrometry (UPLC-QTOF-MS) analysis used tentatively identify two major 5,3',4'-trihydroxy-3,6,7,8-tetramethoxyflavone (C1) hautriwaic acid (C2) subsequently purified preparative high-performance chromatography (prep-HPLC-MS) confirmed Nuclear Magnetic Resonance (NMR). At a concentration μM, they inhibited NO production 96.6 59.2 %, We have provided scientific preliminary evidence supporting ethnopharmacological claims twelve South African medicinal traditionally treat inflammatory diseases.

Language: Английский

Citations

0