Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide Survey

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 633 - 633

Published: March 11, 2025

Ash shoestring-associated virus (ASaV; Emaravirus fraxini) is a five-segmented, negative-sense RNA associated with chlorosis and leaf deformation in Fraxinus species. This study investigated the genetic diversity evolutionary dynamics of ASaV by analyzing nearly full-length RNA2–RNA5 partial RNA1 sequences from isolates collected different geographic regions hosts. The sequence data uncovered that has conserved genome, which RNA3 RNA5 showed more divergence than other segments sequenced isolates. were most informative genomic for phylogenetic studies. There was correlation between clustering host species when tree constructed based on region. genome predominantly under purifying selection. Newly designed primers this facilitated robust amplification regions.

Language: Английский

Designing the First Rosarium in Serbia to Fulfill Environmental, Societal, and Economical Purposes

Journal of Zoological and Botanical Gardens, Journal Year: 2024, Volume and Issue: 5(4), P. 590 - 605

Published: Oct. 8, 2024

Specialized botanical gardens such as a rose garden, or rosarium, play significant role due to their multifunctional nature surpassing simple gene bank assembly. Thus, this study conducted detailed analysis of garden through field and desk research, SWOT analysis, ecosystem services assessment, aiming determine the major strengths opportunities, well weaknesses threats that can promote constrain establishment first national rosarium in Serbia. After need for complete green area reconstruction arose achieve both attractiveness usefulness. Featuring old varieties, wild species, companies’ own specific collections, proposed has potential contribute reflected environmental, societal, economic purposes. Owing uniqueness breeding program collections created past decade, thematic parts—open greenhouse classroom, ‘roses under glass bell’, abundant river flow, pollinators’ shelter, taste scent garden—are envisaged future exemplary would not be only classroom but showroom interested nurseries, small-scale functional food producers, flower shops, amateur gardeners become aware new cultivars expand marketing utilization.

Language: Английский

Molecular Population Genetics of Aspen Mosaic-Associated Virus in Finland and Sweden

Viruses, Journal Year: 2023, Volume and Issue: 15(8), P. 1678 - 1678

Published: Aug. 1, 2023

Aspen mosaic-associated virus (AsMaV) is a newly identified Emaravirus, in the family Fimoviridae, Bunyavirales, associated with mosaic symptoms aspen trees (Populus tremula). are widely distributed Europe and understanding population structure of AsMaV may aid development better management strategies. The genome consists five negative-sense single-stranded RNA (-ssRNA) molecules. To investigate genetic diversity parameters AsMaV, different regions were amplified analyzed full-length sequence divergent isolates cloned sequenced. results show that RNA3 or nucleoprotein good representative for studying AsMaV. Developed RT-PCR-RFLP was able to identify areas higher number haplotypes could be applied screening large samples. In general, has conserved based on phylogenetic studies, geographical structuring observed from Sweden Finland, which attributed founder effects. under purifying selection but not uniformly genomic RNAs. Distant displayed amino acid variations compared other isolates, bioinformatic analysis predicted potential post-translational modification sites some viral proteins.

Language: Английский