Antibacterial Effect of Canine Leucocyte Platelet-Rich Plasma (L-PRP) and Canine Platelet-Poor Plasma (PPP) Against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus pseudintermedius DOI Creative Commons
Roberta Perego, Gabriele Meroni, Piera Anna Martino

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(12), P. 670 - 670

Published: Dec. 20, 2024

Staphylococcus pseudintermedius (SP) is a commensal and opportunistic pathogen of skin mucosal surfaces, isolated from healthy dogs canine pyoderma cases. It has recently gained attention due to its increasing antibiotic resistance. Platelet-rich plasma (PRP) biological product, obtained through blood centrifugation process, which antibacterial properties evidenced by in vitro vivo studies conducted both the human veterinary field. This study evaluated antimicrobial effect non-activated activated leucocyte-rich PRP (L-PRP) platelet-poor (PPP) against two strains SP with pyoderma: one multidrug-resistant strain (MDR) non-MDR strain. Twenty un-sedated adult donor were enrolled for L-PRP PPP production via closed semi-automatic system use. The evaluation was performed using micro-inhibition broth method, exposing 10 L-PRP, samples, respectively. Bacterial growth CFU count at three timepoints (immediately after incubation T0, 1 h T1 2 T2). had significant all similar MDR strains. Activation appeared reduce duration L-PRP. More are necessary confirm these preliminary results.

Language: Английский

Presence of Methicillin-Resistant Staphylococci and Carbapenemase-Positive Acinetobacter Isolates on Surfaces in German Dog Daycare Facilities and Correlation with Cleaning Practices DOI Creative Commons
Stephanie Forbes, Ellen Prenger‐Berninghoff, Christa Ewers

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(11), P. 568 - 568

Published: Nov. 15, 2024

Many bacteria, including methicillin-resistant staphylococci and opportunistic pathogens such as

Language: Английский

Citations

1
Future Challenge: Assessing the Antibiotic Susceptibility Patterns of Staphylococcus Species Isolated from Canine Otitis Externa Cases in Western Romania DOI Creative Commons
J. Dégi, Sorin Morariu,

Florin Simiz

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1162 - 1162

Published: Dec. 2, 2024

Introduction: Antimicrobial resistance (AMR) has surfaced as a critical challenge to public health on global scale. The precise and swift identification of antimicrobial agents, along with timely suitable therapy paired effective stewardship practices, is crucial for managing the rise dissemination resistance. objective our investigation was outline profile Staphylococcus spp., significant contributor canine otitis, prevalent condition in dogs, isolated Western Romania. Methods Materials: All data were collected from clinical cases otitis externa which presented at University Clinic Faculty Veterinary Medicine Timișoara/Romania. A evaluation conducted, era swabs are usually sent analysis laboratory. Laboratory included microbiological examination identifying spp. determining antibiotic susceptibility phenotypes. Statistical implemented all that collected. ear processed standard procedures cultivating bacteria. resulting subcultures determine staphylococcal species GP ID Cards Vitek® 2 automatic system. profiles detected by system using an AST-GP80 card. These strains further real-time PCR PCR-RFLP. Results: Of auricular exudate samples analyzed, 76 positive (59.38%). Within these, 82% samples, six distinct identified (Staphylococcus (S.) pseudintermedius, S. intermedius, hyicus, delphiny, shleiferi, aureus). Our indicate PCR-RFLP assay practical approach pseudintermedius identification, allowing discrimination other Intermedius Group (SIG) important pathogens dogs. highest frequency resistant isolates against tetracycline (21/34; 61.76%; p-value 0.003), gentamicin (20/34; 58.82%), kanamycin 58.82%). Conclusions: results essential guide prudent use antibiotics veterinary medicine. They will also help design efficient control strategies measure their effectiveness.

Language: Английский

Citations

1
Occurrence of Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus pseudintermedius colonization among veterinarians in the province of Malaga, Spain DOI Creative Commons

Fernando Fariñas-Guerrero,

Antonio J. Villatoro, Eduardo Martínez‐Manzanares

et al.

Veterinary World, Journal Year: 2024, Volume and Issue: unknown, P. 2719 - 2724

Published: Dec. 1, 2024

Staphylococcus pseudintermedius and aureus are common colonizing pathogens in companion animals. These opportunistic can cause infections of varying frequency severity humans pets. Studies on colonization veterinarians scarce. This study aimed to investigate the nostrils hands by S. aureus, epidermidis, among healthy clinical practice province Malaga (Spain), with a particular focus their potential antibiotic resistance. A request for voluntary participation was extended professionals from Official College Veterinarians Malaga. Nasal hand swabs were collected two trained technicians January 2024, all samples delivered laboratory within 24 h. Gram staining, catalase, oxidase, coagulase tests performed. The susceptibility isolated bacteria 11 antibiotics evaluated. total 50 enrolled study, comprising 36 women 14 men 31 veterinary clinics across Málaga province. 32% nasal yielded whereas 64% found contain epidermidis. In total, 30% participants did not exhibit any strains or hands. Two (11.1%) methicillin-resistant 18 nostrils. Furthermore, high prevalence resistant ampicillin (94.4%) amoxicillin (72.2%) observed. profiles similar those observed general population. Further research is required professionals, animals, owners better understand processes pet-human interface "One Health" approach.

Language: Английский

Citations

0
RECENT STUDY ON THE OTIC MICROBIOTA AND SUSCEPTIBILITY PROFILE TO ANTIMICROBIALS USED IN THE TREATMENT OF OTITIS IN PET CARNIVORES DOI
C. Rîmbu, C. Horhogea,

Daniel BEJINARIU

et al.

Scientific Papers Journal VETERINARY SERIES, Journal Year: 2024, Volume and Issue: 67(3), P. 130 - 138

Published: Dec. 13, 2024

In veterinary practice, otitis is the most commonly encountered condition in pet carnivores. Contributing factors and polyfactorial etiopathogenesis frequently lead to therapeutic failure medical interventions. Using a limited range of topical medicinal products intended for these conditions inevitably results resistance active substances. Our study, conducted on otic exudates (n=100) collected from dogs cats, highlighted complex polymicrobial etiology decrease sensitivity antimicrobials, culminating establishment multidrug phenomenon. vitro testing isolated bacterial strains across broad spectrum antimicrobials provided valid alternatives that prevented relapse. The obtained are useful practicing veterinarians highlight need continued research develop new options favorable prognosis.

Language: Английский

Citations

0
Antibacterial Effect of Canine Leucocyte Platelet-Rich Plasma (L-PRP) and Canine Platelet-Poor Plasma (PPP) Against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus pseudintermedius DOI Creative Commons
Roberta Perego, Gabriele Meroni, Piera Anna Martino

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(12), P. 670 - 670

Published: Dec. 20, 2024

Staphylococcus pseudintermedius (SP) is a commensal and opportunistic pathogen of skin mucosal surfaces, isolated from healthy dogs canine pyoderma cases. It has recently gained attention due to its increasing antibiotic resistance. Platelet-rich plasma (PRP) biological product, obtained through blood centrifugation process, which antibacterial properties evidenced by in vitro vivo studies conducted both the human veterinary field. This study evaluated antimicrobial effect non-activated activated leucocyte-rich PRP (L-PRP) platelet-poor (PPP) against two strains SP with pyoderma: one multidrug-resistant strain (MDR) non-MDR strain. Twenty un-sedated adult donor were enrolled for L-PRP PPP production via closed semi-automatic system use. The evaluation was performed using micro-inhibition broth method, exposing 10 L-PRP, samples, respectively. Bacterial growth CFU count at three timepoints (immediately after incubation T0, 1 h T1 2 T2). had significant all similar MDR strains. Activation appeared reduce duration L-PRP. More are necessary confirm these preliminary results.

Language: Английский

Citations

0