Science in One Health, Journal Year: 2025, Volume and Issue: unknown, P. 100111 - 100111
Published: April 1, 2025
Language: Английский
Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(3), P. 339 - 339
Published: March 6, 2024
As antimicrobial resistance (AMR) escalates globally, examining antibiotic treatment durations for respiratory infections becomes increasingly pertinent, especially in the context of COVID-19 pandemic. In a UK secondary care setting, this retrospective study was carried out to assess appropriateness durations-shorter (≤5 days) versus longer (6-7 days and >8 days)-for tract (RTIs) 640 adults across 2019 2020, accordance with local guidelines. The analysis employed these guidelines clinical evidence examine effectiveness suitability prescribing practices. This considered 'Shorter Is Better' approach, noting an increased rate patient discharges associated shorter regimens days). It further demonstrates that treatments are as effective ones conditions such COPD exacerbation, pneumonia, hospital-acquired pneumonia (HAP), except cases community-acquired (CAP) unspecified diagnoses. Nevertheless, raises concerns over observed increase mortality risk durations. Although differences were not statistically significant might have been influenced by pandemic, need extended research larger sample size is highlighted confirm findings. also emphasises critical accurate specific diagnoses considering assessments at admission, advocating tailored, evidence-based ensure safety. contributes stewardship efforts reinforcing importance adapting use current healthcare challenges promoting global commitment fight resistance. approach crucial enhancing outcomes saving lives on scale.
Language: Английский
Citations
5
Molecules, Journal Year: 2024, Volume and Issue: 29(13), P. 3120 - 3120
Published: June 30, 2024
The ongoing development of bacterial resistance to antibiotics is a global challenge. Research in that field thus necessary. Analytical techniques are required for such purpose. From this perspective, the focus was on atomic absorption spectrometry (AAS). Although it old, AAS often offers unexpected potential. Of course, should be exploited. aim therefore demonstrate versatility technique antibacterial research. This illustrated by various examples its practical application. can used, example, confirm identity compounds, purity controls, or quantify pharmaceutical preparations. latter allowed analysis without laborious sample preparation and interference from other excipients. In addition, help elucidate mode action mechanisms. context, quantifying accumulation antibiotic drug cell (resistant) bacteria appears play an important role. general application not limited metal-containing drugs, but also enables determination some organic chemical antibiotics. Altogether, perspective presents range applications research, intending raise awareness method may contribute fight against resistance.
Language: Английский
Citations
5
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: Dec. 14, 2023
Abstract This study reports the prevalence and antibiotics resistance status of Salmonella detected in raw meat from Lahore, Pakistan. Overall, N = 111 samples, were collected local markets. was recovered 57 (51.35%) including 45.83% poultry, 60% buffalo, 64.28% cow, goat samples. The predominant strains enterica serovars; Typhimurium (45.4%), Typhi (27.2%), Enteritidis (18.1%), identified by VITEK system 16S rRNA gene sequencing. isolates exhibited high to Erythromycin (100%), Cefepime (98.24%), Colistin (94.73%), Azithromycin (92.98%), Tetracycline (87.71%), Polymyxin B (84.21%), Ciprofloxacin Trimethoprim-Sulfamethoxazole (80.70%), Nalidixic Acid Kanamycin (78.94%), Chloramphenicol (77.19%), Streptomycin (71.92%) Ampicillin (64.91%). While more susceptibility Meropenem (75.43%) Amikacin (73.68%). 8 designated as Multidrug Resistant (MDR) 3 Extensively Drug-Resistant (XDR) . PCR-based detection genes revealed presence bla TEM-1 catA1 (64%), gyrA (18%). whole genome sequencing (WGS) two selected subsequent downstream analysis confirmed MDR XDR serovar Typhi. showed that consumed Lahore carries a significantly number drug-resistant Salmonella.
Language: Английский
Citations
12
Cellular and Molecular Biology, Journal Year: 2024, Volume and Issue: 70(2), P. 88 - 96
Published: Feb. 29, 2024
Language: Английский
Citations
4
Veterinary World, Journal Year: 2025, Volume and Issue: unknown, P. 202 - 209
Published: Jan. 1, 2025
Bovine mastitis, an inflammatory condition of the mammary gland, is a critical economic issue in dairy industry. Antimicrobial resistance (AMR) to mastitis-causing pathogens poses significant threat operations Thailand and Cambodia. This study investigates AMR mastitis It focuses on detecting methicillin-resistant Staphylococcus aureus, vancomycin-resistant S. aureus (VRSA), enterococci by identifying presence mecA, vanA, vanB genes bacterial isolates. A total 65 isolates (55 from 10 Enterococcus faecium Cambodia) were analyzed. Disk diffusion tests conducted for antibiotic susceptibility, polymerase chain reaction was employed detect genes. showed penicillin (21.8%), tetracycline (9.1%), gentamycin (7.3%). Three identified as multidrug-resistant (MDR), resistant tetracycline, gentamycin, penicillin. E. exhibited high (100%) (90%), with 60% classified MDR. Phenotypic analysis VRSA 11% However, not detected any isolate. Mastitis this pose challenges, especially MDR faecium, phenotypically without vanA gene. The findings highlight need judicious use farms further studies broader sampling.
Language: Английский
Citations
0
Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(2)
Published: Feb. 1, 2025
In the late 2000s, a pandemic of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) devastated kiwifruit orchards growing susceptible, yellow-fleshed cultivars. New Zealand's industry has since recovered, following deployment tolerant cultivar 'Zesy002'. However, little is known about extent to which Psa population evolving its arrival. Over 500 Psa3 isolates from Zealand were sequenced between 2010 and 2022, commercial monocultures diverse germplasm collections. While effector loss was previously observed on Psa-resistant vines, appears be rare in orchards, where dominant cultivars lack resistance. new variant, lost hopF1c, arisen. The hopF1c have been mediated by movement integrative conjugative elements introducing copper resistance into this population. Following variant's identification, in-planta pathogenicity competitive fitness assays performed better understand risk likelihood spread. variants had similar growth wild-type Psa3, lab-generated ∆hopF1c strain could outcompete wild type select hosts. Further surveillance conducted these originally isolated, with 6.6% surveyed identified as variants. These findings suggest that spread currently limited, they are unlikely cause more severe symptoms than current Ongoing genome biosurveillance recommended enable early detection management interest.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2329 - 2329
Published: March 5, 2025
DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin) is a C-type lectin receptor expressed on dendritic cells and M2 macrophages, playing key role in immune regulation pathogen recognition. Its ability to mediate anti-inflammatory effects by interacting with specific ligands triggers pathways that suppress pro-inflammatory responses promote tissue repair, making it potential therapeutic target for inflammatory autoimmune diseases. homologs various animal species share structural similarities perform comparable functions, offering valuable insights into its broader application across species. By recognizing carbohydrate pathogens, facilitates modulation, which can be harnessed developing therapies aimed at controlling inflammation. In veterinary medicine, diseases, such as rheumatoid arthritis bowel disease, represent significant challenges, the properties of could provide new options improve disease management enhance health. Future investigations should focus functional analysis species, well development preclinical models translate these findings clinical interventions bridging human
Language: Английский
Citations
0
Polimery, Journal Year: 2025, Volume and Issue: 70(3), P. 186 - 193
Published: March 27, 2025
To determine the optimal conditions for obtaining poly(lactic-co-glycolic) acid with selenium (PLGA/selenium) nanocomposites Taguchi method was used. FT-IR, Raman spectroscopy, XRD and FESEM analysis confirmed nanocomposites’ structure. The nanocomposite containing 6 mg/mL of obtained in process where mixing time 75 min showed highest antibacterial activity against Streptococcus mutans. are an innovative approach to improving oral health can be used as materials medical dental applications.
Language: Английский
Citations
0
Reports of Vinnytsia National Medical University, Journal Year: 2025, Volume and Issue: 29(1), P. 29 - 34
Published: March 20, 2025
Annotation. The global threat of bacterial resistance to antibiotics necessitates the search for alternatives and methods overcome resistance. aim this study was investigate antibacterial effect low-intensity electric current on multidrug-resistant P. aeruginosa strains determine its impact susceptibility antibiotics. studied reference clinical (n=15) in isotonic solution meat-peptone broth (MPB), with concentration measured using a densitometer. after exposure physical factor assessed serial dilution method minimum inhibitory (MIC) bactericidal (MBC). arithmetic mean (M), standard error (m), statistical significance (p) were calculated. As result solution, significant reduction observed compared untreated control: 1.3-fold decrease at 24 hours (p≤0.05) 2.38-fold 48 (p≤0.01). In MPB conditions, led cell by 1.36-fold (p ≤ 0.05) 1.42-fold hours. treatment reduced antibiotic 2- 4-fold. Without prior exposure, average MIC values ceftazidime 156.25±31.25 µg/mL, MBC - 479.17±122.55 µg/mL. For cefepime, 187.5±68.47 µg/mL 520.83±159.48 respectively. significantly decreased (p≤0.001) approximately 2.67-fold, 3.33-fold. cefepime 3.67-fold, 4.67-fold. resistant under influence currents expands understanding potential use weaken adaptive properties provides additional opportunities combating pathogen responsible infectious complications.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: April 12, 2025
Escherichia coli (E. coli) infections cause significant losses in the poultry industry and pose zoonotic risks due to rising antimicrobial resistance (AMR) virulence factors. This study investigates E. prevalence, AMR, genes (papC, vgrG1, iss) Egyptian chickens farm workers. A total of 35 dead from 14 flocks 17 workers urine samples were examined bacteriologically investigate presence followed by biochemical identification. Antimicrobial susceptibility testing was performed on antibiotics using disk diffusion method Mueller-Hinton agar, following 'Clinical Laboratory Standards Institute (CLSI) (2020) guidelines with Extended-spectrum β-lactamase (ESBL) activity evaluated via Double Disc Synergy Test (DDST) ceftazidime, cefotaxime, their clavulanate combinations CLSI protocols. detected through polymerase chain reaction (PCR) phylogenetic analysis vgrG1 gene genetic relatedness between chicken human isolates. The analysed 52 samples, identifying 18 organs (51.4%) 11 (64.7%), no difference. various antimicrobic sensitivity profiles identified phenotypically among all isolates which 29 isolates, 58.6% ESBL-producing, 96.5% exhibited multidrug (MDR), showing higher overall. similar proportions across highlighting public health resistant virulent coli. emphasized multidrug-resistant genes, potential transmission antibiotic use food safety.
Language: Английский
Citations
0