Development of Virus-Like Particles (VLPs) for Hepatitis C Virus genotype 4: a novel approach for vaccine development in Egypt
BMC Biotechnology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Jan. 18, 2025
Abstract
Background
Egypt
has
the
highest
global
prevalence
of
Hepatitis
C
Virus
(HCV)
infection,
particularly
genotype
4.
The
development
a
prophylactic
vaccine
remains
crucial
for
HCV
eradication,
yet
no
such
currently
exists
due
to
challenges.
ability
Virus-Like
Particles
(VLPs)
mimic
native
virus
and
incorporate
neutralizing
conformational
epitopes,
while
effectively
engaging
both
humoral
cellular
immune
responses,
makes
them
promising
approach
addressing
challenges
in
development.
Methods
Lentiviral-based
vectors
were
constructed
employed
integrate
full-length
sequence
Core,
E1,
E2,
P7
genes
4
into
genome
Human
Embryonic
Kidney
cells
(HEK293T).
Upon
expression,
structural
proteins
can
oligomerize
self-assemble
VLPs
mimicking
structure
virus.
purified
characterized
potential
VLPs-based
vaccine.
Results
In
this
study,
mammalian
successfully
engineered
stably
express
generate
non-infectious
expression
HCV-integrated
resulted
successful
production
proteins,
which
oligomerized
self-assembled
two
layers
enveloped
VLPs.
Electron
microscopy
analysis
revealed
spherical
particles
with
an
average
diameter
60–65
nm,
closely
resembling
mature
virions.
These
results
highlighted
these
as
candidate
Conclusions
underexplored
target
development,
despite
its
significant
public
health
burden,
especially
Egypt.
generation
represents
avenue
further
established
system
provides
robust
platform
study
VLP-based
vaccines
targeting
Language: Английский
A review of historical landmarks and pioneering technologies for the diagnosis of Hepatitis C Virus (HCV)
European Journal of Clinical Microbiology & Infectious Diseases,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 22, 2025
Language: Английский
Unlocking Potential: Virus-Like Particles as a Promising Strategy for Effective HCV Vaccine Development
Virology,
Journal Year:
2024,
Volume and Issue:
602, P. 110307 - 110307
Published: Nov. 15, 2024
Language: Английский
Advances in the Elimination of Viral Hepatitis in Mexico: A Local Perspective on the Global Initiative
Pathogens,
Journal Year:
2024,
Volume and Issue:
13(10), P. 859 - 859
Published: Oct. 1, 2024
Viral
hepatitis
(A–E)
presents
a
major
global
health
challenge.
In
2015,
the
World
Health
Organization
(WHO)
launched
an
initiative
to
eliminate
viral
hepatitis,
with
aim
of
reducing
new
infections
by
90%
and
deaths
65%
2030.
Mexico
is
one
38
focus
countries
identified
WHO,
collectively
accounting
for
80%
deaths.
While
B
C
are
commonly
diagnosed
in
Mexico,
routine
diagnosis
D
E
lacking,
no
specific
epidemiological
data
available.
2020,
implemented
National
Hepatitis
Elimination
Program,
focusing
on
preventing
infections,
complications
like
cirrhosis
hepatocellular
carcinoma,
ensuring
access
treatment,
improving
patient
care.
However,
this
program
has
not
been
extended
E.
Addressing
challenges
control
requires
increased
resource
allocation,
expanded
diagnosis,
vaccination
A
B,
treatment
coverage
C,
along
multisectoral
engagement.
This
work
provides
overview
Mexico’s
response
initiative,
highlighting
its
progress,
challenges,
areas
opportunity.
Language: Английский