Novel Insertion/Deletion Polymorphisms and Genetic Studies of the Shadow of Prion Protein (SPRN) in Raccoon Dogs DOI Creative Commons

Da-In Choi,

Mohammed Zayed, Eun‐Jee Na

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(24), P. 3716 - 3716

Published: Dec. 23, 2024

Prion diseases, or transmissible spongiform encephalopathies (TSEs), are a group of invariably fatal neurodegenerative disorders. One the candidate genes involved in prion diseases is shadow protein (SPRN) gene. Raccoon dogs, canid, considered to be disease-resistant species. To date, genetic polymorphisms SPRN gene and predicted structure (Sho) have not been explored raccoon dogs. was amplified using polymerase chain reaction (PCR). We also investigated by analyzing frequencies genotypes, alleles, haplotypes, as well linkage disequilibrium among identified variations. In addition, silico analysis with MutPred-Indel performed predict pathogenicity insertion/deletion polymorphisms. Predicted 3D structures were analyzed Alphafold2. found total two novel synonymous single nucleotide three Sho dogs resemble that This first study regarding structural characteristics dog

Language: Английский

Unseen Dangers—The Role of Invasive Species in the Spread of Zoonotic Diseases in Europe DOI Creative Commons
Myriam Kratou, Alejandro Cabezas‐Cruz

Pathogens, Journal Year: 2025, Volume and Issue: 14(2), P. 159 - 159

Published: Feb. 7, 2025

In a recent study, Klink et al [...]

Language: Английский

Citations

0
Tick-borne pathogens in raccoons (Procyon lotor) from Germany DOI Creative Commons

Lara M I Heyse,

Nina Król, Zaida Rentería‐Solís

et al.

Ticks and Tick-borne Diseases, Journal Year: 2025, Volume and Issue: 16(2), P. 102457 - 102457

Published: Feb. 13, 2025

Language: Английский

Citations

0
In situ collected small intestinal mucosal swabs as alternative analytes to diagnose Echinococcus multilocularis infection in its definitive hosts by real-time polymerase chain reaction DOI Creative Commons

Alrik-Markis Kunisch,

Josefine Wassermann,

Martin C. Peters

et al.

Veterinary Parasitology, Journal Year: 2025, Volume and Issue: 335, P. 110418 - 110418

Published: Feb. 14, 2025

The cestode Echinococcus multilocularis parasitizes small intestines of carnivores such as foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) definitive hosts represents an important zoonotic threat. This study aimed to determine whether E. DNA detection methods by real-time PCR involving easy-to-collect sample types, intestinal swabs could represent alternatives traditional parasitological techniques. Three analytes from were tested. i. Swabs taken immediately during necropsy (in situ) the mucosa (FastMucSwab), ii. scraped-off (IsolMucSwab), collected for sedimentation-counting-technique (SCT) comparative reasons iii. feces Ampulla recti. Only minor differences in detection, independent type, observed. High levels concordance between results noted comparison on FastMucSwabs, IsolMucSwabs or feces. agreement FastMucSwab IsolMucSwab was excellent (Kappa 0.86 [95 % CI: 0.79-0.92]). If inconclusive excluded, FastMucSwabs had a diagnostic sensitivity 92.7 (95 83.0-97.3 %) specificity 91.5 88.4-93.9 relative SCT. Compared with SCT, Feces 82.8 63.5-93.5 90.9 81.4-95.9 %). Segmental swabbing no advantages. Overall, there evidence that used here higher than SCT did. Results suggest simple alternative methods, efficient tool performing larger-scale epidemiological studies.

Language: Английский

Citations

0
Molecular Evidence of Raccoon Dog (Nyctereutes procyonoides) as a Natural Definitive Host for Several Sarcocystis Species DOI Creative Commons
Petras Prakas, Т.В. Калашникова, Naglis Gudiškis

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(3), P. 288 - 288

Published: March 15, 2025

Sarcocystis parasites infect a wide range of animals, including reptiles, birds, and mammals, have complex two-host prey–predator life cycle. Sarcocysts are mainly found in the muscles intermediate hosts, oocysts sporulate intestines definitive host. The raccoon dog (Nyctereutes procyonoides), native to Asia invasive Europe, is known disease carrier. However, studies on dogs transmission scarce. Between 2019 2024, total 26 carcasses were collected Lithuania. results light microscopy examination indicated that 50% samples positive for spp. sporocysts sporulated oocysts. Based nested PCR sequencing cox1, 88.5% these parasites. Molecular analysis revealed presence 11 different species. Eight species, S. alces, capracanis, hjorti, iberica, linearis, morae, tenella, venatoria reported first time as hosts. identified species linked such cervids, wild boars, pigs, goats, sheep. These findings suggest play key role spread Sarcocystis, particularly infecting cervids.

Language: Английский

Citations

0
Pathological findings in raccoon dogs (Nyctereutes procyonoides) in Schleswig-Holstein, Germany DOI Creative Commons
Jana C. Klink, Alexandra Rieger, Peter Wohlsein

et al.

Journal of Comparative Pathology, Journal Year: 2025, Volume and Issue: 219, P. 59 - 77

Published: May 1, 2025

Language: Английский

Citations

0
Haematochemical Profile of Healthy Dogs Seropositive for Single or Multiple Vector-Borne Pathogens DOI Creative Commons
Raffaella Cocco, Sara Sechi, Maria Antonietta Rizzo

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(5), P. 205 - 205

Published: May 8, 2024

the present study aimed to investigate immunological response common vector-borne pathogens and evaluate their impact on haematochemical parameters in owned dogs.

Language: Английский

Citations

1
Successful Treatment of Scabies-Induced Life Threatening Anemia in a Wild Raccoon Dog (Nyctereutes procyonoides) DOI Creative Commons
Myeongsu Kim, Phyo Wai Win, Yoonhee Kim

et al.

Journal of Veterinary Clinics, Journal Year: 2024, Volume and Issue: 41(3), P. 195 - 199

Published: June 30, 2024

A free-range wild raccoon dog (Nyctereutes procyonoides) was rescued with cachexia.Physical examination revealed generalized hyperkeratosis and alopecia typical of scabies as well hypothermia (35.6°C).The patient obtunded severely dehydrated (10%).Hematological parameters included a low packed cell volume (PCV; 15%) hemoglobin concentration, leukocytosis, hypoglycemia.A blood smear different subtypes hypochromic leptocytes, indicating regenerative response against severe anemia.This case initially tentatively diagnosed anemia due to chronic external bleeding presumed be caused by scabies-induced skin injuries.Darbepoetin alpha (DPO), iron dextran, fluralaner were administered at the initial presentation, supportive care including oxygen supplementation, warming, nutritional support provided.However, on day 5, PCV dropped 5.9% presumably rapid rehydration drinking water ad libitum.DPO boosted days 5 6 along daily dextran.On 21, had recovered 19.8%, evaluation showed strong response.This shows that even if occurs in dog, it can managed an appropriate response.In particular, since rate food intake is faster than hematopoietic dogs, may decrease rapidly early stage treatment; therefore, diagnostic additional medical management for hematopoiesis are necessary.

Language: Английский

Citations

0
Novel Insertion/Deletion Polymorphisms and Genetic Studies of the Shadow of Prion Protein (SPRN) in Raccoon Dogs DOI Creative Commons

Da-In Choi,

Mohammed Zayed, Eun‐Jee Na

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(24), P. 3716 - 3716

Published: Dec. 23, 2024

Prion diseases, or transmissible spongiform encephalopathies (TSEs), are a group of invariably fatal neurodegenerative disorders. One the candidate genes involved in prion diseases is shadow protein (SPRN) gene. Raccoon dogs, canid, considered to be disease-resistant species. To date, genetic polymorphisms SPRN gene and predicted structure (Sho) have not been explored raccoon dogs. was amplified using polymerase chain reaction (PCR). We also investigated by analyzing frequencies genotypes, alleles, haplotypes, as well linkage disequilibrium among identified variations. In addition, silico analysis with MutPred-Indel performed predict pathogenicity insertion/deletion polymorphisms. Predicted 3D structures were analyzed Alphafold2. found total two novel synonymous single nucleotide three Sho dogs resemble that This first study regarding structural characteristics dog

Language: Английский

Citations

0