Cultural human-snake interactions in the festa dei serpari as an opportunity for the surveillance of bacterial pathogens of zoonotic concern DOI Creative Commons
Renata Fagundes‐Moreira,

Domenico Otranto,

Gianpaolo Montinaro

et al.

Comparative Immunology Microbiology and Infectious Diseases, Journal Year: 2025, Volume and Issue: 118, P. 102325 - 102325

Published: Feb. 12, 2025

Reptiles harbour a wide range of pathogen species, some which can pose risks to human health. Among them, snakes serve as valuable sentinels for monitoring pathogens, particularly in cultural contexts where animal-human interactions offer unique opportunity delve into bacterial dynamics throughout the years. This is case annual serpent ritual "festa dei serpari" Cocullo, central Italy renowned San Domenico festival. study conducted retrospective analysis comprehensive database pathogens associated with wild captured during ritual, from 2010 2023 (excluding 2020 and 2021). Using different sampling methods, comprising cloacal, oral, cutaneous swabs, along faecal material, total 563 biological samples were collected 465 registered harvested this ritual. Samples analysed assess prevalence species on potential Seventy identified using traditional growth media, molecular diagnosis, biochemical analyses, mass spectrometry performed two national reference institutes. results, zoonotic opportunistic bacteria such Salmonella spp., Escherichia coli, drug-resistant Pseudomonas aeruginosa Klebsiella pneumoniae particular concern. The results also revealed significant trends years under investigation, an increasing Gram-negative Staphylococcus sciuri, decline spp. Our findings emphasize importance continued surveillance given close human-snake that occur Sacro-profane historical

Language: Английский

Molecular detection and phylogeny of Hepatozoon ophisauri and Toxoplasma gondii in wild lizards from Khyber Pakhtunkhwa, Pakistan DOI

Sidra Ghafar,

Asmat Ullah Khan,

Mustansar Khalid

et al.

Folia Microbiologica, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

Language: Английский

Citations

1
Recombinant production in Escherichia coli of functionally active alpha-hemolysin from the human pathogen Staphylococcus aureus DOI Creative Commons

Jaime L. Díaz-Varela,

Vittoria Sabia,

Diego Heras‐Márquez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract Staphylococcus aureus is a human opportunistic pathogen capable of causing multiple infections in both humans and animals. It secretes group exotoxins, known as hemolysins, which are released to enhance its pathogenicity. All them exhibit cytolytic activity on variety host cell types, but α-hemolysin stands out for being the most thoroughly studied variant. In this work, we show production purification S. following straightforward protocol sufficient quantity consider it potential procedure future biotechnological approaches. Functional structural characterization has indeed revealed that protein fully functional, confirming key role cholesterol necessary protein-lipid interaction. Furthermore, also been shown purified toxin can be assembled into single-particle individual pores within soluble lipid platforms form cholesterol-containing nanodiscs.

Language: Английский

Citations

0
Cultural human-snake interactions in the festa dei serpari as an opportunity for the surveillance of bacterial pathogens of zoonotic concern DOI Creative Commons
Renata Fagundes‐Moreira,

Domenico Otranto,

Gianpaolo Montinaro

et al.

Comparative Immunology Microbiology and Infectious Diseases, Journal Year: 2025, Volume and Issue: 118, P. 102325 - 102325

Published: Feb. 12, 2025

Reptiles harbour a wide range of pathogen species, some which can pose risks to human health. Among them, snakes serve as valuable sentinels for monitoring pathogens, particularly in cultural contexts where animal-human interactions offer unique opportunity delve into bacterial dynamics throughout the years. This is case annual serpent ritual "festa dei serpari" Cocullo, central Italy renowned San Domenico festival. study conducted retrospective analysis comprehensive database pathogens associated with wild captured during ritual, from 2010 2023 (excluding 2020 and 2021). Using different sampling methods, comprising cloacal, oral, cutaneous swabs, along faecal material, total 563 biological samples were collected 465 registered harvested this ritual. Samples analysed assess prevalence species on potential Seventy identified using traditional growth media, molecular diagnosis, biochemical analyses, mass spectrometry performed two national reference institutes. results, zoonotic opportunistic bacteria such Salmonella spp., Escherichia coli, drug-resistant Pseudomonas aeruginosa Klebsiella pneumoniae particular concern. The results also revealed significant trends years under investigation, an increasing Gram-negative Staphylococcus sciuri, decline spp. Our findings emphasize importance continued surveillance given close human-snake that occur Sacro-profane historical

Language: Английский

Citations

0