Urobiota and urinary tract infections in liver cirrhosis

Сибирский научный медицинский журнал, Journal Year: 2025, Volume and Issue: 45(1), P. 148 - 157

Published: Feb. 27, 2025

The urobiome is being actively studied, diagnostics are improved and new markers of inflammatory diseases the urinary tract searched for, despite fact that most microorganisms unidentified their functions remain not fully understood. aim study was to composition microbiota (urobiota) in patients with liver cirrhosis, depending on presence infection. Material methods. A prospective cohort single-center conducted 48 cirrhosis (30 men, 18 women), who, addition standard studies, underwent metagenomic sequencing urine feces. average age examined 50.5 years, there were 16 32 without infection, respectively. High-performance performed using MiSeq genetic analyzer (Illumina, USA) a protocol based analysis variable regions 16s rRNA gene. Data Kraken2 algorithm. significance level α assumed be 0.05. registered Clinicaltrials.gov (NCT05335213). results discussion. dominant phylotypes urobiota Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, among which Proteobacteria predominate (more than 50 %). beta diversity has significant differences or infection (p = 0.001). Urinary increases density such taxa as Gammaproteobacteria, including Escherichia, Klebsiella, Acinetobacter, Enterobacter, well Bacilli, Synergistia, Deltaproteobacteria, Epsilonproteobacteria, Acidithiobacillia decreases Prevotella, Clostridioides, Brevundimonas, Delftia, Stenotrophomonas, Streptococcus < 0.05). Conclusions. Microbiological identification method made it possible identify more 1,000 types uncultivated ones, characteristic bacterial pattern expands understanding pathogenesis diagnostic possibilities infections creates prerequisites for substantiating directions modulation personalization patient treatment.

Language: Английский

The influence and relationship of dysbiosis in the urinary microbiota on patients with urolithiasis

Urolithiasis, Journal Year: 2025, Volume and Issue: 53(1)

Published: March 19, 2025

Language: Английский

Gut Microbiome Implication and Modulation in the Management of Recurrent Urinary Tract Infection

Pathogens, Journal Year: 2024, Volume and Issue: 13(12), P. 1028 - 1028

Published: Nov. 21, 2024

Urinary tract infections (UTIs) are one of the most common bacterial infections, affecting more than 150 million people each year in world. UTIs have grown exponentially last few years. They represent a major load for both individuals and society. The highest incidence (about 55–60%) concerns women. Many pathogens involved UTIs, which derived from gut. Recent studies, together with recent diagnostic techniques (such as quantitative culture urine or next-generation sequencing), improved knowledge microbial communities urinary tract. It turned out that gut dysbiosis is strictly pathogenesis UTIs. In particular, human natural habitat Escherichia coli (E. coli), main bacterium responsible overgrowth E. pathogenic strains represents risk factor them. Furthermore, microbiota acts “global reservoir” genes conferring resistance to clinically relevant antibiotics, thus influencing treatment addition, differently past, idea sterile environment has been replaced by characterization microbiome. aim our review explore studies on association between microbiome summarize current about effects interactions considering “gut disease” not only disease providing new insight into therapeutic options such use probiotics.

Language: Английский