Green Fabrication of Zinc Oxide Nanoparticles Using Phlomis Leaf Extract: Characterization and In Vitro Evaluation of Cytotoxicity and Antibacterial Properties

Molecules, Journal Year: 2021, Volume and Issue: 26(20), P. 6140 - 6140

Published: Oct. 11, 2021

Green nanoparticle synthesis is an environmentally friendly approach that uses natural solvents. It preferred over chemical and physical techniques due to the time energy savings. This study aimed synthesize zinc oxide nanoparticles (ZnO NPs) through a green method used Phlomis leaf extract as effective reducing agent. The characterization of ZnO NPs were confirmed by UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Dynamic light scattering (DLS), Zeta potential, Field Emission Scanning Electron Microscope (FESEM) techniques. In vitro cytotoxicity was determined in L929 normal fibroblast cells using MTT assay. antibacterial activity investigated disk-diffusion against S. aureus E. coli, well minimum inhibitory concentration (MIC) bactericidal (MBC) content concentrations. XRD results nanoparticles' crystalline structure. Nanoparticle sizes found be around 79 nm FESEM, whereas hydrodynamic radius estimated 165 ± 3 DLS. FTIR spectra revealed formation bonding surfactant molecule adsorption on surface NPs. interesting observe aqueous extracts leave plant are efficient agents for vitro, with no cytotoxic effect significant impact bacteria tested.

Language: Английский

---

Synthesis and characterization of silver nanoparticles loaded with carboplatin as a potential antimicrobial and cancer therapy

Cancer Nanotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 6, 2024

Abstract In recent studies with silver nanoparticles, it has been reported that the use of nanoparticles in carrier drug systems increases tumor suppression and reduces drug-related side effects. At same time, combination traditional medicine nanotechnology provides opportunity to develop new antimicrobial agents. The aim this study was determine anticancer, activities pro-apoptotic effects (AgNPs), carboplatin-loaded (AgNPs-Car). Characterization synthesized were carried out by DLS, EDX-STEM, FTIR analysis. antiproliferative these molecules evaluated using XTT Annexin V, respectively. MIC (Minimum Inhibitory Concentration) test used activity. anticancer activity AgNPs-Car high MCF-7 (human breast adenocarcinoma), A549 lung carcinoma), C6 (brain glioma) cells. cell group most effective selective cytotoxic It also shown AgNPs induced DNA fragmentation eventually increasing apoptosis on Gram-positive Gram-negative pathogenic microorganisms yeast fungi. Among nanomaterials reached values according reference sources, achieved better results. As a result, found be very successful targeting glioma cells facilitating entry drug. addition, their toxicity low. Silver are preferred for creating system because qualitative properties Therefore, is an interesting field research cancer microorganisms.

Language: Английский

---

Fabrication, characterization and application of biocompatible nanocomposites: A review

European Polymer Journal, Journal Year: 2024, Volume and Issue: 214, P. 113187 - 113187

Published: June 1, 2024

Language: Английский

---

Laser ablated Au@Ag@Au core double shelled as inorganic nanocomposite for control growth of liver cancer cells (Hep-G2): 2D tissue culture model and insilico assessment

Inorganic Chemistry Communications, Journal Year: 2025, Volume and Issue: unknown, P. 113841 - 113841

Published: Jan. 1, 2025

Language: Английский

---

Layer-by-Layer Nanoparticles of Tamoxifen and Resveratrol for Dual Drug Delivery System and Potential Triple-Negative Breast Cancer Treatment

Pharmaceutics, Journal Year: 2021, Volume and Issue: 13(7), P. 1098 - 1098

Published: July 20, 2021

Nanoparticle development demonstrates use in various physicochemical, biological, and functional properties for biomedical applications, including anti-cancer applications. In the current study, a cancer therapeutic conjugate was produced consisting of tamoxifen (TAM) resveratrol (RES) by layer-by-layer (LbL) nanoparticles based on lipid-based drug delivery systems liquid crystalline (LCNPs) coated with multiple layers positively charged chitosan negatively hyaluronic acid evaluation biocompatibility against cells. Multiple techniques characterized synthesis TAM/RES–LbL-LCNPs, such as Fourier-transform infrared spectroscopy (FTIR), X-ray crystallography (XRD), Zeta potential analysis, particle size Field Emission Scanning Electron Microscope (FESEM), Transmission electron microscopy (TEM). The vitro cytotoxic effects TAM/RES–LbL-LCNPs were investigated human breast cell line, Michigan Cancer Foundation-7 (MCF-7), triple-negative Centre Antoine Lacassagne-51 (CAL-51), using parameters. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed that treatment cells caused reduction proliferation, no inhibition observed normal liver line: American Type Culture Collection Cell Line-48 (WRL-68 [ATCC CL-48]). Fluorescent examined ability Fluorescein isothiocyanate (FITC) to bind along their cellular uptake. Apoptosis determination performed hematoxylin–eosin acridine orange–propidium iodide double staining. expression P53 caspase-8 analyzed flow cytometry analysis. An vivo study determined toxicity mice assessed marker changes kidneys. No significant statistical differences found tested indicators. TAM/RES–LbL-LCNP showed apparent damages or histopathological abnormalities heart, lung, liver, spleen, kidney histological images. findings first time propose provide new safer method phytochemicals combinatorial therapy novel approach cancers.

Language: Английский

---

Biogenic copper oxide nanoparticles from Bacillus coagulans induced reactive oxygen species generation and apoptotic and anti-metastatic activities in breast cancer cells

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Feb. 24, 2023

The present study examined the anticancer capabilities of Bacillus coagulans supernatant-produced copper oxide nanoparticles (BC-CuONPs) on MCF-7 and SKBR3 cancer cells. X-ray diffraction, ultraviolet-visible spectroscopy, Fourier-transform infrared photoelectron transmission electron microscopy, field-emission scanning energy-dispersive X-ray, dynamic light scattering, zeta potential techniques were used to characterize BC-CuONPs. This also investigated cellular molecular processes NPs' anti-proliferative apoptotic properties human breast cells compared them commercial pharmaceutical tamoxifen. size spherical NP was from 5 47 nm with negative potential. MTT results showed great cytotoxic effect BC-CuONPs against inhibited growth in a time- dose-dependent manner. up-regulation BCL2-associated X (BAX), cyclin dependent kinase inhibitor 1A (P21), Caspase 3 (CASP3), 9 (CASP9), down-regulation BCL2 apoptosis regulator (BCL2), Annexin V-FITC/propidium iodide, reactive oxygen species (ROS) generation suggested that had significant impact when control. Scratch tests vascular endothelial factor receptor gene (VEGF) demonstrated anti-metastatic activity. cell cycle analysis Cyclin D1 (CCND1) 4 (CDK4) revealed arrested sub-G1 phase. Finally, secondary metabolites supernatant could form CuONPs, biogenic anti-metastasis while having less adverse effects normal Therefore, synthesized CuONPs using B. can be shown as candidate for new therapeutic strategy management.

Language: Английский

---

Performance SiO2, GO, and SiO2@GO nanomaterials on fabricating new polymer nanocomposites for optical, antibacterial, and anticancer applications

Applied Nanoscience, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 11, 2025

Language: Английский

---

Gold nanoparticles loaded TNF-α and CALNN peptide as a drug delivery system and promising therapeutic agent for breast cancer cells

Materials Technology, Journal Year: 2022, Volume and Issue: 37(14), P. 3152 - 3166

Published: Oct. 13, 2022

We investigated the anti-cancer properties of gold nanoparticles loaded TNF- and CALNN peptides, which we proposed as a potential drug delivery system using in vitro vivo models. The binding GNPs-TNF- GNPs-TNF-CALNN was characterized UV, ELISA SEM analysis. outcomes demonstrated that novel had an anti-proliferative activity against breast cancer cell lines through mechanism apoptosis induction. In model involved studying cytotoxic influence GNPs, GNPs-TNF-α GNPs-TNF-α-CALNN when applied to transplanted AN-3 line. tumor sections were examined microarray. In-vivo studies GNPs alone less growth inhibitory effect on tumors implanted mice compared GNPs-TNF – combined therapy. assay showed exhibit selective toxicity towards cells, inducing activation caspase-3 7, p53 protein.

Language: Английский

---

Laser-Ablated Zinc Oxide Nanoparticles and Evaluation of Their Antibacterial and Anticancer Activity Against an Ovarian Cancer Cell Line: In Vitro Study

Plasmonics, Journal Year: 2023, Volume and Issue: 18(6), P. 2091 - 2101

Published: July 6, 2023

Language: Английский

---

Facile synthesis of Fe2O3, Fe2O3@CuO and WO3 nanoparticles: characterization, structure determination and evaluation of their biological activity

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 13, 2024

Abstract Due to their high specific surface area and its characteristic’s functionalized nanomaterials have great potential in medical applications specialty, as an anticancer. Herein, functional nanoparticles (NPs) based on iron oxide Fe 2 O 3 , modified with copper @CuO, tungsten WO were facile synthesized for biomedical applications. The obtained nanocrystal sizes of 35.5 nm 7 25.5 . In addition octahedral square nanoplates 3; respectively. Results revealed that NPs showed remarked anticancer effects versus a safe effect normal cells through cytotoxicity test using MTT-assay. Notably, e.g. our result demonstrated @CuO exhibited the lowest IC 50 value MCF-7 cancer cell line at about 8.876 µg/ml, compared was 12.87 µg/ml 9.211 which indicate modification gave highest antiproliferative against breast cancer. However, these mode toward Vero line, where monitored 40.24 21.13 25.41 NPs. For further evidence. antiviral activity virucidal viral adsorption mechanisms practiced by mechanism prevented virus from replicating inside cells. complete reduction load synergistic combinations between tested two materials instead alone. Interestingly, antimicrobial efficiency NPs, evaluated E. coli, S. aureus, C. albicans pathogens. widest microbial inhibition zone ( ca. 38.45 mm) observed 250 mg/ml coli whereas 40 NPS could form 32.86 mm aureus Nevertheless, relatively resistant all examined superior activities nanostructures might be due unique features accepted evaluations.

Language: Английский

---