Journal of Applied Physics, Journal Year: 2023, Volume and Issue: 133(12)
Published: March 24, 2023
First Page
Language: Английский
Molecules, Journal Year: 2024, Volume and Issue: 29(5), P. 1077 - 1077
Published: Feb. 29, 2024
Raman spectroscopy is an emerging method for the identification of bacteria. Nevertheless, a lot different parameters need to be considered establish reliable database capable identifying real-world samples such as medical or environmental probes. In this review, establishment databases with proper design in microbiological studies demonstrated, shining light into all parts that require attention. Aspects strain selection, sample preparation and isolation requirements, phenotypic influence, measurement strategies, well statistical approaches discrimination bacteria, are presented. Furthermore, influence these aspects on spectra quality, result accuracy, read-out discussed. The aim review serve guide can support fields.
Language: Английский
Citations
4
Analytical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15
Published: Feb. 26, 2024
Antibodies are the most common ligands in commercial and research assay systems for detecting whole pathogen cells. On other hand, aptamers superior with many advantages over antibodies sensitive robust development. Extensive comparisons between aptamer-based biosensors immunosensors limited to protein analytes. Here, we report a comparison of (four one aptamer each bacteria) be used as biosensor Escherichia coli Staphylococcus aureus on glass surfaces through systematic experiments. We have demonstrated that anti-E. antibody mouse monoclonal S. best performance among compared ligands. Hence, were further investigated within scope linear range, analytical sensitivity, reproducibility results. capture efficiency 89.1% 88.2% The results suggest function higher than but strong potential an tool.
Language: Английский
Citations
3
Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 116784 - 116784
Published: April 1, 2025
Language: Английский
Citations
0
Journal of Biomechanical Engineering, Journal Year: 2024, Volume and Issue: 146(9)
Published: March 21, 2024
Immunoassays based on reactions between target pathogen (antigen; Ag) and antibody (Ab) are frequently used for Ag detection. An external magnetic field was to immobilize microbeads-tagged-antibodies (mMB-Ab) the surface of a microchannel in capture zone. The mMB-Ab subsequently objective this numerical study, with experimental validation, is assess reaction presence electro-osmotic flow (EOF). First, immobilization complex wall zone achieved. Subsequently, transported by EOF toward bind immobilized mMB-Ab. Lastly, mMB-Ab:Ag formed A finite volume solver implement above steps. investigated electric fields (E): 150 V/cm-450 V/cm concentrations: 0.001 M-1000 M. depletion increases time as E decreases. Furthermore, concentration decreases, time. These results quantify detection using device; thus, signifying its potential rapid throughput screening Ag. This platform technology can lead development portable devices cells, pathogens, biomolecules testing water systems, biological fluids, biochemicals.
Language: Английский
Citations
2
Medicina, Journal Year: 2023, Volume and Issue: 59(10), P. 1719 - 1719
Published: Sept. 26, 2023
Background and Objectives: Staphylococcus aureus is a prevalent bacterium capable of inducing various infections, including skin soft tissue bloodstream pneumonia, surgical site infections. The emergence antimicrobial resistance in S. aureus, particularly methicillin-resistant has raised substantial concerns within global healthcare settings. Prior to antibiotic prescription, the ideal approach susceptibility testing (AST); however, this frequently perceived as excessively complex time-intensive. Lab-on-a-chip (LOC) technology holds promise addressing these challenges advancing fundamental microbiological research while also aiding development therapeutic strategies. This systematic review aims evaluate potential utility LOC for AST aureus. Materials Methods: study adhered PRISMA guidelines. Various databases, SCOPUS, PubMed/MEDLINE, SCIELO, LILACS, addition gray literature sources, were employed process. Results: Sixteen studies included review. All detailed effectiveness, rapidity, predictability systems assessing antibiotics. When comparing traditional manual methods, it was evident that requires minimal quantity reagents. Furthermore, most reported entire procedure took 10 min 7 h, with results being equally accurate those obtained through protocols. Conclusions: application emphasized by its ability provide rapid access minimum inhibitory concentration data, which can substantially aid selecting suitable antibiotics dosages treating challenging infections caused microorganism. Moreover, facilitated enhancing appropriateness efficacy therapy clinical
Language: Английский
Citations
5
Sensors, Journal Year: 2024, Volume and Issue: 24(14), P. 4503 - 4503
Published: July 11, 2024
Due to its high spatial resolution, Raman microspectroscopy allows for the analysis of single microbial cells. Since spectroscopy analyzes whole cell content, this method is phenotypic and can therefore be used evaluate cellular changes. In particular, labeling with stable isotopes (SIPs) enables versatile use observation different metabolic states in microbes. Nevertheless, static measurements only analyze present situation do not allow further downstream evaluations. Therefore, a combination sorting necessary provide possibility research on selected bacteria sample. Here, new microfluidic approach Raman-activated continuous-flow using an optical setup image-based particle synchronous acquisition spectra making decision demonstrated, showing that active cells successfully sorted by means chip.
Language: Английский
Citations
1
International Journal of Science and Research (IJSR), Journal Year: 2024, Volume and Issue: 13(2), P. 656 - 660
Published: Feb. 5, 2024
Recently, and with the help of some newfangled techniques such as DNA\RNA testing (NAT), many microorganisms have been diagnosed accurately quickly we their strains can be distinguished easily. Therefore, this study was prepared to design primers molecular laboratory tests for a specific gene that is found in bacteria Escherichia coli O157: H7, which has obtained from stored foods these were designed using primer software. In study, distinctive unique region within genetic material particular organism selected template (i. e. sxt 1 H7 selected), then suitable prepare RAPD -PCR enable amplification region, investigate it by migration gel electrophoresis technique. The purpose technique simplicity its work low costs, addition rather fast effectiveness discovering constantly form new coli. identification results showed (NAT) analysis put specific, sensitive rapid method revelation epidemiology food -borne infections.
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 143 - 172
Published: Jan. 1, 2024
Language: Английский
Citations
0
Journal of Applied Physics, Journal Year: 2023, Volume and Issue: 133(12)
Published: March 24, 2023
First Page
Language: Английский
Citations
0