Antimicrobial Peptides and Their Assemblies DOI Creative Commons
Ana Maria Carmona‐Ribeiro

Future Pharmacology, Journal Year: 2023, Volume and Issue: 3(4), P. 763 - 788

Published: Oct. 19, 2023

Antibiotic resistance requires alternatives to fight multi-drug resistant strains. Antimicrobial peptides (AMPs) act by disrupting or solubilizing microbial cell walls membranes in accordance with mechanisms difficult counteract from the microbe’s point of view. In this review, structure–activity relationships for AMPs and their assemblies are discussed, considering not only self-assembly but also interactions carriers optimal delivery combinations other complementary antimicrobials moieties covalently bound chemical structure. The effect formulations on AMP activity is evaluated, revealing a myriad possibilities. Depending interaction forces between AMP, carrier, elements added formulations, can be reduced, enhanced, remain unaffected. Approaches protecting against proteolysis may reduce activity.

Language: Английский

Colloidal Dispersions of Gramicidin D in Water: Preparation, Characterization, and Differential Cytotoxicity DOI Creative Commons
Ricardo Márcio-e-Silva,

Bianca Reche Bazan,

Rodrigo Tadeu Ribeiro

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

Gramicidin D (Gr) is a natural mixture of channel peptides A-C with minor differences in chemical structure, which are able to span cell membranes as dimers. These Gr channels allow single-file diffusion cations, thereby disrupting the usual ionic balance biological cells and inducing lysis. The microbicidal activity using different carriers such bilayer vesicles or disks, supported bilayers on silica, polystyrene nanoparticles has been described. antimicrobial was found depend strongly its formulation. Preliminary description self-assembled (Gr NPs) by our group showed superior performance for these nanospheres. In this work, we further characterize colloidal dispersions aqueous solution over range micromolar concentrations from turbidimetry, obedience Rayleigh law light scattered NPs smaller than wavelength incident light, dynamic scattering ascertain reproducibility physical characteristics NPs, effects viability five mammalian lines culture (0.5-5.0 μM). Thereby, differential cytotoxicity inferred comparison between microbial viability. results suggest that simple efficacious formulation obtained directly self-assembly deserves be exploited, aiming at systemic biomedical uses vivo against infectious diseases cancers.

Language: Английский

Citations

0
Antimicrobial coatings from gramicidin D nanoparticles and polymers DOI Creative Commons

Livia Cestaro de Souza Camargo,

Bianca Reche Bazan,

Rodrigo Tadeu Ribeiro

et al.

RSC Pharmaceutics, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Broad-spectrum, microbicidal, high-performance, transparent and washable coatings are made from gramicidin D nanoparticles hydrophilic polymer such as poly(diallyl dimethyl ammonium chloride) cast on or hydrophobic surfaces.

Language: Английский

Citations

1
Antimicrobial Peptides and Their Assemblies DOI Creative Commons
Ana Maria Carmona‐Ribeiro

Future Pharmacology, Journal Year: 2023, Volume and Issue: 3(4), P. 763 - 788

Published: Oct. 19, 2023

Antibiotic resistance requires alternatives to fight multi-drug resistant strains. Antimicrobial peptides (AMPs) act by disrupting or solubilizing microbial cell walls membranes in accordance with mechanisms difficult counteract from the microbe’s point of view. In this review, structure–activity relationships for AMPs and their assemblies are discussed, considering not only self-assembly but also interactions carriers optimal delivery combinations other complementary antimicrobials moieties covalently bound chemical structure. The effect formulations on AMP activity is evaluated, revealing a myriad possibilities. Depending interaction forces between AMP, carrier, elements added formulations, can be reduced, enhanced, remain unaffected. Approaches protecting against proteolysis may reduce activity.

Language: Английский

Citations

3