Preliminary Identification and Quantification of Individual Polyphenols in Fallopia japonica Plants and Honey and Their Influence on Antimicrobial and Antibiofilm Activities

Plants, Journal Year: 2024, Volume and Issue: 13(13), P. 1883 - 1883

Published: July 8, 2024

Fallopia japonica (FJ), an invasive plant species known for its rich bioactive compounds, has been used centuries in traditional Chinese medicine. Despite significant beekeeping potential, this aspect of FJ remains underexplored. This research aims to investigate the antimicrobial and antibiofilm properties plants honey. Notably, study is first identify individual phenolic compounds both tissues honey, highlighting resveratrol as a marker The tested inhibitory activity against seven bacterial strains: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Salmonella enteritidis, yeast Candida albicans. Disk diffusion microdilution methods were assess activity, while crystal violet staining test evaluated activity. Results showed that honey exhibited strong inhibition, particularly Gram-negative strains. most inhibition biofilm formation, by was observed aureus coli. A positive correlation found between polyphenols, especially resveratrol. antibacterial potential suggests promising applications sustainable beekeeping. Further necessary evaluate their health effects.

Language: Английский

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Rutin/Sulfobutylether-β-Cyclodextrin as a Promising Therapeutic Formulation for Ocular Infection

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(2), P. 233 - 233

Published: Feb. 5, 2024

Ocular pathologies present significant challenges to achieving effective therapeutic results due various anatomical and physiological barriers. Natural products such as flavonoids, alone or in association with allopathic drugs, many actions including anticancer, anti-inflammatory, antibacterial action. However, their clinical employment is challenging for scientists low water solubility. In this study, we designed a liquid formulation based on rutin/sulfobutylether-β-cyclodextrin (RTN/SBE-β-CD) inclusion complex treating ocular infections. The correct stoichiometry the accurate binding constant were determined by employing SupraFit software (2.5.120) UV-vis titration experiment. A deep physical–chemical characterization of RTN/SBE-β-CD was also performed; it confirmed predominant formation stable (Kc, 9660 M−1) 1:1 molar ratio, high solubility that 20 times (2.5 mg/mL) higher than free molecule (0.125 mg/mL), permitting dissolution solid within 30 min. NMR studies revealed involvement bicyclic flavonoid moiety complexation, which molecular modeling studies. vitro, antibiofilm activity assayed against Staphylococcus aureus Pseudomonas aeruginosa strains. demonstrated molecules.

Language: Английский

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In-vitro synergistic interactions of Baicalein in combination with β-lactam antibiotics against Methicillin-resistant Staphylococcus aureus

Exploratory Animal and Medical Research, Journal Year: 2024, Volume and Issue: 14(1), P. 44 - 49

Published: June 30, 2024

INTRODUCTIONStaphylococcus aureus (S. aureus) is the most prevalent bacterial organism which causes severe form of cow mastitis and poses a significant challenge to clean milk production [1].S. economic losses in terms sharp drop revenue, elimination superior animals, higher veterinary care expenses, need replace tainted [2,3].Even though antibiotics are cornerstone treatment for S. mastitis, excessive use proven antimicrobial agents management has culminated dramatic rise number antibiotic-resistant

Language: Английский

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Impact of Biofilms on Surface Properties of Polymethyl Methacrylate (PMMA) Resins

Journal of Basic Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Poly(methyl methacrylate) (PMMA) resins are widely used in medical and dental applications. Their susceptibility to bacterial biofilm formation poses significant challenges related material degradation infection risk. This study investigated the effects of Pseudomonas aeruginosa (P. aeruginosa) Staphylococcus aureus (S. aureus) biofilms on PMMA resin surface properties over a 45-day period at 35°C. The examined various parameters including adhesion, morphology, roughness, hydrophobicity, solid fraction, zeta potential. specimens were inoculated with bacteria incubated for 45 days. Biofilm adhesion was visually assessed, while characterization conducted using scanning electron microscopy (SEM), atomic force (AFM), roughness analysis, contact angle measurements, fraction determination, potential analysis. P. S. isolates selected based their biofilm-positive characteristics, which further confirmed Congo red assays through crystal violet staining spectrophotometric results demonstrated robust surfaces. SEM AFM imaging revealed textured surfaces elevated structures depressions within matrix. Biofilm-exposed exhibited significantly increased (Ra = 164.5 nm, Rq 169.5 nm) hydrophobicity (mean 85.5°-90.5°) compared control samples 38-50 55°). Solid measurements indicated denser matrix exposed (0.908) controls (0.65). Additionally, values more negative biofilm-exposed -84.2 mV) than (-45.0 mV). These findings underscore substantial alterations induced by biofilms, emphasizing critical need strategies prevent mitigate associated risks healthcare settings. Future research should focus developing anti-biofilm coatings or treatments preserve integrity functionality materials.

Language: Английский

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Nanostructured silver vanadate gel: evaluation of physicochemical, mechanical, and antibiofilm properties against a five-species oral model

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 102, P. 106366 - 106366

Published: Nov. 1, 2024

Language: Английский

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