Eicosapentaenoic Acid Triggers Phosphatidylserine Externalization in the Erythrocyte Membrane through Calcium Signaling and Anticholinesterase Activity DOI Creative Commons

Feryal H. Alharthy,

Jawaher Alsughayyir,

MA Alfhili

et al.

Physiological Research, Journal Year: 2024, Volume and Issue: unknown, P. 1075 - 1084

Published: Dec. 31, 2024

Hemolysis and eryptosis contribute to anemia encountered in patients undergoing chemotherapy. Eicosapentaenoic acid (EPA) is an omega-3 dietary fatty that has anticancer potential by inducing apoptosis cancer cells, but its effect on the physiology lifespan of red blood cells (RBCs) understudied. Human RBCs were exposed concentrations EPA (10–100 μM) for 24 h at 37 °C. Acetylcholinesterase (AChE) activity hemolysis measured colorimetric assays whereas annexin-V-FITC forward scatter (FSC) employed identify eryptotic cells. Oxidative stress was assessed H2DCFDA intracellular Ca2+ Fluo4/AM. significantly increased K+ leakage, LDH AST activities supernatants a concentration-dependent manner. also annexin-V-FITC-positive Fluo4 fluorescence decreased FSC AChE activity. A significant reduction hemolytic noted presence extracellular isosmotic urea, 125 mM KCl, polyethylene glycol 8000 (PEG 8000), not sucrose. In conclusion, stimulates through buildup inhibition. Urea, blocking KCl efflux, PEG alleviate EPA. The may be optimized using channel blockers chelators minimize toxicity off-target tissue.

Language: Английский

Diversity of Color, Infrared Spectra, and Phenolic Profile Correlation in Citrus Fruit Peels DOI Creative Commons
Rosa Zapata, Lorena Martínez‐Zamora, Francisco Artés‐Hernández

et al.

Published: Feb. 7, 2025

Language: Английский

Citations

0
Ginsenoside Rh2 Regulates the Calcium/ROS/CK1α/MLKL Pathway to Promote Premature Eryptosis and Hemolysis in Red Blood Cells DOI

Sumiah A. Alghareeb,

Jawaher Alsughayyir, Mohammad A. Alfhili

et al.

Toxicologic Pathology, Journal Year: 2024, Volume and Issue: 52(5), P. 284 - 294

Published: July 1, 2024

Ginsenoside Rh2 (GRh2) exhibits significant potential as an anticancer agent; however, progress in developing chemotherapeutic drugs is impeded by their toxicity toward off-target tissues. Specifically, anemia caused chemotherapy a debilitating side effect and can be red blood cell (RBC) hemolysis eryptosis. Cells were exposed to GRh2 the antitumor range hemolytic eryptotic markers examined under different experimental conditions using photometric cytofluorimetric methods. Ca

Language: Английский

Citations

1
Effect of hydrocortisone on the course of leprosic infection in an experiment DOI Open Access

G. N. Gennatulina,

L. V. Saroyants

Siberian Journal of Clinical and Experimental Medicine, Journal Year: 2024, Volume and Issue: 39(3), P. 144 - 149

Published: Oct. 2, 2024

Leprosy is a chronic infectious disease caused by Mycobacterium leprae (M. leprae), accompanied violation of various parts the immune system. In this regard, development an experimental model leprosy may be based on creation artificial defect macrophage link, in particular, using glucocorticoids. Aim: To develop and characterize generalized infection mice against background prolonged administration hydrocortisone. Material methods. conduct study, following groups were formed: control group – 50 infected Shepard method; similarly hydrocortisone administration. Hydrocortisone was administered intramuscularly single daily dose 25 mg/kg first day 2 hours before with M. suspension, then form four courses weeks at two-week intervals. Results. The led to acceleration reproduction mycobacteria paw pad compared (without hydrocortisone). generalization confirmed presence lepromatous structures granulomas from macrophages high content liver, spleen, lungs kidneys. Conclusion. This allows us study properties pathogen mechanisms pathogenesis disease, screen pharmacological drugs potential anti-leprosy activity future.

Language: Английский

Citations

0
Linolenic acid stimulates eryptosis and hemolysis through oxidative stress and CK1α/MLKL: protective role of melatonin, urea, and polyethylene glycol DOI

Feryal H. Alharthy,

Jawaher Alsughayyir, Mohammad A. Alfhili

et al.

Drug and Chemical Toxicology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 11

Published: Oct. 31, 2024

Anticancer medications cause anemia in patients through ill-defined mechanisms, including hemolysis and eryptosis. Although α-linolenic acid (ALA) possesses anticancer properties against a variety of cancer cells, there is dearth evidence regarding how it modulates red blood cell (RBC) physiology. RBCs from healthy donors were subjected to concentrations ALA (2.5, 5, 10, 20, 40, 80, 100 μM) at 37 °C for 24 h, colorimetric tests used determine acetylcholinesterase (AChE) activity. Meanwhile, flow cytometry was employed identify eryptotic cells using annexin-V-FITC forward scatter (FSC), Fluo4/AM detect Ca2+, H2DCFDA assess oxidative stress. significantly increased eryptosis concentration-dependent manner, along with elevated Fluo4 DCF fluorescence, erythrocyte sedimentation rate, reduced FSC AChE Moreover, the addition D4476, necrosulfonamide, melatonin, isosmotic urea, polyethylene glycol 8000 – but not sucrose inhibited toxicity. In conclusion, stimulates Ca2+ buildup, stress, anticholinesterase activity, casein kinase 1α (CK1α), mixed lineage domain-like protein (MLKL). The activity may be potentiated by use channel blockers chelators, antioxidants, CK1α MLKL inhibitors ameliorate its toxicity RBCs.

Language: Английский

Citations

0
Docosahexaenoic Acid Promotes Eryptosis and Haemolysis through Oxidative Stress/Calcium/Rac1 GTPase Signalling DOI

Feryal H. Alharthy,

Jawaher Alsughayyir, Mohammad A. Alfhili

et al.

Folia Biologica, Journal Year: 2024, Volume and Issue: 70(3), P. 179 - 188

Published: Jan. 1, 2024

Docosahexaenoic acid (DHA) is an omega-3 polyunsaturated fatty with promising anticancer potential. Anaemia a frequent adverse effect of treatment caused in part by eryptosis and haemolysis. Thus, it important to investigate the role DHA red blood cell (RBC) death. RBCs were treated concentrations (10–100 μM) under different physiological conditions, fluorescence-assisted sorting was employed measure eryptotic markers. Cell membrane scrambling detected annexin-V-FITC labelling, cytoplasmic Ca 2+ Fluo4/AM, size forward scatter (FSC), oxidative stress H 2 DCFDA. Haemolytic markers also assayed photometric methods. significant phospholipid accumulation, loss cellular volume, stress. These changes associated dacrocyte formation, as revealed electron microscopy. Moreover, exhibited dual on integrity: haemolytic isotonic conditions anti-haemolytic hypotonic environments. Importantly, inhibition Rac1 GTPase activity NSC23766 significantly reduced DHA-mediated haemolysis, did co-administration either sucrose or polyethylene glycol 8,000. Conversely, presence 125 mM KCl urea without extracellular exacerbated toxicity. In conclusion, this first report that identifies key biochemical mechanisms underlying cytotoxic effects RBCs, promoting further development validation therapy.

Language: Английский

Citations

0
Eicosapentaenoic Acid Triggers Phosphatidylserine Externalization in the Erythrocyte Membrane through Calcium Signaling and Anticholinesterase Activity DOI Creative Commons

Feryal H. Alharthy,

Jawaher Alsughayyir,

MA Alfhili

et al.

Physiological Research, Journal Year: 2024, Volume and Issue: unknown, P. 1075 - 1084

Published: Dec. 31, 2024

Hemolysis and eryptosis contribute to anemia encountered in patients undergoing chemotherapy. Eicosapentaenoic acid (EPA) is an omega-3 dietary fatty that has anticancer potential by inducing apoptosis cancer cells, but its effect on the physiology lifespan of red blood cells (RBCs) understudied. Human RBCs were exposed concentrations EPA (10–100 μM) for 24 h at 37 °C. Acetylcholinesterase (AChE) activity hemolysis measured colorimetric assays whereas annexin-V-FITC forward scatter (FSC) employed identify eryptotic cells. Oxidative stress was assessed H2DCFDA intracellular Ca2+ Fluo4/AM. significantly increased K+ leakage, LDH AST activities supernatants a concentration-dependent manner. also annexin-V-FITC-positive Fluo4 fluorescence decreased FSC AChE activity. A significant reduction hemolytic noted presence extracellular isosmotic urea, 125 mM KCl, polyethylene glycol 8000 (PEG 8000), not sucrose. In conclusion, stimulates through buildup inhibition. Urea, blocking KCl efflux, PEG alleviate EPA. The may be optimized using channel blockers chelators minimize toxicity off-target tissue.

Language: Английский

Citations

0