Enhanced Antibacterial Efficacy of Bioceramic Implants Functionalized with Ciprofloxacin: An In Silico and In Vitro Study DOI Creative Commons

Renata-Maria Văruț,

Luciana Teodora Rotaru, Diana Cimpoeşu

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(8), P. 998 - 998

Published: July 28, 2024

This study explores the antibacterial efficacy and cytotoxicity of ciprofloxacin-functionalized bioceramic implants. We synthesized hydroxyapatite-ciprofloxacin (HACPXCS) composites applied them to titanium substrates (Ti-HA-CPX), evaluating their performance in vitro against Staphylococcus aureus (ATCC 25923) Escherichia coli 25922). Antibacterial activity was assessed using Kirby-Bauer disc diffusion method, while tested mesenchymal stem cells. The results demonstrated that Ti-HA-CPX exhibited superior activity, with inhibition zones 33.5 mm (MIC 0.5 µg/mL) for 27.5 0.25 However, showed moderate (80% cell viability). HACPXCS composites, whether chemically or mechanically mixed (HACPXMM), also displayed significant but ranging from low levels. Molecular docking studies confirmed strong binding affinities between ciprofloxacin bacterial proteins, correlating enhanced efficacy. These findings suggest offer a promising approach single-stage surgical interventions treating chronic osteomyelitis infected fractures, balancing effectiveness manageable cytotoxicity.

Language: Английский

Gelatin-based nanoparticles and antibiotics: a new therapeutic approach for osteomyelitis? DOI Creative Commons

Ali Sherafati Chaleshtori,

Zeynab Marzhoseyni,

Nikoo Saeedi

et al.

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11

Published: July 30, 2024

The result of infection bone with microorganisms is osteomyelitis and septic arthritis. Methicillin-resistant Staphylococcus aureus (MRSA) responsible for most its cases (more than 50%). Since MRSA resistant to many treatments, it accompanied by high costs numerous complications, necessitating more effective new treatments. Recently, development gelatin nanoparticles have attracted the attention scientists biomedicine itself, been utilized as a delivery vehicle antibiotics because their biocompatibility, biodegradability, cost-effectiveness. Promising results reported modification combinations chemical agents. Although these findings suggested that has potential be suitable option continuous release in arthritis treatment, they still not become routine clinical practices. deliver antibiotic using gelatin-derived composites vancomycin which showed good efficacy. To date, number pre-clinical studies evaluated utility gelatin-based management osteomyelitis. Gelatin-based were found satisfactory performance control infection, well promotion defect repair chronic models. This review summarized available evidence provides insight into controlled antibiotics.

Language: Английский

Citations

4
Progress in the role of nanoparticles in the diagnosis and treatment of bone and joint tuberculosis DOI Creative Commons

Yitong Ding,

B P Li,

Yangfei Yi

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 24, 2025

Bone and joint tuberculosis (BJTB), caused by Mycobacterium (MTB), is a prevalent form of extrapulmonary that poses significant challenges to global public health due difficulties in early diagnosis, prolonged treatment cycles, drug resistance. Recent advancements nanotechnology have introduced novel solutions for the detection precise BJTB, leveraging unique physicochemical properties such as high specific surface area, targeted delivery capabilities, sustained release, excellent biocompatibility. In diagnostic applications, nanomaterials markedly enhance sensitivity accuracy methods while reducing testing time. These technologies are adaptable resource-limited settings, enabling earlier patient intervention mitigating disease progression risk. therapeutic prolong retention bone tissue through delivery, thereby decreasing medication frequency minimizing toxic side effects, which significantly improves efficacy. Despite substantial progress, further research required address long-term safety concerns, broaden clinical applicability, evaluate performance under complex pathological conditions. This review summarizes recent diagnosing treating BJTB identifies key areas future research, laying groundwork advancing precision medicine personalized treatments.

Language: Английский

Citations

0
Advances in spray drying technology for anti-tubercular formulation development: A comprehensive review DOI
Eknath Kole, Krishna Jadhav, Yogesh A. Sonar

et al.

Drying Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 37

Published: Jan. 27, 2025

Language: Английский

Citations

0
Enhanced Antibacterial Efficacy of Bioceramic Implants Functionalized with Ciprofloxacin: An In Silico and In Vitro Study DOI Creative Commons

Renata-Maria Văruț,

Luciana Teodora Rotaru, Diana Cimpoeşu

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(8), P. 998 - 998

Published: July 28, 2024

This study explores the antibacterial efficacy and cytotoxicity of ciprofloxacin-functionalized bioceramic implants. We synthesized hydroxyapatite-ciprofloxacin (HACPXCS) composites applied them to titanium substrates (Ti-HA-CPX), evaluating their performance in vitro against Staphylococcus aureus (ATCC 25923) Escherichia coli 25922). Antibacterial activity was assessed using Kirby-Bauer disc diffusion method, while tested mesenchymal stem cells. The results demonstrated that Ti-HA-CPX exhibited superior activity, with inhibition zones 33.5 mm (MIC 0.5 µg/mL) for 27.5 0.25 However, showed moderate (80% cell viability). HACPXCS composites, whether chemically or mechanically mixed (HACPXMM), also displayed significant but ranging from low levels. Molecular docking studies confirmed strong binding affinities between ciprofloxacin bacterial proteins, correlating enhanced efficacy. These findings suggest offer a promising approach single-stage surgical interventions treating chronic osteomyelitis infected fractures, balancing effectiveness manageable cytotoxicity.

Language: Английский

Citations

0