Harnessing the potential of deep eutectic solvents in biocatalysis: design strategies using CO2 to formate reduction as a case study DOI Creative Commons
Marijan Logarušić,

Karla Šubar,

Maja Nikolić

et al.

Frontiers in Chemistry, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 25, 2024

Deep eutectic solvents (DESs) have emerged as green with versatile applications, demonstrating significant potential in biocatalysis. They often increase the solubility of poorly water-soluble substrates, serve smart co-substrates, modulate enzyme stereoselectivity, and potentially improve activity stability. Despite these advantages, screening for an optimal DES determining appropriate water content a given biocatalytic reaction remains complex time-consuming process, posing challenge.

Language: Английский

Exploring the Potential of PEG-Based Deep Eutectic Solvents as a Sustainable Alternative for Extraction of Biological Macromolecules Bovine Serum Hemoglobin DOI Creative Commons
M. S. Siddiqui, Md. Sayem Alam, Maroof Ali

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(7), P. 6839 - 6856

Published: Feb. 14, 2025

In recent years, deep eutectic solvents (DESs) have garnered significant attention as promising green alternatives to conventional organic for a wide range of applications. this study, four novel polyethylene glycol (PEG)-based DESs were prepared and evaluated their physicochemical properties, including density, dynamic viscosity, kinematic viscosity. Fourier transform infrared spectroscopy (FT-IR) NMR analyses revealed substantial intermolecular interactions between the hydrogen bond donor acceptor components, confirming formation stable DES systems. The application was tested in biological separation, specifically selective extraction bovine serum hemoglobin (BHb). This study demonstrates efficacy PEG-based selectively extracting BHb. Among studied, DES-4 (PEG-600) achieved highest efficiency 88%, while maintaining protein stability. Spectroscopic techniques, UV–visible, fluorescence, light scattering, circular dichroism, FT-IR, employed investigate mechanism, conformational changes structure, DES-protein interactions. These methods provided insights into structural stability functionality BHb during process. characterizations confirmed unique properties DESs, making them viable candidates sustainable extraction. Their compatibility, excellent efficiency, short separation times underscore potential environmentally friendly long-lasting substitutes techniques. highlights advancement chemistry biotechnological applications, offering an efficient platform integrity.

Language: Английский

Citations

0
Enzymatic Oxidation of Hydroxytyrosol in Deep Eutectic Solvents for Chitosan Functionalization and Preparation of Bioactive Nanogels DOI Open Access
Myrto G. Bellou, Anastasia Skonta, Alexandra V. Chatzikonstantinou

et al.

Catalysts, Journal Year: 2025, Volume and Issue: 15(2), P. 180 - 180

Published: Feb. 14, 2025

Biocatalytic processes for the formation of bioactive compounds and biopolymer preparations that can be applied in pharmaceuticals cosmetics are gaining increasing interest due to their safety sustainability, relying on environmentally friendly approaches biocompatible compounds. In this work, we investigate implementation various Deep Eutectic Solvents (DES) laccase-catalyzed oxidation hydroxytyrosol (HT), aiming produce its oligomer derivatives such as HT dimer trimer. The composition reaction mixture which oligomers’ yield was highest 70% v/v Bet:PG (1:4 molar ratio). oligomers formed were subsequently used non-enzymatic grafting chitosan (CS) development chitosan-based nanogels (NG). Grafted prepared by ionic gelation using sodium tripolyphosphate (TPP) a cross-linking agent. functionalized characterized Fourier-Transform Infrared (FTIR) Nuclear Magnetic Resonance (NMR) spectroscopy, while Scanning Electron Microscopy (SEM) employed nanogel characterization. Compared unmodified nanogels, grafted exhibited almost ten-fold higher antioxidant activity approximately 20% greater antibacterial activity.

Language: Английский

Citations

0
The Role of Natural Deep Eutectic Solvents in a Hydrogel Formulation Containing Lidocaine DOI Creative Commons

Feria Hasanpour,

Mária Budai-Szűcs, Anita Kovács

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 324 - 324

Published: March 2, 2025

Background/Objectives: This study investigates the use of natural deep eutectic solvents (NADESs) in enhancing solubility and skin permeation a lidocaine base, lipophilic form, hydrogel systems. The aim was to develop an environmentally sustainable biocompatible alternative conventional formulations, improving dermal therapeutic efficacy. Methods: base dissolved hydrophilic NADES system composed choline chloride citric acid, facilitating enhanced solubility, likely through new molecular interactions. Then, pH-adjusted hydrogels were formulated optimized by employing 32 full factorial design. Raman nuclear magnetic resonance (NMR) spectroscopy applied evaluate stability optimal formulation. biopharmaceutical properties investigated using vitro drug release studies. In vivo tests assessed physiological parameters such as hydration transepidermal water loss. Results: developed NADES-containing significantly improved lidocaine. Skin studies demonstrated compared with ointment. These improvements, namely formulation its increased permeation, attributed dual effect NADES. Conclusions: represent promising green technology for formulating lidocaine-containing preparations. approach offers biocompatible, natural-based that can enhance bioavailability efficacy topical anesthetics.

Language: Английский

Citations

0
Strategies to Prepare Chitin and Chitosan-Based Bioactive Structures Aided by Deep Eutectic Solvents: A Review DOI Open Access

D. Alonzo Durante-Salmerón,

Isabel Fraile-Gutiérrez, Rubén Gil-Gonzalo

et al.

Catalysts, Journal Year: 2024, Volume and Issue: 14(6), P. 371 - 371

Published: June 10, 2024

Chitin and chitosan, abundant biopolymers derived from the shells of crustaceans cell walls fungi, have garnered considerable attention in pharmaceutical circles due to their biocompatibility, biodegradability, versatile properties. Deep eutectic solvents (DESs), emerging green composed mixtures hydrogen bond acceptors donors, offer promising avenues for enhancing solubility functionality chitin chitosan formulations. This review delves into potential utilizing DESs as highlighting efficiency dissolving these polymers, which facilitates production novel drug delivery systems, wound dressings, tissue engineering scaffolds, antimicrobial agents. The distinctive physicochemical properties DESs, including low toxicity, volatility, adaptable solvation power, enable customization chitosan-based materials meet specific requirements. Moreover, environmentally friendly nature aligns with growing demand sustainable eco-friendly processes manufacturing. revision underscores recent advances illustrating role evolving applications laying groundwork development innovative systems biomedical enhanced efficacy safety profiles.

Language: Английский

Citations

2
Harnessing the potential of deep eutectic solvents in biocatalysis: design strategies using CO2 to formate reduction as a case study DOI Creative Commons
Marijan Logarušić,

Karla Šubar,

Maja Nikolić

et al.

Frontiers in Chemistry, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 25, 2024

Deep eutectic solvents (DESs) have emerged as green with versatile applications, demonstrating significant potential in biocatalysis. They often increase the solubility of poorly water-soluble substrates, serve smart co-substrates, modulate enzyme stereoselectivity, and potentially improve activity stability. Despite these advantages, screening for an optimal DES determining appropriate water content a given biocatalytic reaction remains complex time-consuming process, posing challenge.

Language: Английский

Citations

2