Prospecting Pharmacologically Active Biocompounds from the Amazon Rainforest: In Vitro Approaches, Mechanisms of Action Based on Chemical Structure, and Perspectives on Human Therapeutic Use
Pharmaceuticals,
Journal Year:
2024,
Volume and Issue:
17(11), P. 1449 - 1449
Published: Oct. 30, 2024
The
Amazon
rainforest
is
an
important
reservoir
of
biodiversity,
offering
vast
potential
for
the
discovery
new
bioactive
compounds
from
plants.
In
vitro
studies
allow
investigation
biological
processes
and
interventions
in
a
controlled
manner,
making
them
fundamental
pharmacological
biotechnological
research.
These
approaches
are
faster
less
costly
than
vivo
studies,
providing
standardized
conditions
that
enhance
reproducibility
precision
data.
However,
methods
have
limitations,
including
inability
to
fully
replicate
complexity
living
organism
absence
complete
physiological
context.
Translating
results
models
not
always
straightforward,
due
differences
pharmacokinetics
interactions.
this
context,
aim
literature
review
assess
advantages
disadvantages
search
drugs
Amazon,
identifying
challenges
limitations
associated
with
these
comparing
testing.
Thus,
bioprospecting
involves
evaluating
plant
extracts
through
bioassays
investigate
pharmacological,
antimicrobial,
anticancer
activities.
Phenolic
terpenes
frequently
identified
as
main
agents,
exhibiting
antioxidant,
anti-inflammatory,
antineoplastic
Chemical
characterization,
molecular
modifications,
development
delivery
systems,
such
nanoparticles,
highlighted
improve
therapeutic
efficacy.
Therefore,
offers
great
drugs;
however,
significant
challenges,
standardization
extraction
need
clinical
trials,
must
be
overcome
become
viable
medications.
Language: Английский
Optimization of a Pressurized Extraction Process Based on a Ternary Solvent System for the Recovery of Neuroprotective Compounds from Eucalyptus marginata Leaves
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
26(1), P. 94 - 94
Published: Dec. 26, 2024
Green
chemistry
focuses
on
reducing
the
environmental
impacts
of
chemicals
through
sustainable
practices.
Traditional
methods
for
extracting
bioactive
compounds
from
Eucalyptus
marginata
leaves,
such
as
hydro-distillation
and
organic
solvent
extraction,
have
limitations,
including
long
extraction
times,
high
energy
consumption,
potential
toxic
residues.
This
study
explored
use
supercritical
fluid
(SFE),
pressurized
liquid
(PLE),
gas-expanded
(GXL)
processes
to
improve
efficiency
selectivity.
These
techniques
were
combined
in
a
single
mixture
design,
where
CO2
was
used
experiments
carried
out
under
SFE,
while
water
ethanol
PLE
GXL
by
varying
concentration
solvents
cover
all
possibilities.
The
neuroprotective
activity
extracts
evaluated
measuring
their
antioxidant,
anti-inflammatory,
acetylcholinesterase
inhibition
properties.
optimization
resulted
novel
with
an
optimal
ternary
27%
CO2,
55%
ethanol,
18%
water,
degree
desirability
(R2
=
88.59%).
Chromatographic
analysis
GC-MS
HPLC-ESI-MS/MS
identified
over
49
metabolites.
designed
process
offers
promising
approach
producing
phenolic-rich
plant
industrial
applications.
Language: Английский