Seasonal Variations in Chemical Composition and Antibacterial and Antioxidant Activities of Rosmarinus officinalis L. Essential Oil from Southwestern Romania

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(2), P. 681 - 681

Published: Jan. 11, 2025

Our study reports for the first time, over a 12-month period, seasonal variations in chemical composition and antibacterial antioxidant activity of Rosmarinus officinalis L. essential oil (RoEO) from Southwestern Romania (Oltenia region). To analyze constituents RoEO, comprehensive gas chromatography/mass spectrometry (GC/MS) method was employed. The analysis aimed to identify quantify various components by comparing their mass spectra with reference National Institute Standards Technology (NIST) Library 2020. minimum inhibitory concentration (MIC) values Staphylococcus aureus were determined using microdilution (96-well plates). analyzed 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H2O2) radical scavenging assays. This provided detailed profile RoEO’s constituents, revealing significant monthly variations. Key compounds, such as camphor, eucalyptol, α-pinene, camphene, α-myrcene, quantified, alongside lesser-studied like β-pinene, α-terpinene, linalool, terpinolene, carvacrol. Comparisons made sample Tunisia. Oxygenated monoterpenes reach highest (56.82–66.94%), followed monoterpene hydrocarbons (30.06–40.28%), sesquiterpene (0.90–2.44%), oxygenated sesquiterpenes (0.02–0.23%). Camphor found high concentrations ranging 29.41% 40.03%. 1,8-Cineole another dominant compound, 13.07% 16.16%, significantly lower compared Tunisian (52.77%). α-Pinene ranged 11.36% 19.33%, while α-myrcene 1.65% 3.08%. Correlations between specific compounds bioactivity explored understand contributions overall efficacy RoEO. provides valuable insights into potential applications variability RoEO Romania.

Language: Английский

Antiurolithic activity of vanillin in ethylene glycol-induced hyperoxaluric rat model

Urolithiasis, Journal Year: 2025, Volume and Issue: 53(1)

Published: March 21, 2025

Language: Английский

Prolonged Diuretic, Natriuretic, and Potassium- and Calcium-Sparing Effect of Hesperidin in Hypertensive Rats

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1324 - 1324

Published: April 27, 2025

Systemic hypertension is a major global health concern, significantly contributing to the risk of cardiovascular, cerebrovascular, and renal diseases. Antihypertensive medications play crucial role in lowering blood pressure, with diuretics serving as particularly effective first-line therapy. However, development new compounds diuretic properties, protective effects, unique mechanisms action remains critical area research for improving clinical outcomes. In this context, present study investigated potential citrus flavonoid hesperidin rats. Male spontaneously hypertensive normotensive rats were treated at dose 3.0 mg/kg daily seven days. Urine samples analyzed electrolytes (Na+, K+, Cl-, Ca2+), biochemical parameters, crystal precipitation, while tissues examined histologically. Hesperidin treatment resulted significant natriuretic along potassium- calcium-sparing properties. Furthermore, marked reduction calcium oxalate formation was observed hesperidin-treated group. Histological analysis indicated effect on tissue, structural preservation Docking studies revealed that hesperetin, active metabolite formed upon oral administration, exhibited high binding affinity calcium-sensing receptor (CaSR). This hypothesis may explain its preventing urinary crystalluria effects.

Language: Английский