Preclinical and Molecular Docking Insights into the Chemopreventive Role of Fenugreek Seed Extract in a Murine Model of Colorectal Cancer DOI Creative Commons
Arif Khan, Khaled S. Allemailem,

Arwa Essa Alradhi

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 490 - 490

Published: March 28, 2025

Background/Objectives: Colorectal cancer (CRC) remains a leading cause of cancer-related mortality, necessitating the development effective preventive strategies. Fenugreek (Trigonella foenum-graecum) possesses well-documented pharmacological properties; however, its chemopreventive potential in colorectal unexplored. This study evaluates efficacy methanolic fenugreek seed extract (FSE) an azoxymethane (AOM)-induced murine model, focusing on modulation oxidative stress, regulation biomarkers, induction apoptosis, and maintenance epithelial integrity. Methods: FSE was extracted using cold maceration (yield: 24%) analyzed by gas chromatography–mass spectrometry (GC-MS), identifying 13 bioactive compounds, including benzene, 1,3-dimethyl-; 1,3-cyclopentadiene, 5-(1-methylethylidene)-; o-Xylene; benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-; 1,2,3-trimethyl-. All compounds identified were matched with NIST library high confidence. Molecular docking used to assess interactions bioactives E-cadherin–β-catenin complexes. Swiss albino mice received pre-treatment before AOM continued this treatment three times weekly for 21 weeks. Key assessments included survival analysis, body weight changes, serum biomarker levels (GGT, 5′-NT, LDH), antioxidant enzyme activities (SOD, CAT, GPx1, MDA), reactive oxygen species (ROS) quantification, apoptosis detection via flow cytometry, immunofluorescence-based evaluation E-cadherin dynamics. Results: improved rates, mitigated AOM-induced loss, dose-dependently reduced levels. Antioxidant activity restored, while MDA declined. A dose-dependent increase ROS facilitated as confirmed cytometry (16.7% low-dose group 34.5% high-dose group). Immunofluorescence studies revealed that FSE-mediated restoration localization counteracted disruptions. Conclusions: exhibits potent against CRC modulating regulating key inducing restoring These findings support further investigations into clinical relevance prevention.

Language: Английский

Preclinical and Molecular Docking Insights into the Chemopreventive Role of Fenugreek Seed Extract in a Murine Model of Colorectal Cancer DOI Creative Commons
Arif Khan, Khaled S. Allemailem,

Arwa Essa Alradhi

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 490 - 490

Published: March 28, 2025

Background/Objectives: Colorectal cancer (CRC) remains a leading cause of cancer-related mortality, necessitating the development effective preventive strategies. Fenugreek (Trigonella foenum-graecum) possesses well-documented pharmacological properties; however, its chemopreventive potential in colorectal unexplored. This study evaluates efficacy methanolic fenugreek seed extract (FSE) an azoxymethane (AOM)-induced murine model, focusing on modulation oxidative stress, regulation biomarkers, induction apoptosis, and maintenance epithelial integrity. Methods: FSE was extracted using cold maceration (yield: 24%) analyzed by gas chromatography–mass spectrometry (GC-MS), identifying 13 bioactive compounds, including benzene, 1,3-dimethyl-; 1,3-cyclopentadiene, 5-(1-methylethylidene)-; o-Xylene; benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-; 1,2,3-trimethyl-. All compounds identified were matched with NIST library high confidence. Molecular docking used to assess interactions bioactives E-cadherin–β-catenin complexes. Swiss albino mice received pre-treatment before AOM continued this treatment three times weekly for 21 weeks. Key assessments included survival analysis, body weight changes, serum biomarker levels (GGT, 5′-NT, LDH), antioxidant enzyme activities (SOD, CAT, GPx1, MDA), reactive oxygen species (ROS) quantification, apoptosis detection via flow cytometry, immunofluorescence-based evaluation E-cadherin dynamics. Results: improved rates, mitigated AOM-induced loss, dose-dependently reduced levels. Antioxidant activity restored, while MDA declined. A dose-dependent increase ROS facilitated as confirmed cytometry (16.7% low-dose group 34.5% high-dose group). Immunofluorescence studies revealed that FSE-mediated restoration localization counteracted disruptions. Conclusions: exhibits potent against CRC modulating regulating key inducing restoring These findings support further investigations into clinical relevance prevention.

Language: Английский

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