Anti-Staphylococcus aureus Activity and Structural Characterization of Rationally Designed Peptides DOI Creative Commons
Lorenza Artesani, Mariana Gallo, Laura Giovati

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 437 - 437

Published: April 26, 2025

Background/Objectives: Microbial infections represent a significant threat to public health due the emergence and spread of antimicrobial resistance. Adjunctive alternative therapeutic strategies are explored tackle this issue, including use natural or synthetic peptides. Previous research showed that antibody-derived peptides possess antimicrobial, antiviral, immunomodulatory properties. This study aimed characterize newly designed evaluate their effectiveness against representative strains Staphylococcus aureus, drug-resistant isolates. Methods: Colony-forming unit assays confocal microscopy studies were performed peptide activity planktonic microbial cells. Cytotoxicity tests on THP-1 human monocytic Circular dichroism (CD) nuclear magnetic resonance (NMR) employed for conformational characterization Results: The half-maximal effective concentrations bacterial reference isolates ranged from 0.17 18.05 µM, while cytotoxic effects not observed mammalian A killing kinetics analysis observation by interaction between bacteria suggested mechanism action involving membrane perturbation. CD all predominantly exhibit random coil arrangement in aqueous solution. NMR spectroscopy revealed most active adopts helical conformation presence mimetics. Conclusions: structural evaluation peptides’ may lead selection candidate be further studied develop an treatment caused strains.

Language: Английский

Anti-Staphylococcus aureus Activity and Structural Characterization of Rationally Designed Peptides DOI Creative Commons
Lorenza Artesani, Mariana Gallo, Laura Giovati

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 437 - 437

Published: April 26, 2025

Background/Objectives: Microbial infections represent a significant threat to public health due the emergence and spread of antimicrobial resistance. Adjunctive alternative therapeutic strategies are explored tackle this issue, including use natural or synthetic peptides. Previous research showed that antibody-derived peptides possess antimicrobial, antiviral, immunomodulatory properties. This study aimed characterize newly designed evaluate their effectiveness against representative strains Staphylococcus aureus, drug-resistant isolates. Methods: Colony-forming unit assays confocal microscopy studies were performed peptide activity planktonic microbial cells. Cytotoxicity tests on THP-1 human monocytic Circular dichroism (CD) nuclear magnetic resonance (NMR) employed for conformational characterization Results: The half-maximal effective concentrations bacterial reference isolates ranged from 0.17 18.05 µM, while cytotoxic effects not observed mammalian A killing kinetics analysis observation by interaction between bacteria suggested mechanism action involving membrane perturbation. CD all predominantly exhibit random coil arrangement in aqueous solution. NMR spectroscopy revealed most active adopts helical conformation presence mimetics. Conclusions: structural evaluation peptides’ may lead selection candidate be further studied develop an treatment caused strains.

Language: Английский

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