Impact of nucleotide hydrophobicity on oligonucleotides separation in liquid chromatography

Journal of Chromatography A, Journal Year: 2025, Volume and Issue: 1753, P. 465968 - 465968

Published: April 14, 2025

We investigated the contribution of nucleotide (nt) type and modifications on retention resolution 22-24 nt long oligonucleotides in ion-pairing reversed-phase (IP RP) liquid chromatography (LC) hydrophilic interaction (HILIC) methods. The nucleotides relative hydrophobicity affects oligonucleotide LC separation selectivity. reagents higher (dipropylamine < dibutylamine dipentylamine) require a greater organic solvents concentration to elute IP RP LC. observed that solvent reduced (oligo)nucleotide led more uniform full-length from its truncated products. also diastereomeric 24 with single phosphorothioate modification at 5'-end. is nucleotide-motif dependent; G (and modified G) adjacent linkage yield isomers than other types HILIC.

Language: Английский

Bioinformatics guided synthesis of natural product cyclic peptides

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский