Scaffold for the Minimally Invasive Repair of Soft Tissue Inspired by Soggy Shrunk Puffed Food DOI Creative Commons
Yong He

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 4, 2023

Abstract Minimally invasive injection of tissue engineering scaffolds has been gaining attention due to featuring several advantages requiring a small incision, entailing simple operation procedure, and involving quick recovery. However, it remains challenge because larger must pass through an extremely thin needle. Herein, inspired by the phenomenon that puffed food becomes soggy shrinks when exposed air, novel scaffold treatment method is proposed; namely, lyophilization & dampening (L&D) treatment, which reduces printed hydrogel volume around 90%. Lyophilization can remove water inside scaffolds; Dampening is, placing freeze-dried into vapor atmosphere, make shrunk suitable size for minimally injection. Moreover, unlike existing air-dried technique feature high mechanical modulus, developed herein low modulus (2.88 kPa), thus minimizing foreign body sensation after implantation. Furthermore, injected rapidly swell their original act as regeneration media. Accordingly, specialized tool namely “BioGun” designed ("BioBullet”). This strategy would potentially overcome technical bottlenecks limiting clinical soft defect repair.

Language: Английский

Nano-fibrous biopolymers as building blocks for gel networks: Interactions, characterization, and applications DOI Creative Commons
Xiaohui Mao, Yujie Liu, Chenyu Qiao

et al.

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 338, P. 103398 - 103398

Published: Jan. 9, 2025

Biopolymers derived from natural resources are highly abundant, biodegradable, and biocompatible, making them promising candidates to replace non-renewable fossil fuels mitigate environmental health impacts. Nano-fibrous biopolymers possessing advantages of entangle with each other through inter-/intra-molecular interactions, serving as ideal building blocks for gel construction. These biopolymer nanofibers often synergize nano-building enhance gels desirable functions eco-friendliness across various applications in biomedical, environmental, energy sectors. The interactions directly affect the assembly blocks, which determines structure gels, integrity connected influencing mechanical properties performance specific applications. This review focuses on four (cellulose, chitin, silk, collagen), commonly used preparations, representatives polysaccharides polypeptides. covalent non-covalent between materials have been categorized discussed relation resulting network structures properties. Nanomechanical characterization techniques, such surface forces apparatus (SFA) atomic force microscopy (AFM), employed precisely quantify intermolecular blocks. these classified correlated their act "sewing threads", connecting all establish suitable functions. aims provide a comprehensive understanding involved preparation design principles needed achieve targeted functional gels.

Language: Английский

Citations

0
Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering DOI Creative Commons
Zahra Bashiri, Zahra Khosrowpour, Ali Moghaddaszadeh

et al.

Engineering in Life Sciences, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 1, 2025

ABSTRACT Recent interest has been focused on extracellular matrix (ECM)–based scaffolds totreat critical‐sized bone injuries. In this study, urea was used to decellularize and solubilize human placenta tissue. Then, different concentrations of ECM were composited with 8% alginate (Alg) 12% silk fibroin (SF) for printing in order produce a natural 3D construct that resembled The physical biological features the printed structures evaluated entirely vitro. Finally, rat model employed examine optimal scaffold (5% ECM) as transplant healing cranial lesions. present investigation demonstrated decellularizing placental tissue fragments led efficient removal cell debris. addition, remarkable improvement scaffolds' mechanical properties observed by increasing concentration. histology studies real‐time PCR results acceleration regeneration lesions treated 5%ECM‐SF/Alg at 4 8 weeks after implantation. Overall, these proved ECM‐printed could potentially biomimetic grafts reconstruct significant defects now promise proceed clinical studies.

Language: Английский

Citations

0
Improved alginate bio-ink by recombinant self-assembled cell-sized spider-silk inspired-biopolymer DOI

Dean W. Robinson,

Miriam Gubelbank,

Ella Sklan

et al.

Bioprinting, Journal Year: 2025, Volume and Issue: unknown, P. e00387 - e00387

Published: Jan. 1, 2025

Language: Английский

Citations

0
In vitro and ex vivo models of the oral mucosa as platforms for the validation of novel drug delivery systems DOI Creative Commons
Robyn A. Macartney,

A. K. Das,

Atina G Imaniyyah

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 1, 2025

The benefit of complex 3D models to facilitate the robust testing new drugs and drug delivery systems during developmental stages pharmaceutical manufacturing has recently become distinguished within field. Recognition this need by industry provided a motivation for research into development reliable use in delivery, biomaterials, tissue engineering. Both vitro ex vivo can enhance drug-testing discovery prospects over more traditionally used 2D, monolayer culture animal models. Despite widespread acceptance that modelling is advantageous field, there remains lack standardisation throughout literature. This article provides an extensive review current literature on vitro, oral mucosa applications; advantages, limitations, recommendations future improved application.

Language: Английский

Citations

0
Alginates in drug delivery DOI
Carlo Santulli

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 293 - 311

Published: Jan. 1, 2025

Citations

0
Kappa-carrageenan based hybrid hydrogel for soft tissue engineering applications DOI

Fereshteh Safarpour,

Mahshid Kharaziha,

H. Mokhtari

et al.

Biomedical Materials, Journal Year: 2023, Volume and Issue: 18(5), P. 055005 - 055005

Published: June 24, 2023

Biological materials such as cell-derived membrane vesicles have emerged alternative sources for molecular delivery systems, owing to multicomponent features, the inherent functionalities and signaling networks, easy-to-carry therapeutic agents with various properties. Herein, red blood cell (RBCM) vesicle-laden methacrylate kappa-carrageenan (KaMA) composite hydrogel is introduced soft tissue engineering. Results revealed that characteristics of hybrid hydrogels were significantly modulated by changing RBCM vesicle content. For instance, incorporation 20% (v/v) enhanced compressive strength from 103 ± 26 kPa 257 18 improved toughness under cyclic loading 1.0 0.4 kJ m-3to 4.0 0.5 m-3after 5thcycle. also used encapsulation curcumin (CUR) a hydrophobic drug molecule. showed controlled release CUR over three days immersion in PBS solution. The laden KaMA supportedin vitrofibroblast growth proliferation. In summary, this research sheds light on KaMA/RBCM hydrogels, could reveal fine-tuned properties manner.

Language: Английский

Citations

9
3D printing of polysaccharide-based formulations: Opportunities for innovation DOI
Fabian Hernández-Tenorio, Edier Múnera-Gutiérrez,

Alejandra M. Miranda

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: unknown, P. e00383 - e00383

Published: Dec. 1, 2024

Language: Английский

Citations

2
Development of foam-based support material for coaxial bioprinting of ionically crosslinking bioinks DOI
Elias Madadian,

Sara Badr,

Debra S. MacDonald

et al.

Bioprinting, Journal Year: 2023, Volume and Issue: 32, P. e00281 - e00281

Published: May 18, 2023

Language: Английский

Citations

5
Nanofibrous Material-Reinforced Printable Ink for Enhanced Cell Proliferation and Tissue Regeneration DOI Creative Commons
Iruthayapandi Selestin Raja, Bongju Kim, Dong‐Wook Han

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(4), P. 363 - 363

Published: April 11, 2024

The three-dimensional (3D) printing of biomaterials, cells, and bioactive components, including growth factors, has gained interest among researchers in the field tissue engineering (TE) with aim developing many scaffolds to sustain size, shape fidelity, structure retain viable cells inside a network. biocompatible hydrogel employed 3D should be soft enough accommodate cell survival. At same time, gel mechanically strong avoid leakage into surrounding medium. Considering these basic criteria, have developed nanocomposite-based printable inks suitable mechanical electroconductive properties. These nanomaterials, carbon family transition metal dichalcogenides, polymeric nanoparticles, act as nanofillers dissipate stress across networks through their electroactive interactions. Nanofiber-reinforced ink is one kind that comprises dispersed nanofiber components matrix. In this current review, we compile various TE applications nanofiber-reinforced describe 3D-printing parameters, classification, impact cross-linkage. Furthermore, discuss challenges future perspectives field.

Language: Английский

Citations

1
A mist-based crosslinking technique for coaxial bioprinting of hollow hydrogel fibers DOI

Sara Badr,

Elias Madadian,

Debra MacDonald

et al.

Bioprinting, Journal Year: 2023, Volume and Issue: 35, P. e00308 - e00308

Published: Aug. 26, 2023

Language: Английский

Citations

2