Aquilaria crassna Extract Exerts Neuroprotective Effect against Benzo[a]pyrene-Induced Toxicity in Human SH-SY5Y Cells: An RNA-Seq-Based Transcriptome Analysis DOI Open Access
Nattaporn Pattarachotanant,

Suporn Sukjamnong,

Panthakarn Rangsinth

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2727 - 2727

Published: Aug. 16, 2024

Benzo[a]pyrene (B[a]P) is known to inhibit neurodifferentiation and induce neurodegeneration. Agarwood or Aquilaria crassna (AC), a plant with health-promoting properties, may counteract the neurotoxic effects of B[a]P by promoting neuronal growth survival. This study investigated protective effect AC leaf ethanolic extract (ACEE) on B[a]P-induced impairment differentiation. A transcriptomic analysis identified canonical pathway, biological network, differentially expressed genes (DEGs) that are changed in response differentiation neurogenesis. Several genes, including CXCR4, ENPP2, GAP43, GFRA2, NELL2, NFASC, NSG2, NGB, BASP1, NEUROD1, B[a]P-treated SH-SY5Y cells were up-regulated after treatment ACEE. Notably, Western blot further confirmed ACEE increased protein levels GAP43 neuroglobin. led decreased phosphorylation Akt ERK cells; however, was able reverse these effects. Clionasterol lupenone Molecular docking showed two phytochemicals had significant interactions GDNF family receptor alpha (GFRA), retinoid X receptors (RXRs). In conclusion, be potential alternative medicine for prevention impaired neurodegenerative diseases.

Language: Английский

A Survey on Computational Methods in Drug Discovery for Neurodegenerative Diseases DOI Creative Commons

Caterina Vicidomini,

Francesco Fontanella, Tiziana D’Alessandro

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(10), P. 1330 - 1330

Published: Oct. 19, 2024

Currently, the age structure of world population is changing due to declining birth rates and increasing life expectancy. As a result, physicians worldwide have treat an number age-related diseases, which neurological disorders represent significant part. In this context, there urgent need discover new therapeutic approaches counteract effects neurodegeneration on human health, computational science can be pivotal importance for more effective neurodrug discovery. The knowledge molecular receptors other biomolecules involved in pathogenesis facilitates design molecules as potential drugs used fight against diseases high social relevance such dementia, Alzheimer's disease (AD) Parkinson's (PD), cite only few. However, absence comprehensive guidelines regarding strengths weaknesses alternative creates fragmented disconnected field, resulting missed opportunities enhance performance achieve successful applications. This review aims summarize some most innovative strategies based methods development. particular, recent applications state-of-the-art docking artificial intelligence ligand- target-based novel drug were reviewed, highlighting crucial role silico context discovery neurodegenerative diseases.

Language: Английский

Citations

4
Effects and mechanisms of water extract of Uraria crinit a on manganese chloride-induced apoptosis in SH-SY5Y cells DOI Creative Commons
Jin–Tung Liang,

Weiwei Yin,

Duanzhuo Li

et al.

CyTA - Journal of Food, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 11, 2025

Language: Английский

Citations

0
Efficacy of Mucuna pruriens (L.) DC. in treating diabetes, Parkinson’s disease, and erectile dysfunction—a review of clinical and preclinical trials DOI Creative Commons
Ravindra Verma, Vaibhav Misra, Prakash S. Bisen

et al.

Published: April 23, 2025

Among the most common medical problems experienced by older adults (over 60 years) are diabetes, Parkinson’s disease (PD), and erectile dysfunction (ED). The potential use of Mucuna pruriens in treating type 2 diabetes (T2D), PD, ED is being investigated. Literature searches were conducted using PubMed, MEDLINE, Mendeley databases (1990–2023). Key words related to pruriens, EDs used. An analysis 26 preclinical clinical trials suggested that may be used treat ED. This study revealed a correlation between Parkinson’s, ED, with metabolic disorders cause. pruriens-based therapeutics could positive source leva-dopa (LD) medications. It well tolerated beneficial for brain function overall health. Evidence suggests it has effects on libido, testosterone levels, PD. important note PD linked multiple mechanisms. In many (in humans animals), found effective at diabetes. further necessary conduct additional scientific studies confirm molecular mechanisms biomarkers link phytochemicals

Language: Английский

Citations

0
Advances in Understanding Biomarkers and Treating Neurological Diseases -Role of the Cerebellar Dysfunction and Emerging Therapies DOI
Azhagu Madhavan Sivalingam

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 102519 - 102519

Published: Sept. 1, 2024

Language: Английский

Citations

3
Exploring Nanocarriers for Boosting Entacapone Bioavailability: A Journey through System Characterization and Assessment of Toxicity and Pharmacological and 2D Permeability Paybacks DOI
Cláudia Sofia Machado, Miguel Pinto, Brandon Aguiar

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(43), P. 58299 - 58312

Published: Oct. 21, 2024

Catechol-O-methyltransferase inhibitors (iCOMT), such as entacapone, have been successfully employed to treat tremor-related symptoms of Parkinson's disease. However, iCOMT has associated with a short half-life and poor oral bioavailability. Nanobased drug delivery systems often used overcome this type setbacks. Therefore, entacapone was encapsulated in PEGylated poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) via nanoprecipitation process, well nanostructured lipid carriers (NLCs) using solvent emulsification/evaporation method. Both nanoformulations presented sub-200 nm populations, zeta-potential (ZP) values close -30 mV, showed stability at different pHs, while maintaining their physicochemical properties mostly intact, presenting only change superficial charge (ZP values), indicating interaction. interaction mucins, which anticipates good permeation bioavailability for topical administration. No cytotoxic effects were observed lyophilized PLGA NPs encapsulating 2-hydroxypropyl-ß-cyclodextrin (HPβCD) cryoprotectant 3% concentration (HP-PLGA@Ent), human hepatocellular carcinoma (HepG2), neuroblastoma (SH-SY5Y), or epithelial colorectal adenocarcinoma (Caco-2) cell lines. Conversely, NLCs (W-NLCs@Ent) on the HepG2 line, likely due intracellular accumulation storage. maintained COMT inhibition effect cells, 3-BTD probe. An increase permeability both monolayer coculture models (Caco-2 Caco-2/HT29-MTX, respectively) developed nanoformulations. Overall, work shows that nanocarriers could be stimulating alternative solve setbacks, since they improve its still inhibitory activity.

Language: Английский

Citations

2
Aquilaria crassna Extract Exerts Neuroprotective Effect against Benzo[a]pyrene-Induced Toxicity in Human SH-SY5Y Cells: An RNA-Seq-Based Transcriptome Analysis DOI Open Access
Nattaporn Pattarachotanant,

Suporn Sukjamnong,

Panthakarn Rangsinth

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2727 - 2727

Published: Aug. 16, 2024

Benzo[a]pyrene (B[a]P) is known to inhibit neurodifferentiation and induce neurodegeneration. Agarwood or Aquilaria crassna (AC), a plant with health-promoting properties, may counteract the neurotoxic effects of B[a]P by promoting neuronal growth survival. This study investigated protective effect AC leaf ethanolic extract (ACEE) on B[a]P-induced impairment differentiation. A transcriptomic analysis identified canonical pathway, biological network, differentially expressed genes (DEGs) that are changed in response differentiation neurogenesis. Several genes, including CXCR4, ENPP2, GAP43, GFRA2, NELL2, NFASC, NSG2, NGB, BASP1, NEUROD1, B[a]P-treated SH-SY5Y cells were up-regulated after treatment ACEE. Notably, Western blot further confirmed ACEE increased protein levels GAP43 neuroglobin. led decreased phosphorylation Akt ERK cells; however, was able reverse these effects. Clionasterol lupenone Molecular docking showed two phytochemicals had significant interactions GDNF family receptor alpha (GFRA), retinoid X receptors (RXRs). In conclusion, be potential alternative medicine for prevention impaired neurodegenerative diseases.

Language: Английский

Citations

1