Investigations of In Vitro Anti-Acetylcholinesterase, Anti-Diabetic, Anti-Inflammatory, and Anti-Cancer Efficacy of Garden Cress (Lepidium sativum Linn.) Seed Extracts, as Well as In Vivo Biochemical and Hematological Assays DOI Creative Commons
Ahmed M. Naglah, Abdulrahman A. Almehizia, Mohamed A. Al‐Omar

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 446 - 446

Published: March 31, 2025

Background/Objectives: The current research was designed to quantify the active phyto-constituents and investigate in vitro biological efficiency of different garden cress (Lepidium sativum Linn.) seed extracts against chronic diseases as well vivo toxicities that may be induced mice upon administration each extract at both studied therapeutic doses. Methods: L. extracts, such methanolic, aqueous, acetone, ethyl acetate assessed. inhibition percentage (%) median inhibitory concentration (IC50) values were estimated acetylcholinesterase enzyme, diabetes mellitus (α-amylase α-glucosidase enzymes), inflammation (cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX) enzymes). Additionally, HepG-2, Caco-2, A549 cells assessed using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Moreover, might hematological (using an automatic blood analyzer) biochemical levels evaluated. Results: It found methanolic possessed highest activities compared other extracts. 51.34, 54.35, 44.10, 43.48, 40.78% acetylcholinesterase, α-amylase, α-glucosidase, protein denaturation, proteinase enzymes, respectively. also exhibited effect COX-1 (55.05%), COX-2 (57.30%), 5-LOX (50.15%) enzymes. possesses cytotoxic activity cells, with IC50 52.27, 40.73, 37.95 μg/mL, lethal doses (LD50) showed safer when administered orally, followed by aqueous then extract. acetone no alterations orally two (1/10 1/20 LD50) control. Conclusions: This study concluded than In future, efficacy will evaluated, elucidation its mechanism diseases.

Language: Английский

Investigations of In Vitro Anti-Acetylcholinesterase, Anti-Diabetic, Anti-Inflammatory, and Anti-Cancer Efficacy of Garden Cress (Lepidium sativum Linn.) Seed Extracts, as Well as In Vivo Biochemical and Hematological Assays DOI Creative Commons
Ahmed M. Naglah, Abdulrahman A. Almehizia, Mohamed A. Al‐Omar

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 446 - 446

Published: March 31, 2025

Background/Objectives: The current research was designed to quantify the active phyto-constituents and investigate in vitro biological efficiency of different garden cress (Lepidium sativum Linn.) seed extracts against chronic diseases as well vivo toxicities that may be induced mice upon administration each extract at both studied therapeutic doses. Methods: L. extracts, such methanolic, aqueous, acetone, ethyl acetate assessed. inhibition percentage (%) median inhibitory concentration (IC50) values were estimated acetylcholinesterase enzyme, diabetes mellitus (α-amylase α-glucosidase enzymes), inflammation (cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX) enzymes). Additionally, HepG-2, Caco-2, A549 cells assessed using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Moreover, might hematological (using an automatic blood analyzer) biochemical levels evaluated. Results: It found methanolic possessed highest activities compared other extracts. 51.34, 54.35, 44.10, 43.48, 40.78% acetylcholinesterase, α-amylase, α-glucosidase, protein denaturation, proteinase enzymes, respectively. also exhibited effect COX-1 (55.05%), COX-2 (57.30%), 5-LOX (50.15%) enzymes. possesses cytotoxic activity cells, with IC50 52.27, 40.73, 37.95 μg/mL, lethal doses (LD50) showed safer when administered orally, followed by aqueous then extract. acetone no alterations orally two (1/10 1/20 LD50) control. Conclusions: This study concluded than In future, efficacy will evaluated, elucidation its mechanism diseases.

Language: Английский

Citations

0