Assessment of Extracellular Particles Directly in Diluted Plasma and Blood by Interferometric Light Microscopy. A Study of 613 Human and 163 Canine Samples DOI Creative Commons

Boštjan Korenjak,

Armando Tratenšek,

Matevž Arko

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2054 - 2054

Published: Dec. 12, 2024

Extracellular nanoparticles (EPs) are a subject of increasing interest for their biological role as mediators in cell–cell communication; however, harvesting and assessment from bodily fluids challenging, processing can significantly affect samples. With the aim minimizing artifacts, we assessed number density (n) hydrodynamic diameter (Dh) EPs directly diluted plasma blood using following recently developed technique: interferometric light microscopy (ILM). We analyzed 613 samples human patients with inflammatory bowel disease (IBD), collected trisodium citrate ethylenediaminetetraacetic acid (EDTA) anticoagulants, 163 canine brachycephalic obstructive airway syndrome (BOAS). found highly statistically significant correlation between n only (i.e., but not canine) samples, EDTA, respective Dh both species (all p < 10−3). In plasma, average <Dh> was 139 ± 31 nm; blood, 158 11 155 32 171 33 nm. The differences within were (p 10−2), sufficient statistical power (P > 0.8). For <n>, no or EDTA. Our results prove that measuring minimally processed fresh by means ILM is feasible large populations

Language: Английский

Nanoparticle Tracking Analysis: An Effective Tool to Characterize Extracellular Vesicles DOI Creative Commons
Gabrielle Kowkabany, Yuping Bao

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4672 - 4672

Published: Oct. 1, 2024

Extracellular vesicles (EVs) are membrane-enclosed particles that have attracted much attention for their potential in disease diagnosis and therapy. However, the clinical translation is limited by dosing consistency due to heterogeneity. Among various characterization techniques, nanoparticle tracking analysis (NTA) offers distinct benefits EV characterization. In this review, we will discuss NTA technique with a focus on factors affecting results; then, review two modes of techniques along suitable applications specific areas studies. EVs typically characterized size, size distribution, concentration, protein markers, RNA cargos. The light-scattering mode accurate concentration information solution, which useful comparing isolation methods, storage conditions, secretion conditions. contrast, fluorescent allows differentiating subgroups based markers. success fluorescence heavily relies tags (e.g., types dyes labeling methods). When labeled disease-specific an effective tool detection biological fluids, such as saliva, blood, serum. Finally, limitations future directions

Language: Английский

Citations

14
Unignored intracellular journey and biomedical applications of extracellular vesicles DOI

Jiuhong Zhao,

Wenjing Zhu,

Yuxuan Mao

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 212, P. 115388 - 115388

Published: July 3, 2024

Language: Английский

Citations

5
The Role of Exosomes in Cancer Progression and Therapy DOI Creative Commons
Shynggys Sergazy,

Roza Seydahmetova,

Alexander Gulyayev

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 27 - 27

Published: Jan. 1, 2025

Exosomes are small extracellular vesicles and crucial in intercellular communication. Interestingly, tumor-derived exosomes carry oncogenic molecules, such as proteins microRNAs, which can reprogram recipient cells, promote angiogenesis, stimulate cancer pre-metastatic niche, supporting growth metastasis. On the other hand, their biocompatibility, stability, ability to cross biological barriers make them attractive candidates for drug delivery. Recent advances have shown potential be used early disease detection targeted therapy by delivering therapeutic agents specifically tumor sites. Despite promising applications, a number of challenges remain, including exosome isolation characterization, well inherent heterogeneity. Thus, current review aims describe roles health disease, discuss that hinder development into becoming useful medical tools.

Language: Английский

Citations

0
Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Boost Functional Performance in an Animal Model of Multiple Sclerosis Through Recruiting Oligodendrocytes and Attenuating Gliosis DOI

S Mohammadhadi Mirab,

Ameneh Omidi,

Masoud Soleimani

et al.

Stem Cell Reviews and Reports, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

Citations

0
Methods for Extracellular Vesicle Isolation: Relevance for Encapsulated miRNAs in Disease Diagnosis and Treatment DOI Open Access

María Ljungström,

Elisa Oltra

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 330 - 330

Published: March 12, 2025

Extracellular vesicles (EVs) are nanovesicles that facilitate intercellular communication by carrying essential biomolecules under physiological and pathological conditions including microRNAs (miRNAs). They found in various body fluids, such as blood, urine, saliva, their levels fluctuate with disease progression, making them valuable diagnostic tools. However, isolating EVs is challenging due to small size biological complexity. Here, we summarize the principles behind most common EV isolation methods ultracentrifugation, precipitation, immunoaffinity, sorting, ultrafiltration, exclusion chromatography, microfluidics while highlighting protocol strengths weaknesses. We also review main strategies identify quantify circulating miRNAs a particular focus on EV-encapsulated miRNAs. Since these hold special clinical interest derived from superior stability therapeutic potential, information provided here should provide guidance for future research initiatives promising field of treatment based

Language: Английский

Citations

0
Exosomal biomarkers: A novel frontier in the diagnosis of gastrointestinal cancers DOI
Yuan Zhang,

Ning-Ning Yue,

Liyu Chen

et al.

World Journal of Gastrointestinal Oncology, Journal Year: 2025, Volume and Issue: 17(4)

Published: March 24, 2025

Gastrointestinal (GI) cancers, which predominantly manifest in the stomach, colorectum, liver, esophagus, and pancreas, accounting for approximately 35% of global cancer-related mortality. The advent liquid biopsy has introduced a pivotal diagnostic modality early identification premalignant GI lesions incipient cancers. This non-invasive technique not only facilitates prompt therapeutic intervention, but also serves as critical adjunct prognosticating likelihood tumor recurrence. wealth circulating exosomes present body fluids is often enriched with proteins, lipids, microRNAs, other RNAs derived from cells. These specific cargo components are reflective processes involved tumorigenesis, progression, response to treatment. As such, they represent group promising biomarkers aiding diagnosis cancer. In this review, we delivered an exhaustive overview composition pathways sorting within these vesicles. We laid out some clinical evidence that supported utilization cancers discussed their potential application. Furthermore, addressed challenges encountered when harnessing predictive instruments realm

Language: Английский

Citations

0
Cutting‐Edge Aptasensing Approaches for Electrochemical Detection of Exosomes Utilizing MXenes DOI Creative Commons

Andrej Vikartovský,

Natália Košútová, Lenka Lorencová

et al.

ChemElectroChem, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Herein, the basic properties, composition, and isolation methods for separation of exosomes are described, since they can be a rich source disease biomarkers. Then an introduction to MXenes, novel class 2D nanomaterials with interesting properties applicable in numerous fields including biosensing is provided. Also, aptamers described as alternative antibodies robust biorecognition analytes interest. The final part article gives examples which these three key components integrated sensitive and/or electrochemical detection exosomes. conclusion provides summary initial achievements also outlook future discoveries exosome aptasensing using advanced nanomaterials, i.e., MXenes. MXenes have promising affinity‐based biosensing, being hydrophilic surface functional groups. In addition, free plasmons present used covalent grafting elements diazonium moieties. This especially approach immobilization DNA/RNA aptamers, readily modified by

Language: Английский

Citations

0
From Bench to Bedside: The Evolution of Extracellular Vesicle Diagnostics through Microfluidic and Paper-Based Technologies DOI
P. Gopalan,

Arjun Jayachandran,

Sasidhar Manda Venkata

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: unknown, P. 114675 - 114675

Published: April 1, 2025

Language: Английский

Citations

0
Mesenchymal stem cell exosomes therapy for the treatment of traumatic brain injury: mechanism, progress, challenges and prospects DOI Creative Commons
Ming-wei Liu, Hua Li,

Gang Xiong

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 11, 2025

Language: Английский

Citations

0
Plant-Derived Exosome-Like Nanoparticles Based Treatments In Cancer Therapy DOI
Gaye Umurhan, Meriç Arda Eşmekaya, Burhan Ertekin

et al.

Journal of Gazi University Health Sciences Institute, Journal Year: 2025, Volume and Issue: 7(1), P. 19 - 27

Published: April 30, 2025

Plant-derived exosome-like vesicles (PELVs) are nanometer-sized particles comprising proteins, lipids, nucleic acids, and small molecule substances generated from plants. PELVs have many advantages, such as low toxicity, efficient cellular uptake, high biocompatibility, stability, large-scale production. can regulate intercellular communication by releasing their contents, including mRNA, miRNA, proteins. (PDELVs) attracted considerable attention in scientific research owing to promising therapeutic effects researches assessed the extensive potential of PDELVs treatment various diseases cancer treatment. They exhibit clinical attributes benefits over conventional pharmaceuticals. This mini-review aims summarize categorize main paths followed scientists working with PDELNs for therapy.

Language: Английский

Citations

0