Development of robust in vitro propagation protocol and cyto-genetic fidelity assessment of Siraitia grosvenorii (monk fruit)
Scientia Horticulturae,
Journal Year:
2024,
Volume and Issue:
331, P. 113142 - 113142
Published: March 29, 2024
Language: Английский
Callus-Mediated High-Frequency Plant Regeneration, Phytochemical Profiling, Antioxidant Activity and Genetic Stability in Ruta chalepensis L.
Plants,
Journal Year:
2022,
Volume and Issue:
11(12), P. 1614 - 1614
Published: June 20, 2022
Efficient
methods
for
callus
induction
and
the
high-frequency
plant
regeneration
of
Ruta
chalepensis
L.
were
established,
phytochemical
potential
antioxidant
activity
a
donor
plant,
ex-vitro-established
micropropagated
plants,
also
studied.
Yellowish-green
was
induced
with
frequency
97.8%
from
internode
shoot
segments
growing
in
soil
botanical
garden
cultured
on
Murashige
Skoog
(MS)
medium
containing
10
μM
2,4-D
(2,4-dichlorophenoxyacetic
acid)
1
BA
(6-benzyladenine).
Adventitious
shoots
regenerated
yellowish-green
MS
5.0
(BA)
1.0
1-naphthaleneacetic
acid
(NAA),
98.4%
maximum
54.6
an
average
length
4.5
cm
after
8
weeks.
The
rooted
IBA
(indole-3-butyric
successfully
transferred
to
ex
vitro
conditions
pots
normal
soil,
95%
survival
rate.
amounts
alkaloids,
phenolics,
flavonoids,
tannins,
plants
higher
than
callus.
highest
contents
hesperidin
rutin
(93.3
55.9
µg/mg,
respectively)
found
compared
those
obtained
(91.4
31.0
(59.1
21.6
respectively).
genetic
uniformity
appraised
by
ISSR
markers
plant.
This
is
first
report
describing
callus-mediated
regeneration,
as
well
production
phenolic
compounds
activities
R.
chalepensis,
which
might
be
alternative
technique
mass
propagation
synthesis
bioactive
such
rutin.
Language: Английский
Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of Maerua crassifolia Forssk
Forests,
Journal Year:
2023,
Volume and Issue:
14(6), P. 1160 - 1160
Published: June 5, 2023
The
difficult
propagation
of
shrub
and
tree
species
their
extensive
exposure
to
grazing
threaten
abundance
lead
the
necessity
find
alternative
means
for
these
species.
In
vitro
micropropagation
techniques,
viz.,
tissue
culture,
offer
a
promising
tool
rapid,
cost-effective,
efficient
different
plant
current
study,
rapid
in
multiplication
protocol
was
developed
Maerua
crassifolia
Forssk.
Our
results
revealed
that
Murashige
Skoog
(MS)
medium
with
7.5
µM
6-benzylaminopurine
(BA)
1.0
1-naphthaleneacetic
acid
(NAA)
led
highest
shoot
formation
(13.9
shoots
per
explant
85.7%
cultivated
hypocotyls)
among
all
other
treatments.
best
root
obtained
on
half-strength
MS
indole
3-butyric
(IBA)
as
94.1%
formed
6.8
roots
microshoot
average.
Ninety
percent
rooted
plantlets
were
successfully
acclimatized
are
currently
growing
botanical
garden
Botany
Microbiology
Department,
King
Saud
University,
Riyadh,
Saudi
Arabia.
genetic
fidelity
micropropagated
plants
authenticated
via
flow
cytometry.
study
explained
simple,
endangered
M.
trees.
Language: Английский
In vitro flower induction and cyto-genetic fidelity assessment of Chlorophytum comosum (Thunb.) Jacques var. comosum
Jhilmil Nath,
No information about this author
Kiran Devi,
No information about this author
Vikas Kumar
No information about this author
et al.
South African Journal of Botany,
Journal Year:
2023,
Volume and Issue:
159, P. 678 - 685
Published: July 12, 2023
Language: Английский
Establishment of an Efficient In Vitro Propagation Method for a Sustainable Supply of Plectranthus amboinicus (Lour.) and Genetic Homogeneity Using Flow Cytometry and SPAR Markers
Horticulturae,
Journal Year:
2022,
Volume and Issue:
8(8), P. 693 - 693
Published: Aug. 1, 2022
Plectranthus
amboinicus
(Lour.)
Spreng
is
a
medicinally
important
aromatic
perennial
herb
used
for
the
treatment
of
skin
diseases,
constipation,
asthma,
flu,
fever,
cough,
and
headache
as
well
flavoring
ingredient
in
traditional
drinks,
food,
meat
stuffing.
In
this
study,
high-performance
vitro
propagation
system
P.
through
direct
shoot
organogenesis
was
developed
using
axillary
node
explants
cultured
on
MS
(Murashige
Skoog)
medium
augmented
with
0.5,
2.5,
5.0,
7.5,
10.0
µM
6-benzyladenine
(BA)
or
kinetin
(Kin),
alone
0.1,
5.0
indole-3-acetic
acid
(IAA)
α-naphthalene
acetic
(NAA).
To
optimize
regeneration
potential
explants,
effects
basal
media
strength
pH
were
also
investigated.
After
8
weeks
culture,
full
(pH
5.7)
BA
2.5
NAA
exhibited
highest
percentage
(97.1%)
maximum
number
(19.3)
shoots
per
explant.
Individual
elongated
rooted
half
containing
0.25
indole
3-butyric
(IBA)
after
4
producing
5.3
roots/shootlets
root
induction
frequency
93.7%.
First
time
genetic
stability
raised
plants
determined
SPAR
markers,
such
DAMD
ISSR,
flow
cytometric
tests,
assuring
availability
authenticated
raw
materials
commercial
production
plant
its
bioactive
components.
Language: Английский
Thidiazuron Induced In Vitro Clonal Propagation of Lagerstroemia speciosa (L.) Pers.—An Important Avenue Tree
Horticulturae,
Journal Year:
2022,
Volume and Issue:
8(5), P. 359 - 359
Published: April 20, 2022
A
high
throughput
regeneration
protocol
has
been
developed
for
Lagerstroemia
speciosa
through
node
explants
under
the
regime
of
various
plant
growth
regulators
(PGRs).
This
can
provide
an
alternative
mode
to
seed-grown
plants
and
minimize
cost–time
regeneration,
significantly.
Murashige
Skoog
(MS)
medium
containing
combinations
PGRs
exhibited
a
marked
stimulatory
effect
on
morphogenesis.
Of
tried,
explant
pretreated
with
thidiazuron
(TDZ;
5.0
µM)
4
weeks
followed
transfer
into
MS
1.0
μM
6-benzyladenine
(BA)
0.25
α-naphthalene
acetic
acid
(NAA)
was
reported
be
best
treatment
as
it
resulted
in
maximum
number
24.5
shoots
average
shoot
length
7.1
cm
per
90%
cultures
after
12
incubation.
The
vitro-generated
rooted
satisfactorily
adopted
ex
vitro
method
rooting,
which
saves
time
cost.
Among
different
treatments,
greatest
rooting
percentage
(85%)
observed
200
IBA-treated
shoots,
highest
root
(8.7)
(3.4
cm)
occurring
weeks.
Four
months
being
transferred
vitro,
some
physiological
attributes
vitro-propagated
were
examined
compared
plants.
Further,
analysis
genetic
integrity
tissue
culture-raised
plantlets
along
parental
tree
accomplished
DNA-based
RAPD
technique.
monomorphic
banding
pattern
obtained
by
primers
level
uniformity
regenerated
Language: Английский
In vitro regeneration, phytochemical profiling and antioxidant activity in Ruta chalepensis plants established from alginate encapsulated synthetic seeds
South African Journal of Botany,
Journal Year:
2023,
Volume and Issue:
161, P. 575 - 585
Published: Sept. 1, 2023
Language: Английский
Molecular Markers for the Detection and Analysis of Somaclonal Variation
Springer eBooks,
Journal Year:
2024,
Volume and Issue:
unknown, P. 57 - 82
Published: Jan. 1, 2024
Language: Английский
Micropropagation and Genetic Fidelity of Fegra Fig (Ficus palmata Forssk.) and Grafting Compatibility of the Regenerated Plants with Ficus carica
Plants,
Journal Year:
2024,
Volume and Issue:
13(9), P. 1278 - 1278
Published: May 6, 2024
is
an
important
fig
species
that
produces
edible
and
nutritious
fruit
possesses
several
therapeutic
uses.
This
study
reports
effective
method
for
the
micropropagation
of
Language: Английский
Molecular Techniques for the Detection of Ploidy Level and Genetic Fidelity of Regenerated Plantlets
BENTHAM SCIENCE PUBLISHERS eBooks,
Journal Year:
2023,
Volume and Issue:
unknown, P. 294 - 326
Published: Sept. 11, 2023
Most
of
the
medicinal,
aromatic
and
other
commercially
important
crops
are
poor
rooters,
some
them
sessile
in
terms
seed
production;
hence
these
plants
very
difficult
to
propagate
either
through
stem
cuttings
based
vegetative
propagation
or
seedlings
sexual
propagation.
During
last
two
decades,
plant
tissue
culture
has
emerged
as
an
alternative
technique
for
with
commercial
importance.
Majorly,
somatic
tissues,
viz.,
leaf,
node
shoot
tip,
being
used
explants
production
genetically
similar
plantlets
studies.
Recently,
abnormalities
respect
ploidy
level
genetic
fidelity
have
been
reported
In
Vitro
regenerated
plantlets.
This
is
mainly
due
usage
synthetic
chemicals
artificial
growth
regulators
studies,
fragile
nature
callus
exposure
cultures
light
sources.
order
ensure
true
clones
plants,
nowadays
it
become
obligatory
step
assess
that
mother
plants.
book
chapter
focussed
on
different
molecular
techniques
which
use
detection
micro
propagated
Language: Английский