Engineering Drought Tolerance in Crops Using CRISPR Cas systems DOI Open Access
Халмирзаева Лола, Mukhammadjon K. Mirzakhmedov, Mirzakamol S. Ayubov

et al.

Plant Science Today, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 29, 2023

Drought stress is one of the most considerable threats to global agricultural food security, causing yield losses worldwide. Therefore, search for effective genetic and molecular methods developing cultivars that are tolerant or resistant harsh environments has been more intensive over last decades. Apart from time-consuming conventional breeding techniques, biotechnologists now investigating modern genome editing tools engineering tolerance resistance various biotic abiotic stresses in crops. Various techniques such as zinc finger nucleases (ZFNs) transcription activator-like effector (TALENs) were developed based on discovery DNA structure. However, these have limitations, with ZFNs being prone errors due their limited base pair recognition, TALENs requiring a complex protein process struggling cleave methylated DNA. In recent years, clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) its alternatives gained popularity plant biotechnology. Out mentioned earlier, CRISPR/Cas9 becoming popular because it's faster easier use. Given drought significant threat agriculture drying arable lands, this review focuses how we can use CRISPR enhance crop explores future potential.

Language: Английский

CRISPR/Cas9-mediated knockout strategies for enhancing immunotherapy in breast cancer DOI
Chenchen Xu

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: 397(11), P. 8561 - 8601

Published: June 22, 2024

Language: Английский

Citations

1
Mechanism analysis of low nighttime temperature increasing erucic acid content in Brassica napus L. Seeds DOI
Chao Mi, Yanning Zhao,

Liangbin Lin

et al.

Gene, Journal Year: 2024, Volume and Issue: unknown, P. 149119 - 149119

Published: Nov. 1, 2024

Language: Английский

Citations

1
Plant Functional Genomics Based on High‐Throughput CRISPR Library Knockout Screening: A Perspective DOI Creative Commons

Jianjie He,

Can Zeng, Maoteng Li

et al.

Advanced Genetics, Journal Year: 2023, Volume and Issue: 5(1)

Published: Nov. 27, 2023

Abstract Plant biology studies in the post‐genome era have been focused on annotating genome sequences’ functions. The established plant mutant collections greatly accelerated functional genomics research past few decades. However, most sequences' roles and underlying regulatory networks remain substantially unknown. Clustered, regularly interspaced short palindromic repeat (CRISPR)‐associated systems are robust, versatile tools for manipulating genomes with various targeted DNA perturbations, providing an excellent opportunity high‐throughput interrogation of elements’ roles. This study compares methods frequently used then discusses different multi‐targeted strategies to overcome gene redundancy using CRISPR‐Cas9 system. Next, this work summarizes recent reports CRISPR libraries knockout function discoveries plants. Finally, envisions future perspective optimizing leveraging library screening genomes' other uncharacterized sequences.

Language: Английский

Citations

2
Mutagenesis Application in Plant Improvement: Advancements and Its Future DOI
Augustine Antwi-Boasiako,

Padmore Adu-Antwi,

R. Adu Amoah

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: July 12, 2024

Agricultural plant genetic resources are constantly going into extinction having negative implications for banks. Hence, there is a need to generate variations. Stimulated mutagenesis offers an efficient tool variation and explore the function of genes. It also facilitates identification genes their roles in traits economic interest breeders, farmers consumers. Thus, transforming agro-based industries overcoming obstacles (poor yield, lodging, shattering, pests disease infestations). Exploring alternatives integrate farmers’ consumers’ desirable preferred cultivars has led major advancements mutation breeding. The chapter provides comprehensive update on induced approaches, increasing efficiency targeted novel mutated populations. Furthermore, it reveals efforts ten countries that leading development varieties via across globe most prioritised crops have received critical attention Moreover, seeks bring light current approaches used facilitating details progress made improving plants with evidence relating generating resources, biotic abiotic stresses, nutritional, quality improvement whiles providing future directions

Language: Английский

Citations

0
Genome-wide evaluation of gene editing outcomes using CRISPR/Cas9 in seed propagated Camelina sativa and vegetatively propagated Solanum tuberosum DOI Creative Commons
Thilani B. Jayakody,

Daniel G. Zarka,

Keun H. Cho

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 26, 2024

CRISPR/Cas9 is the most popular genome editing platform for investigating gene function or improving traits in plants. The specificity of has yet to be evaluated at a genome-wide scale seed-propagated

Language: Английский

Citations

0
Transitioning from the Green Revolution to the Gene Revolution: strengthening nutritional security using climate resilient traditional crops DOI Creative Commons
Rushikesh Tahakik,

Amit G Deshmukh,

M. P. Moharil

et al.

Bulletin of the National Research Centre/Bulletin of the National Research Center, Journal Year: 2024, Volume and Issue: 48(1)

Published: Dec. 9, 2024

Abstract Background World food production has significantly increased, largely attributed to advancements in breeding methods pioneered previous decades. These can be categorized into three major eras—the pre-Green Revolution era, the Green and post-Green era—which we call Gene Revolution. The era was characterized by utilization of mutation forward genetics approaches. Main body (1950s–1980s) brought about improvements crop breeding, irrigation, fertilizer use, leading a substantial rise reduced rural poverty. However, focus primarily on yield, with less nutritional quality. After Revolution, wheat rice rapidly increased from 0.4–2 metric tonnes per hectare 2–4 due government initiatives. Nevertheless, cultivation other crops, including indigenous varieties millet, decreased. In while advanced genome editing technologies such as CRISPR‒Cas9 TALENs have enhanced content climate resilience there also been renewed reviving crops. Conclusion This review discusses bring these orphan nutria crops back emphasizes importance passing this knowledge future generations, ensuring their place sustainable agriculture security.

Language: Английский

Citations

0
Edición de Genes y CRISPR-Cas: Aplicaciones, Avances y Desafíos: Genes y CRISPR DOI
Joel Horacio Elizondo-Luévano, Lizeth Aniram Garcia Sotelo, Ivan Cárdenas Paredes

et al.

Published: May 31, 2024

La tecnología de CRISPR-Cas es una herramienta altamente efectiva para la edición genes, con el objetivo evaluar y describir fundamento, analizar los avances desafíos en genes un enfoque específico campo agricultura, se realizó revisión literatura bases datos como PubMed, Google académico, Scielo, Science Direct, entre años 2011 2024. Utilizando palabras clave `Ingeniería genética’, `Agricultura’, `CRISPR-Cas’. El método CRISPR-Cas9 usa dos tipos RNA endonucleasa Cas. proteína Cas sirve cortar ADN virus extraño fragmentos añade a secuencia CRISPR espaciadores. Por consiguiente, transcribe generar crARN tracrARN que posteriormente unen formar complejo gARN. sistema introduce célula mediante plásmidos, ARN o ribonucleoproteínas. Una posibilita al núcleo. útil lograr gen especifico sean gran interés gremio científico.

Citations

0
The Search of a Molecular “Swiss Knife” for Chloroplast Genomic Editing DOI Creative Commons
Н. В. Дорогова, Yuriy V. Sidorchuk

Horticulturae, Journal Year: 2023, Volume and Issue: 9(12), P. 1338 - 1338

Published: Dec. 14, 2023

In recent years, genome editing methods have become an integral part of the genetic engineering toolset that allows for making targeted changes to plant genomes, both in case single-gene mutations and multiplex modifications. These technologies were mostly proven effective nuclear genomes. However, plastids, best-known example which is chloroplasts, their own (plastome), also available various manipulations, including editing. Despite fact modification plastomes represents a very promising task modern biotechnology, structure plastids peculiarities organization require specific adaptation methods. This applies design constructs delivery plastids. The article provides overview current state research field plastid with chloroplasts taken as example. We consider possibilities using programmable genome-editing technologies, analyze effectiveness, limitations, problems caused by structural features these organelles, organization. discuss results first successful experiments this try assess prospects development tools increasing efficiency specificity biotechnological platform.

Language: Английский

Citations

1
Engineering Drought Tolerance in Crops Using CRISPR Cas systems DOI Open Access
Халмирзаева Лола, Mukhammadjon K. Mirzakhmedov, Mirzakamol S. Ayubov

et al.

Plant Science Today, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 29, 2023

Drought stress is one of the most considerable threats to global agricultural food security, causing yield losses worldwide. Therefore, search for effective genetic and molecular methods developing cultivars that are tolerant or resistant harsh environments has been more intensive over last decades. Apart from time-consuming conventional breeding techniques, biotechnologists now investigating modern genome editing tools engineering tolerance resistance various biotic abiotic stresses in crops. Various techniques such as zinc finger nucleases (ZFNs) transcription activator-like effector (TALENs) were developed based on discovery DNA structure. However, these have limitations, with ZFNs being prone errors due their limited base pair recognition, TALENs requiring a complex protein process struggling cleave methylated DNA. In recent years, clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) its alternatives gained popularity plant biotechnology. Out mentioned earlier, CRISPR/Cas9 becoming popular because it's faster easier use. Given drought significant threat agriculture drying arable lands, this review focuses how we can use CRISPR enhance crop explores future potential.

Language: Английский

Citations

0