Integrated Management of Pathogens and Microbes on <em>Cannabis sativa</em> L. (Cannabis) under Greenhouse Conditions

Published: Feb. 6, 2024

The increased cultivation of high THC-containing Cannabis sativa L. (cannabis), particularly in greenhouses, has resulted a greater incidence diseases and molds that can negatively affect the growth quality crop. Among them, most important are root rots (Fusarium Pythium spp.), Botrytis bud rot (Botrytis cinerea), powdery mildew (Golovinomyces ambrosiae), cannabis stunt disease (caused by Hop latent viroid), range microbes reduce post-harvest quality. An integrated management approach to impact these diseases/microbes requires combining different approaches target reproduction, spread survival associated pathogens, many which occur on same plant simultaneously. These will be discussed context developing an plan manage pathogens greenhouse-grown at stages development. include maintenance stock plants, propagation through cuttings, vegetative flowering. genotypes with tolerance or resistance various is very approach, followed pathogen-free plants. When combined cultural (sanitation, irrigation, monitoring for diseases) environmental (greenhouse climate modification), significant reduction pathogen development achieved. use preventive applications microbial biological control agents reduced risk biorational products also all production jurisdictions where they registered use. promising strategies plants during greenhouse reviewed. Future areas research identified.

Language: Английский

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Integrated Management of Pathogens and Microbes in Cannabis sativa L. (Cannabis) under Greenhouse Conditions

Plants, Journal Year: 2024, Volume and Issue: 13(6), P. 786 - 786

Published: March 10, 2024

The increased cultivation of high THC-containing Cannabis sativa L. (cannabis), particularly in greenhouses, has resulted a greater incidence diseases and molds that can negatively affect the growth quality crop. Among them, most important are root rots (Fusarium Pythium spp.), bud rot (Botrytis cinerea), powdery mildew (Golovinomyces ambrosiae), cannabis stunt disease (caused by hop latent viroid), range microbes reduce post-harvest quality. An integrated management approach to impact these diseases/microbes requires combining different approaches target reproduction, spread, survival associated pathogens, many which occur on same plant simultaneously. These will be discussed context developing an plan manage pathogens greenhouse-grown at stages development. include maintenance stock plants, propagation through cuttings, vegetative flowering. genotypes with tolerance or resistance various is very approach, as well pathogen-free plants. When combined cultural (sanitation, irrigation, monitoring for diseases) environmental (greenhouse climate modification), significant reduction pathogen development spread achieved. use preventive applications microbial biological control agents reduced-risk biorational products also all production jurisdictions where they registered use. promising strategies plants during greenhouse reviewed. Future areas research identified.

Language: Английский

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Reduction of flavonoid content in honeysuckle via Erysiphe lonicerae-mediated inhibition of three essential genes in flavonoid biosynthesis pathways

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: April 16, 2024

Honeysuckle, valued for its wide-ranging uses in medicine, cuisine, and aesthetics, faces a significant challenge cultivation due to powdery mildew, primarily caused by the Erysiphe lonicerae pathogen. The interaction between honeysuckle E. , especially concerning disease progression, remains insufficiently understood. Our study, conducted three different locations, found that naturally infected with showed notable decreases total flavonoid content, reductions of 34.7%, 53.5%, 53.8% observed each respective site. Controlled experiments supported these findings, indicating artificial inoculation led 20.9% reduction levels over 21 days, worsening 54.8% decrease day 42. Additionally, there was drop plant’s antioxidant capacity, reaching an 81.7% 56 days after inoculation. Metabolomic analysis also revealed substantial essential medicinal components such as chlorogenic acid, luteolin, quercetin, isoquercetin, rutin. Investigating gene expression marked relative LjPAL1 gene, starting early 7 post-inoculation falling minimal level (fold change = 0.29) 35. This trend mirrored consistent phenylalanine ammonia-lyase activity through entire process, which decreased 72.3% 56. Further sustained repression downstream genes LjFNHO1 LjFNGT1 closely linked . We identified mechanism inhibits this pathway suggest may strategically weaken honeysuckle’s resistance targeting key biosynthetic pathways, thereby facilitating further pathogen invasion. Based on our we recommend two primary strategies: first, monitoring constituent from -affected areas ensure therapeutic effectiveness; second, emphasizing prevention control measures against mildew persistent decline crucial active compounds.

Language: Английский

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Transcriptional time-course analysis during ash dieback infection revealed different responses in tolerant and susceptible Fraxinus excelsior genotypes

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 25, 2025

Abstract Hymenoscyphus fraxineus , the causal agent of Ash Dieback (ADB), has been introduced to eastern Europe in 1990s from where it spread causing decline European ash populations. However, genetic basis molecular response tolerant and susceptible trees this disease is still largely unknown. We performed RNA-sequencing study transcriptomic four genotypes (ADB-tolerant FAR3 FS36, ADB-susceptible UW1 UW2), during a time-course 7, 14, 21, 28 days post-inoculation, including mock-inoculated as control samples for each sampling time point. The analysis yielded 395 500 Differentially Expressed Genes (DEGs) along ADB-tolerant respectively, while UW2 revealed 194 571 DEGs, with most DEGs found exclusively just one genotypes. shared between included genes involved production phytoalexins other secondary metabolites roles plant defense. Moreover, we identified an earlier expression both pattern- effector-triggered immunity (PTI ETI) genotypes, responses were delayed (late response). Overall, these results different patterns not only but also within two groups. This hints individual natural tolerance ADB, possibly revealing diversified strategies across

Language: Английский

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Mapping and characterization of a novel powdery mildew resistance locus (PM2) in Cannabis sativa L.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 13, 2025

Introduction Breeding genetic resistance to economically important crop diseases is the most sustainable strategy for disease management and enhancing agricultural horticultural productivity, particularly where application of synthetic pesticides prohibited. Powdery mildew disease, caused by biotrophic fungal pathogen Golovinomyces ambrosiae , one prevalent threats cannabis hemp industry worldwide. Methods In this study, we used bulked-segregant analysis combined with high-throughput RNA sequencing (BSRSeq) identify map a novel single dominant (R) locus (designated PM2), that strongly suppresses powdery infection sporulation in Cannabis sativa . Results discussion BSA mapped PM2 chromosome 9. Histochemical revealed PM2-induced mediated highly localized hypersensitive response mainly epidermal cells host. Importantly, markers capable tracking breeding populations were developed using associated SNPs identified study. The ability track will allow successful introgression PM into elite cultivars help move towards more industry.

Language: Английский

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Identification and Transfer of a New Powdery Mildew Resistance Gene PmCAHM from Landrace Changanhongmai into Common Wheat

Agronomy, Journal Year: 2024, Volume and Issue: 14(4), P. 667 - 667

Published: March 25, 2024

Powdery mildew is a severe wheat disease that causes substantial yield losses in production worldwide. The Chinese landrace Changanhongmai (CAHM) exhibits high resistance to the physiological race E09 of powdery mildew. In this study, we characterized gene CAHM, and developed molecular markers for marker-assisted selection. To investigate genetic characteristics resistant gene, F1 plants, F2 generation population, F2:3 families by crossing CAHM with SY225 (Shaanyou ‘225’ as susceptible male parent). Genetic analysis demonstrated all plants were disease, while ratio was 3: 1 both population families, indicating inherited manner single dominant which tentatively designated PmCAHM. By using bulk segregation analysis, constructed map encompassing Xgwm273, Xwmc626, Xgwm11, Xgwm18, Xgdm28, Xgpw7812, Xgpw5195, Xwmc694, Among these markers, Xgpw7812 Xgpw5195 are flanking tightly linked PmCAHM at distance 2.5 cM 8.4 cM, respectively. Furthermore, nullisomic-tetrasomic revealed located on chromosome 1B. These results indicate differs from internationally recognized genes location source. addition, new germplasm/line NW1748 stronger large grains cross backcross populations Fengyou1718 (FY1718)/CAHM/5/FY 1718. Therefore, can serve novel source breeding donor future.

Language: Английский

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The co‐occurrence of two powdery mildew species on Cannabis sativa in the Czech Republic

Journal of Phytopathology, Journal Year: 2024, Volume and Issue: 172(3)

Published: May 1, 2024

Abstract In late summer 2022 and 2023, powdery mildew symptoms were found on female plants of hemp ( Cannabis sativa ) growing in a private garden Hradec nad Moravicí (Silesia, Czech Republic). On the upper sides leaves, white colonies observed which coalesced finally covered whole leaf surface. The stems flowers not affected. Microscopic examination revealed formation asexual (anamorphic) states characterized by conidiophores forming catenescent conidia (Euoidium type), but with two types conidia, viz., ellipsoid to ovoid or doliiform fibrosin bodies (resembling Podosphaera spp.), without Golovinomyces spp.). Molecular genetic analyses confirmed co‐infections studied samples species, macularis ambrosiae , represent first records from Republic, as well Europe world. authors discuss latest information ideas regarding this pathosystem.

Language: Английский

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