Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (Vitis vinifera L.) Under Abiotic Stress and During Development

Plants, Journal Year: 2025, Volume and Issue: 14(1), P. 128 - 128

Published: Jan. 4, 2025

Amino acids in wine grapes function as precursors for various secondary metabolites and play a vital role plant growth, development, stress resistance. The amino acid/auxin permease (AAAP) genes encode large family of transporters; however, the identification AAAP gene remain limited. Consequently, we conducted comprehensive bioinformatics analysis all grapes, encompassing genome sequence analysis, conserved protein domain identification, chromosomal localization, phylogenetic relationship expression profiling. This study identified 60 VvAAAP genes, distributed on 14 chromosomes classified into eight subfamilies. Microarray transcriptome data revealed that most decrease during but VvAAAP7 VvAAAP33 gradually increase. VvAAAP23 VvAAAP46 exhibited significantly higher levels, while VvAAAP30 demonstrated lower when subjected to salt drought stress. diverse patterns, suggesting possesses both diversity specific functions grapes. Furthermore, patterns analyzed by RT-qPCR facilitate further investigation biological individual different tissues. These findings provide valuable insights continued family’s

Language: Английский

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Genome-Wide Identification of the Shaker Potassium Channel Family in Chinese Cabbage and Functional Studies of BrKAT1 in Yeast

Agronomy, Journal Year: 2024, Volume and Issue: 14(9), P. 1954 - 1954

Published: Aug. 29, 2024

Shaker potassium channels play a crucial role in (K+) nutrition and stress resistance plants. However, systematic research on K+ Chinese cabbage [Brassica rapa var. chinensis (L.) Kitamura] remains scarce. This study identified 13 channel members within the genome, which are unevenly distributed across eight chromosomes. Notably, number of exceeds that found model plants Arabidopsis (9) rice (10). discrepancy is attributed to higher homologous proteins Groups II V cabbage, with gene segmental duplication these two subgroups being significant factor contributing expansion family. Interspecies collinearity analysis revealed whole genome family show greater similarity those than rice, indicating from Brassicaceae have closer relationship Poaceae Given occurs Group II, we investigated whether functional difference exists between BrKAT1.1 BrKAT1.2 using yeast assays promoter analysis. The expression BrKAT1 genes uptake-deficient mutant R5421 can restore growth under low conditions, their absorption. Truncation N-terminal 63 amino acids resulted loss absorption capability, suggesting N-terminus essential for maintaining function BrKAT1.2. Furthermore, salt-sensitive G19 enhances tolerance salt stress. These results demonstrate exhibit similar abilities uptake tolerance. lay regulatory elements, differences transcriptional regulation contributed differentiation findings provide foundation understanding evolution mechanisms improving this species through manipulation BrKAT1.

Language: Английский

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Genome-Wide Identification and Expression Analysis of the Cyclic Nucleotide-Gated Channel Gene Family in Zoysia japonica under Salt Stress

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10114 - 10114

Published: Sept. 20, 2024

Salt stress severely inhibits plant growth. Understanding the mechanism of salt tolerance is highly important to improving tolerance. Previous studies have shown that nonselective cyclic nucleotide-gated ion channels (CNGCs) play an role in However, current research on CNGCs mainly focuses glycophytic plants, and halophytes exhibit special strategies still scarce. This study used halophilic Zoysia japonica, excellent warm-season turfgrass, as experimental material. Through bioinformatics analysis, 18 members CNGC family were identified japonica; they designated ZjCNGC1 through ZjCNGC18 according their scaffold-level chromosomal positions. ZjCNGCs are divided into four groups (I–IV), with same having differentiated protein-conserved domains gene structures. unevenly distributed 16 chromosomes. Compared other species, Group III obvious expansion, due duplication segments. The collinearity between ZjCNGCs, OsCNGCs, SjCNGCs suggests evolutionarily conserved among gramineous plants. only partially collinear OsCNGCs SjCNGCs, implying expansion ZjCNGC genes may been independent event occurring japonica. Protein interaction prediction revealed calcium-dependent protein kinase, H+-ATPase, outwardly rectifying potassium channel protein, polyubiquitin 3 interact ZjCNGCs. Multiple response regulatory elements, including those involved stress, present promoter. qPCR results differences expression patterns different parts plant. Under conditions, was significantly upregulated roots leaves, ZjCNGC8 ZjCNGC13 showing greatest increase roots. These collectively suggest be a underlying

Language: Английский

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Cullin-Conciliated Regulation of Plant Immune Responses: Implications for Sustainable Crop Protection

Plants, Journal Year: 2024, Volume and Issue: 13(21), P. 2997 - 2997

Published: Oct. 26, 2024

Cullins are crucial components of the ubiquitin–proteasome system, playing pivotal roles in regulation protein metabolism. This review provides insight into wide-ranging functions cullins, particularly focusing on their impact plant growth, development, and environmental stress responses. By modulating cullin-mediated mechanisms, researchers can fine-tune hormone-signaling networks to improve various agronomic traits, including architecture, flowering time, fruit nutrient uptake. Furthermore, targeted manipulation cullins that involved pathways, e.g., cytokinin, auxin, gibberellin, abscisic acids, ethylene, boost crop growth development while increasing yield enhancing tolerance. also play important defense mechanisms through regulating defense-associated metabolism, thus boosting resistance pathogens pests. Additionally, this highlights potential integrating cullin-based strategies with advanced biological tools, such as CRISPR/Cas9-mediated genome editing, genetic engineering, marker-associated selections, gene overexpression, knockout, achieve precise modifications for improvement sustainable agriculture, promise creating resilient, high-yielding, environmentally friendly varieties.

Language: Английский

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