Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wilt

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 22, 2025

Thaumatin-like proteins (TLPs) are crucial pathogenesis-related that significantly contribute to plant defense rection. Fusarium oxysporum f. sp. cubense (Foc) causes wilt of bananas, a serious threat global production. However, the role TLPs in disease resistance remains unclear. This study identified 49 TLP genes banana, predominantly localized extracellular space, and distributed across 11 chromosomes. The ancestor–descendant relationship was explained, six remained remarkably conserved species could represent ancestral gene family. Promoter regions, transcriptome qRT-PCR analysis suggested MaTLP16 might be involved resistance. Furthermore, transcriptional silencing resulted more severe leaf damage compared control, indicating is an important Foc resistance-related gene. conducted comprehensive genome-wide identification systematic family bananas. Our findings establish foundation for further functional studies MaTLP highlight as strong candidate use breeding programs aimed at enhancing Musa diseases.

Language: Английский

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The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Feb. 4, 2025

Chickpea (Cicer arietinum L.) ranks as the third most crucial grain legume worldwide. Fusarium wilt (Fusarium oxysporum f. sp. ciceri (Foc)) is a devastating fungal disease that prevents maximum potential for chickpea production. To identify genes and pathways involved in resistance to race 6 of Foc, this study utilized transcriptome sequencing two cultivars: resistant (Ana) susceptible (Hashem) Foc 6. Illumina root samples yielded 133.5 million raw reads, with about 90% clean reads mapped reference genome. The analysis revealed 548 (332 upregulated 216 downregulated) genotype 1115 (595 520 were differentially expressed under (FW) stress caused by expression patterns some (DEGs) validated using quantitative real-time PCR. A total 131 exclusively FW cultivar, including several sensing (e.g., CaNLR-RPM1, CaLYK5-RLK, CaPR5-RLK, CaLRR-RLK, CaRLP-EIX2), signaling CaPP7, CaEPS1, CaSTY13, CaPR-1), transcription regulation CaMYBs, CaGLK, CaERFs, CaZAT11-like, CaNAC6) cell wall integrity CaPGI2-like, CaEXLs, CaCSLD CaCYP73A100-like). achieved results could provide insights into molecular mechanism underlying be valuable breeding programs aimed at developing FW-resistant varieties.

Language: Английский

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A dual transcriptome analysis reveals accession-specific resistance responses in Lathyrus sativus against Erysiphe pisi

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

Lathyrus sativus (grass pea) is a valuable crop for sustainable agriculture, offering dietary benefits and desirable agronomic traits. However, its yield stability limited by diseases such as powdery mildew caused Erysiphe pisi. Increasing fungal resistance to pesticides environmental concerns demand the development of resistant varieties. To identify key defense mechanisms effector genes involved in sativus-Erysiphe pisi interaction we analyzed four L. accessions exhibiting varying E. (resistant, partially resistant, susceptible, susceptible) using dual RNA-Seq experiment across different time points. We observed host biphasic response, characterized an initial burst gene expression, followed quiescent phase, subsequent wave intense expression. Common included antifungal protein cell wall reinforcement, reactive oxygen species-mediated defense. These defenses respectively Bowman-Birk type proteinase inhibitors, peptidyl-prolyl cis-trans isomerases mannitol dehydrogenases. The accession specifically activated early reinforcement structural barriers associated with lignin biosynthesis phenylpropanoid pathway, along sustained chemical (e.g. eugenol synthase 1), epigenetic regulation, oxidative stress responses thorough peroxidases heat shock proteins. partial exhibited front-loaded response at infection stages. Contrastingly, susceptible weaker baseline defense, slower less robust targeting pathogen infection. identified potential effectors, including hydrolysis mannosidase DCW1), nutrient acquisition secreted alpha-glucosidase), virulence SnodProt1), higher diversity effectors accession. In conclusion, this study identifies novel targets NLRs proteins related within complex support future breeding programs aimed enhancing species.

Language: Английский

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Withania somnifera osmotin (WsOsm) confers stress tolerance in tobacco and establishes novel interactions with the defensin protein (WsDF)

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(5)

Published: Sept. 1, 2024

Abstract Pathogenesis‐related proteins (PR), including osmotins, play a vital role in plant defense, being activated response to diverse biotic and abiotic stresses. Despite their significance, the mechanistic insights into of osmotins defense have not been extensively explored. The present study explores cloning characterization osmotin gene ( WsOsm ) from Withania somnifera , aiming illuminate its mechanisms. Quantitative real‐time PCR analysis revealed significant induction various phytohormones e.g. abscisic acid, salicylic methyl jasmonate, brassinosteroids, ethrel, as well stresses like heat, cold, salt, drought. To further elucidate 's functional role, we overexpressed Nicotiana tabacum resulting heightened resistance against Alternaria solani pathogen. Additionally, observed enhancements shoot length, root biomass transgenic tobacco plants compared wild plants. Notably, WsOsm‐ overexpressing seedlings demonstrated improved salt drought stress tolerance, particularly at seedling stage. Confocal histological H 2 O biochemical studies antioxidant enzyme activities higher levels lines, indicating enhanced defense. Furthermore, pull‐down assay mass spectrometry potential interaction between defensin, known antifungal PR protein (WsDF). This suggests novel mediating responses by interacting with other proteins. Overall, these findings pave way for future applications developing stress‐tolerant crops improving strategies pathogens.

Language: Английский

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The module consisting of transcription factor WRKY14 and thaumatin‐like protein TLP25 is involved in winter adaptation in Ammopiptanthus mongolicus

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(5)

Published: Sept. 1, 2024

Abstract Thaumatin‐like proteins (TLPs) are conserved involved in the defense and stress responses of plants. Previous studies showed that several TLPs were accumulated leaf apoplast Ammopiptanthus mongolicus winter, indicating might be related to adaptation winter climate A. . To investigate roles adaptation, we first analyzed expression pattern TLP genes then focused on biological function regulation pathway AmTLP25 gene. Several genes, including , upregulated during response both cold osmotic stress. Overexpression gene led an increased tolerance transgenic Arabidopsis freezing Furthermore, elevated AmWRKY14 transcription factor activated by specifically binding its promoter. It is speculated – module contributes temperate Our research advances current understanding regulatory provides valuable information for molecular mechanism evergreen broad‐leaved plants adapting climate.

Language: Английский

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In Silico Structural and Functional Characterization of the Thaumatin‐Like Gene Family in Phaseolus lunatus Reveals the Potential for Microbial and Antiviral Activity

Peptide Science, Journal Year: 2024, Volume and Issue: 116(6)

Published: July 18, 2024

ABSTRACT Thaumatin‐like proteins (TLPs) are part of the pathogenesis‐related (PR) that important in host plant defense. TLPs have multiple defense functions against pathogens (bacteria, viruses, and fungi) involved other biological processes several living organisms. However, role must be elucidated for species, such as Phaseolus lunatus , popularly known lima bean. In this study, we propose to investigate activity P. which can help develop a detailed functional characterization TLP gene crop cultures. As result, total 31 PlTPLs genes 16 highly conserved cysteine residues each family member were identified bean genome. Regarding phylogenetic analysis, 128 from different including used, phenetic tree revealed could divided into six groups. The leading group was Group 4, containing 34 TLPs; smallest 7, only single TLP. analysis antimicrobial antiviral 27 PlTLPs 7 potential activity. For chromosomal distribution 8 11 chromosomes presented at least one PlTLs on chromosomes. Finally, molecular modeling carried out between two (PlTLP5 PlTLP14), did not allergenic results showed candidates ≥90% their favorable regions ∼10% permitted regions, demonstrating considered promising targets biotechnological applications, especially those related genetic improvement agricultural crops development agents.

Language: Английский

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PbrATG6 modulates reactive oxygen species metabolism and interacts with PbrTLP15 synergistic enhancement of pear resistance to Botryosphaeria dothidea

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 281, P. 136663 - 136663

Published: Oct. 17, 2024

Language: Английский

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Genome-Wide Identification and Stress Responses of Cowpea Thaumatin-like Proteins: A Comprehensive Analysis

Plants, Journal Year: 2024, Volume and Issue: 13(22), P. 3245 - 3245

Published: Nov. 19, 2024

Cowpea (

Language: Английский

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Comparative transcriptomics ofLathyrus sativusreveals accession-specific resistance responses againstErysiphe pisi

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 25, 2024

Lathyrus sativus (grass pea) is a valuable crop for sustainable agriculture, offering both dietary benefits and desirable agronomic traits. However, its yield stability limited by different diseases such as powdery mildew caused Erysiphe pisi . Frequent fungal resistance to pesticides growing environmental concerns highlight the need research investment develop resistant varieties. Four L. accessions, exhibiting varying levels of E. (resistant, partially resistant, susceptible, susceptible), were analysed using dual RNA-seq identify key defence mechanisms effector genes involved in this plant-pathogen interaction. The transcriptomic analysis highlighted host biphasic response, characterised an initial burst gene expression, followed quiescent phase, second wave intense expression at 72 hours after inoculation. Common mechanisms, including antifungal protein cell wall reinforcement, reactive oxygen species-mediated activated all accessions compared susceptible accession. Unique responses accession integrate early reinforcement structural barriers with sustained chemical defences stress responses. Overall, exhibited front-loaded focused on biotic stimuli interspecies interactions infection stages. In contrast, partial weaker baseline system, slower less robust response specifically targeting pathogen infection. We identified potential effectors, hydrolysis, nutrient acquisition, virulence, higher diversity effectors This study identifies novel targets within complex sativus-Erysiphe interaction that will support future breeding programs aimed enhancing other related species.

Language: Английский

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Unraveling the transcriptional response mechanisms to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f.sp. ciceris in two contrasting chickpea cultivars

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Background Chickpea (Cicer arietinum L.) ranks as the third most crucial grain legume worldwide. Fusarium wilt (Fusarium oxysporum f. sp. ciceri (Foc)) is a devastating fungal disease that prevents maximum potential for chickpea production. Results To identify genes and pathways involved in resistance to race 6 of Foc, this study utilized transcriptome sequencing two cultivars: resistant (Ana) susceptible (Hashem) Foc 6. Illumina root samples yielded 133.5 million raw reads, with about 90% clean reads mapped reference genome. The analysis revealed 518 (317 upregulated 201 downregulated) genotype 1063 (587 476 were differentially expressed under (FW) stress caused by expression patterns some (DEGs) validated using quantitative real-time PCR. A total 127 exclusively FW cultivar, including several sensing (e.g., CaNLR-RPM1, CaLYK5-RLK, CaPR5-RLK, CaLRR-RLK, CaRLP-EIX2), signaling CaPP7, CaEPS1, CaSTY13, CaPR-1), transcription regulation CaMYBs, CaGLK, CaERFs, CaZAT11-like, CaNAC6) cell wall integrity CaPGI2-like, CaEXLs, CaCSLD CaCYP73A100-like). Conclusions achieved results could provide significant insights into molecular mechanism underlying be valuable breeding programs aimed at developing FW-resistant varieties.

Language: Английский

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