Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 1, 2024

Introduction Salt stress is a major abiotic that affects crop growth and productivity. Choline Chloride (CC) has been shown to enhance salt tolerance in various crops, but the underlying molecular mechanisms rice remain unclear. Methods To investigate regulatory mechanism of CC-mediated rice, we conducted morpho-physiological, metabolomic, transcriptomic analyses on two varieties (WSY, salt-tolerant, HHZ, salt-sensitive) treated with 500 mg·L -1 CC under 0.3% NaCl stress. Results Our results showed foliar application improved morpho-physiological parameters such as root traits, seedling height, strength index, fullness, leaf area, photosynthetic parameters, pigments, starch, fructose content stress, while decreasing soluble sugar, sucrose, sucrose phosphate synthase levels. Transcriptomic analysis revealed regulation combined treatment induced changes expression genes related starch metabolism, citric acid cycle, carbon sequestration organs, antenna proteins both varieties. Metabolomic further supported these findings, indicating photosynthesis, fixation pathways were crucial tolerance. Discussion The metabolomic data suggest enhances by activating distinct transcriptional cascades phytohormone signaling, along multiple antioxidants unique metabolic pathways. These findings provide basis for understanding metabolite synthesis gene response may inform strategies improving resilience

Language: Английский