Cold plasma: A nonthermal pretreatment, extraction, and solvent activation technique for obtaining bioactive compounds from agro-food industrial biomass

Food Chemistry, Journal Year: 2025, Volume and Issue: 472, P. 142960 - 142960

Published: Jan. 18, 2025

Language: Английский

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Advancing chronic and acute wound healing with cold atmospheric plasma: cellular and molecular mechanisms, benefits, risks, and future directions

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 26, 2025

Chronic and acute wounds represent significant challenges in healthcare, often leading to prolonged recovery times increased complications. While chronic wounds, such as diabetic foot ulcers venous leg ulcers, persist due underlying conditions biofilm formation, including surgical incisions burns, can also benefit from innovative therapeutic approaches. Cold atmospheric plasma (CAP) has emerged a promising non-invasive therapy capable of enhancing wound healing outcomes across both types. This review examines the cellular molecular mechanisms by which CAP promotes repair, focusing on its modulation inflammation, stimulation angiogenesis, facilitation tissue remodeling, antimicrobial effects, potentially be used regenerative medicine. generates reactive oxygen nitrogen species that influence key processes, accelerating regeneration while reducing bacterial load preventing formation. Clinical applications have demonstrated efficacy improving metrics for wounds. Despite results, translating into routine clinical practice requires addressing standardizing treatment protocols, assessing long-term safety, developing portable devices. Future research should prioritize optimizing parameters exploring combination therapies maximize potential. Overall, represents safe, effective, versatile modality management, with potential significantly improve patient care.

Language: Английский

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Innovative Therapeutic Approach Targeting Colon Cancer Stem Cells: Transitional Cold Atmospheric Plasma

ACS Omega, Journal Year: 2025, Volume and Issue: 10(12), P. 12109 - 12121

Published: March 6, 2025

Transitional cold atmospheric plasma (TCAP) represents a novel technique for generating remotely from primary source. It consists of partially nonthermal ionized gas mixture containing charged and neutral particles, photons, free radicals. In recent years, TCAP has attracted considerable attention in biomedical applications. order to evaluate colon cancer stem cells' (CCSCs) proliferation, apoptotic induction, inflammatory response, survival, was utilized both directly indirectly this study. Using argon helium gases, continuously delivered two stages during the experiment. For direct state, irradiated onto CCSCs 3 5 min. indirect technique, Matrigel treated with min before introduction cells. vitro assays demonstrated that exposure significantly reduced viability CCSCs; application had greater impacts than argon. Numerous investigations confirmed induction apoptosis, showing groups more cells altered cellular structures controls (****p < 0.0001). A substantial increase Bax/Bcl-2 ratio found by analyzing expression Bax Bcl-2 genes, indicating increased susceptibility apoptosis (*p = 0.0177 ***p 0.0004). The higher efficacy mode further highlighted marker analysis, which showed significant reduction interleukin-6 interleukin-8 TCAP-helium compared TCAP-argon (**p 0.0015 0.0007). Lastly, proliferation test, relies on Ki-67 expression, noteworthy decline all TCAP-treated groups, group exhibiting most robust impact 0.0014). Overall, findings highlight potential TCAP, particularly helium, as promising approach selectively targeting providing insights into its therapeutic mechanisms treatment. therefore, emerges unique strategy applications cell-targeted therapies.

Language: Английский

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Helium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancer

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 27, 2025

Nowadays, cold atmospheric plasma (CAP) technology has developed as an innovative tool for cancer therapy. Although many studies have reported the antitumor effects of in vivo and vitro, there are challenges, including standardization devices treatment time different tumors. For first time, we aimed to evaluate compare optimal exposure direction-dependent cellular helium argon on 4T1 cells a triple-negative mouse model breast cancer. This study used two types jet with input parameters. In vitro evaluations cell line using MTT assays flow cytometry analysis demonstrate CAP-induced apoptosis all treated groups, especially direct approach. These changes were concurrent increased intracellular reactive oxygen species levels decreased total antioxidant capacity these cells. results revealed that CAP therapy reduces tumor size, decreases Nottingham histological score, prevents weight loss, increases survival rate groups. suggest may overcome adverse approved therapeutic strategies seems be significant issue patients clinical stage, alone or combination current programs.

Language: Английский

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The efficacy of helium generated cold atmospheric plasma for skin disinfection

Journal of Taibah University for Science, Journal Year: 2025, Volume and Issue: 19(1)

Published: April 17, 2025

Language: Английский

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Killing Two Crises with One Spark: Cold Plasma for Antimicrobial Resistance Mitigation and Wastewater Reuse

Water, Journal Year: 2025, Volume and Issue: 17(8), P. 1218 - 1218

Published: April 18, 2025

Global water scarcity and antimicrobial resistance (AMR) represent two escalating crises that urgently demand integrated effective solutions. While wastewater reuse is increasingly promoted as a strategy to alleviate scarcity, conventional treatment processes often fail eliminate persistent contaminants antibiotic-resistant microorganisms. Cold plasma (CP), non-thermal advanced oxidation process, has demonstrated the strong potential simultaneously inactivate pathogens degrade micropollutants. CP generates diverse mix of reactive oxygen nitrogen species (ROS RNS), well UV photons charged particles, capable breaking down complex inducing irreversible damage microbial cells. Laboratory studies have reported bacterial log reductions ranging from 1 >8–9 log10, with Gram-negative such E. coli Pseudomonas aeruginosa showing higher susceptibility than Gram-positive bacteria. The inactivation endospores mixed-species biofilms also been achieved under optimized conditions. Viral studies, including MS2 bacteriophage norovirus surrogates, >99.99%, exposure times short 0.12 s. further shown capacity antibiotic residues ciprofloxacin sulfamethoxazole by >90% reduce ARGs (e.g., bla, sul, tet) in hospital wastewater. This perspective critically examines mechanisms current applications treatment, identifies operational scalability challenges, outlines research agenda for integrating into future frameworks targeting AMR mitigation sustainable management.

Language: Английский

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Optimized atmospheric air cold plasma seed treatments differentially enhance plant growth and herbivore resistance in soybean (Glycine max) and sorghum-sudangrass (Sorghum x drummondii)

Environmental Technology & Innovation, Journal Year: 2025, Volume and Issue: unknown, P. 104231 - 104231

Published: April 1, 2025

Language: Английский

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Recent Advances in Cold Atmospheric Pressure Plasma for E. coli Decontamination in Food: A Review

Plasma, Journal Year: 2025, Volume and Issue: 8(2), P. 18 - 18

Published: May 7, 2025

Cold atmospheric plasma (CAP) acts as a powerful antibacterial tool in the food industry, effectively eliminating E. coli and wide range of pathogens, including bacteria, viruses, fungi, spores, biofilms meat vegetables. Unlike traditional bactericidal methods, CAP leverages an arsenal reactive species, oxygen species (ROS) such ozone (O3) hydroxyl radicals (OH•), nitrogen (RNS) like nitric oxide (NO•), alongside UV radiation charged particles. These agents synergistically dismantle coli’s cell membranes, proteins, DNA, achieving high degradation rates without thermal or chemical damage to processed food. This non-thermal, eco-friendly technology preserves food’s nutritional sensory integrity, offering transformative edge over conventional approaches. It emphasizes critical need optimize treatment parameters (exposure time, gas composition, power) unlock CAP’s full potential. review explores effectiveness degrading coli, emphasizing optimization for practical industry applications its potential scalable safety solution. is crucial conduct further studies enhance implementation, establishing fundamental element advanced processing technologies key measure protecting public health.

Language: Английский

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Combining Cold Atmospheric Plasma and Environmental Nanoparticle Removal Device Reduces Neurodegenerative Markers

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12986 - 12986

Published: Dec. 3, 2024

Ageing leads to a gradual deterioration of the organs, with brain being particularly susceptible, often leading neurodegeneration. This process includes well-known changes such as tau hyperphosphorylation and beta-amyloid deposition, which are commonly associated neurodegenerative diseases but also present in ageing. These structures triggered by earlier cellular energy depletion impaired protein synthesis, both essential for cell function. may part be induced environmental pollution, has been shown accelerate these processes. Cold Atmospheric Plasma (CAP) or atmospheric pressure gas discharge plasmas have promise activating immune system improving function vitro, although their effects at organ level remain poorly understood. Our aim this work is investigate effect device that combines CAP treatment effective removal nanoparticles, typical products on activity aged mouse brains. The results showed an increase capacity, reduction reticulum stress activation autophagic clearance, minimising aggresomes brain. key markers neurodegeneration demonstrating efficacy tested product level.

Language: Английский

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Synergistic Antioxidant Effects of Molecular Hydrogen and Cold Atmospheric Plasma in Enhancing Mesenchymal Stem Cell Therapy

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1584 - 1584

Published: Dec. 23, 2024

In regenerative medicine, mesenchymal stem cells (MSCs) have shown their importance and potential in tissue reconstruction immune system modification. However, such cells’ is often diminished by factors as oxidative stress, rejection, inadequate engraftment. This review highlights the role of molecular hydrogen (H2) cold atmospheric plasma (CAP) adjunct therapies to improve effectiveness MSC therapy. H2 has strong antioxidative anti-inflammatory actions it quenches reactive oxygen species positively stimulates Nrf2 pathway that promotes survival life. CAP, being a modulated source ROS RNS, also assists MSCs altering cellular redox balance, thus facilitating adaptation, migration, differentiation. CAP conjunction with each other assist establishing an ambience favorable for promoting MSCs’ growth abilities, reduce healing time various pathways wound, neuroprotection, ischemia. Besides these concerns, this covers best administration routes doses together study informs on novel dual method aimed at improving outcome therapy while adding several targets relevant clinical uses concerning therapies. Research future deal bettering protocols so therapeutic benefits can be maximized without long-term implications applications.

Language: Английский

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