Chondroitin Sulphate-Chitosan Based Nanogels Loaded with Naringenin-β-Cyclodextrin Complex as Potential Tool for the Treatment of Diabetic Retinopathy: A Formulation Study

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 907 - 932

Published: Jan. 1, 2025

The main purpose of the study was formulation development nanogels (NHs) composed chondroitin sulfate (CS) and low molecular weight chitosan (lCH), loaded with a naringenin-β-cyclodextrin complex (NAR/β-CD), as potential treatment for early-stage diabetic retinopathy. Different formulations NHs were prepared by varying polymer concentration, lCH ratio, pH and, then, characterized particle size, zeta potential, concentration (particles/mL) morphology. Cytotoxicity internalization assessed in vitro using Human Umbilical Vein Endothelial Cells (HUVEC). NAR/β-CD evaluated morphology, complexation efficiency, solubility. Finally, most promising NH prototype (NH@NAR/β-CD) further encapsulation loading capacity, opacity cytotoxicity on HUVEC; release test DPPH assay performed to investigate capability sustain NAR NH@NAR/β-CD antioxidant properties, respectively. properties influenced pH. N3 (0.5 mg/mL; lCH=1.5:1; = 5) N9 lCH=1:1; showed optimal characteristics, including small size (<350 nm) positive facilitating cellular uptake. 71% efficiency enhanced Since superior better biocompatibility, NAR/β-CD. N3@NAR/β-CD release, radical scavenging activity biocompatibility finally demonstrated. physico-chemical responsible their cell uptake, suggesting target retinal endothelial cells. high sustained over 72 hours could guarantee maintenance an effective drug at damage site while reducing injection number. Further studies about safety effectiveness intravitreal NHs@NAR/β-CD will be animal model.

Language: Английский

---

Curcumin-Loaded Lipid Nanoparticles: A Promising Antimicrobial Strategy Against Enterococcus faecalis in Endodontic Infections

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 108 - 108

Published: Jan. 14, 2025

Background/Objectives: This study aims to evaluate the efficacy of curcumin (CUR), a natural polyphenol with potent antimicrobial and anti-inflammatory properties, when formulated as solid lipid nanoparticles (CUR-loaded SLN) against Enterococcus faecalis. Methods: Solid (SLNs) were prepared carrier for CUR, which significantly improved its solubility. SLNs made cetyl palmitate Tween 80 obtained via hot ultrasonication method. The physicochemical properties CUR-loaded evaluated, including their size, stability, release profile. Antimicrobial testing was conducted both sessile planktonic E. faecalis populations. Cytotoxicity assessed on human gingival fibroblasts. Results: exhibited about 200 nm −25 mV surface potential, encapsulation CUR did not affect SLNs. CURs released from in controlled sustained manner over 100 h. remained stable at least two months stored 4 °C or 25 °C, making them suitable clinical use. Antioxidant activity confirmed through DPPH ABTS assays. Free reduced CFU counts by approximately 65% after 24 h exposure. However, this inhibitory effect diminished longer exposure times (48 72 h). studies showed dose- time-dependent effects, 2.5–10 µg/mL range, populations, good cytocompatibility fibroblasts up 2.5 μg/mL, suggesting low toxicity. Conclusions: demonstrate significant faecalis, along cytocompatibility, indicating potential an effective adjunct therapy endodontic treatments.

Language: Английский

---

Formulation of DOX-dimer with bi-functionalized chitooligosaccharide for tumor-specific self-boosted drug release and synergistic chemo/chemodynamic therapy

Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 320, P. 121210 - 121210

Published: July 17, 2023

Language: Английский

---

Preparation and Primary Bioactivity Evaluation of Novel Water-Soluble Curcumin-Loaded Polymeric Micelles Fabricated with Chitooligosaccharides and Pluronic F-68

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(10), P. 2497 - 2497

Published: Oct. 20, 2023

Curcumin (CU) is a bioactive compound extracted from turmeric and has various advantages. However, the benefit of CU limited by its low water solubility (11 ng/mL). This research aimed to fabricate water-soluble nano-formulation with chitooligosaccharides (COS) pluronic F-68 (PF) utilizing polymeric micelle method. The optimized curcumin-loaded chitooligosaccharides/pluronic micelles (COSPFCU) exhibited high encapsulation efficiency loading capacity (75.57 ± 2.35% 10.32 0.59%, respectively). hydrodynamic diameter lyophilized COSPFCU was 73.89 11.69 nm polydispersity index below 0.3. could be completely redispersed in showed DPPH scavenging ability. Meanwhile, significantly reduce cytotoxicity RAW 264.7 cells compared native CU. Furthermore, improved inhibition NO release activity at 72.83 2.37% but 33.20 3.41% for CU, concentration cells.

Language: Английский

---

Chitosan-chondroitin sulfate-daidzein nanoconjugate ameliorates glucocorticoid induced osteoporosis in vivo

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 135662 - 135662

Published: Sept. 1, 2024

Language: Английский

---