Injectable Thermosensitive Thiol-Modified NIPAAm-g-Chitosan Hydrogels for Cartilage Regeneration in a Rabbit Osteoarthritis Model DOI Creative Commons
Paula Carmela O. Ching,

Ya-Ching Chang,

Chen-Hsun Weng

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Cartilage tissue has a limited intrinsic capacity for self-healing. Over the decades, researchers have extensively researched methods of cartilage repair, yet some limitations still need to be resolved. Most studies typically evaluate osteochondral regeneration in normal animals. However, traumatic articular defects may eventually result osteoarthritis (OA), and relationship between OA is not independent. Therefore, this study, effect thiol-modified NIPAAm-g-chitosan (TNC) hydrogels containing human adipose-derived mesenchymal stem cells (hADMSCs), with or without etanercept, TNF-α inhibitor, was evaluated monosodium iodoacetate (MIA)-induced rabbit model. TNC hydrogels, suitable lower critical solution temperature (LCST), porous interior microstructures, enhanced mechanical properties, cytotoxicity were synthesized characterized by DSC, SEM, NMR, CCK8 kit. The models established MIA injection knees verified histological examinations cytokine detection IL-1β, IL-6, TNF-α. According macroscopic evaluations, micro-CT analysis, immunohistochemical results repair showed improvement cell-seeded hydrogel groups compared empty defect groups. Furthermore, etanercept effectively promoted first 4 weeks. In models, hADMSCs etanercepts could promising engineering.

Language: Английский

Injectable Thermosensitive Thiol-Modified NIPAAm-g-Chitosan Hydrogels for Cartilage Regeneration in a Rabbit Osteoarthritis Model DOI Creative Commons
Paula Carmela O. Ching,

Ya-Ching Chang,

Chen-Hsun Weng

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Cartilage tissue has a limited intrinsic capacity for self-healing. Over the decades, researchers have extensively researched methods of cartilage repair, yet some limitations still need to be resolved. Most studies typically evaluate osteochondral regeneration in normal animals. However, traumatic articular defects may eventually result osteoarthritis (OA), and relationship between OA is not independent. Therefore, this study, effect thiol-modified NIPAAm-g-chitosan (TNC) hydrogels containing human adipose-derived mesenchymal stem cells (hADMSCs), with or without etanercept, TNF-α inhibitor, was evaluated monosodium iodoacetate (MIA)-induced rabbit model. TNC hydrogels, suitable lower critical solution temperature (LCST), porous interior microstructures, enhanced mechanical properties, cytotoxicity were synthesized characterized by DSC, SEM, NMR, CCK8 kit. The models established MIA injection knees verified histological examinations cytokine detection IL-1β, IL-6, TNF-α. According macroscopic evaluations, micro-CT analysis, immunohistochemical results repair showed improvement cell-seeded hydrogel groups compared empty defect groups. Furthermore, etanercept effectively promoted first 4 weeks. In models, hADMSCs etanercepts could promising engineering.

Language: Английский

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