High-Yield Production of Polyhydroxybutyrate and Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Crude Glycerol by a Newly Isolated Burkholderia Species Oh_219 DOI Open Access

Suk-Jin Oh,

Gaeun Lim,

Ye Sun Han

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(2), P. 197 - 197

Published: Jan. 14, 2025

Crude glycerol (CG), a major biodiesel production by-product, is the focus of ongoing research to convert it into polyhydroxyalkanoate (PHA). However, few bacterial strains are capable efficiently achieving this conversion. Here, 10 PHA-producing were isolated from various media. Among them, Burkholderia sp. Oh_219 exhibited highest polyhydroxybutyrate (PHB) and was therefore characterized further. demonstrated significant tolerance growth inhibitors in CG metabolized fatty acids present as impurities CG. Furthermore, strain genetically engineered using phaCBP-M-CPF4 phaJPa enable acid-based poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), component The resulting produced PHBHHx containing 1.0–1.3 mol% 3HHx Further supplementation with capric lauric increased molar fraction 9.7% 18%, respectively. In 5 L fermenter, 15.3 g/L PHB 29.6 biomass two-stage fermentation system. This yield reported for PHA by spp. Additionally, had lower melting point than that pure fructose. Taken together, promising new candidate producing

Language: Английский

High-Yield Production of Polyhydroxybutyrate and Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Crude Glycerol by a Newly Isolated Burkholderia Species Oh_219 DOI Open Access

Suk-Jin Oh,

Gaeun Lim,

Ye Sun Han

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(2), P. 197 - 197

Published: Jan. 14, 2025

Crude glycerol (CG), a major biodiesel production by-product, is the focus of ongoing research to convert it into polyhydroxyalkanoate (PHA). However, few bacterial strains are capable efficiently achieving this conversion. Here, 10 PHA-producing were isolated from various media. Among them, Burkholderia sp. Oh_219 exhibited highest polyhydroxybutyrate (PHB) and was therefore characterized further. demonstrated significant tolerance growth inhibitors in CG metabolized fatty acids present as impurities CG. Furthermore, strain genetically engineered using phaCBP-M-CPF4 phaJPa enable acid-based poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), component The resulting produced PHBHHx containing 1.0–1.3 mol% 3HHx Further supplementation with capric lauric increased molar fraction 9.7% 18%, respectively. In 5 L fermenter, 15.3 g/L PHB 29.6 biomass two-stage fermentation system. This yield reported for PHA by spp. Additionally, had lower melting point than that pure fructose. Taken together, promising new candidate producing

Language: Английский

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