Interpenetrating Polymer Network of Hydroxyethyl Methacrylate and Chitosan as Cryogels for Tissue Engineering Applications

Polymers for Advanced Technologies, Journal Year: 2025, Volume and Issue: 36(1)

Published: Jan. 1, 2025

ABSTRACT Tissue engineering is a vast expanding field with applications in areas such as tissue/organ transplantation, drug delivery, vitro models, and so on. Biomaterials form an essential component tissue by acting template for cellular activity, therefore, novel tissue‐engineered biomaterials innovative properties are high demand. Hence, this work proposes interpenetrating polymer network cryogel of chitosan hydroxyethyl methacrylate (HEMA) biomaterial uniform cell seeding throughout the matrix. The physical analysis cryogels demonstrated highly macroporous structure exhibiting pore size distribution overall porosity through emission‐scanning electron microscopy (FE‐SEM) sizes lying range 50–200 μm 150–400 horizontal transverse plane, respectively. were also found to be degradable average percent degradation 17.28 ± 1.47% 4 weeks, their mechanical revealed compressive strength 0.05 MPa elastic modulus 3 MPa. Further, biological characterization direct contact test depicted excellent biocompatibility L929 mouse fibroblast MC3T3‐E1 preosteoblasts negligible presence dead cells around cryogel. Uniform increasing proliferation trend was observed on these live‐dead staining MTT assay at day 1, 3, 7‐time point. Cell adherence studies via FE‐SEM similar along flattened morphology extracellular matrix production. Therefore, based promising physico‐chemical properties, HEMA‐Chitosan exhibit strong potential application engineered biomaterials.

Language: Английский

Exploring Biomaterial Scaffolds for Eyelid Reconstruction: A Synthesis of Experimental Findings

Tissue Engineering Part B Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

This review synthesizes experimental findings on various biomaterial scaffolds used in eyelid reconstruction. It examines the structural properties, cellular responses, and functional outcomes of such as chitosan, poly(propylene glycol fumarate)-2-hydroxyethyl methacrylate, fumarate) - type I collagen (PPF-Col), decellularized matrix-polycaprolactone, branched polyethylene, collagen, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate, poly(lactic-co-glycolic acid. These exhibit diverse mechanical biological with some demonstrating good biocompatibility, tunable potential for tissue repair. However, there are limitations, including concerns about long-term functionality a lack comprehensive evaluations. highlights need multifunctional that combine lid replacement ocular surface function restoration, well establishment standardized research methods. The goal is to guide future innovation field improve quality life patients defects.

Language: Английский