Integrating Physical and Biochemical Cues for Muscle Engineering: Scaffolds and Graft Durability DOI Creative Commons

Farbod Yousefi,

Lyndon M. Foster,

Omar Selim

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(12), P. 1245 - 1245

Published: Dec. 9, 2024

Muscle stem cells (MuSCs) are essential for skeletal muscle regeneration, influenced by a complex interplay of mechanical, biochemical, and molecular cues. Properties the extracellular matrix (ECM) such as stiffness alignment guide cell fate through mechanosensitive pathways, where forces like shear stress translate into biochemical signals, affecting behavior. Aging introduces senescence which disrupts MuSC niche, leading to reduced regenerative capacity via epigenetic alterations metabolic shifts. Transplantation further challenges viability, often resulting in fibrosis driven dysregulated fibro-adipogenic progenitors (FAPs). Addressing these issues, scaffold designs integrated with pharmacotherapy emulate ECM environments, providing cues that enhance graft functionality endurance. These scaffolds facilitate synergy between mechanotransduction intracellular signaling, optimizing proliferation differentiation. Innovations utilizing human pluripotent cell-derived myogenic exosome-mediated delivery exploit bioactive properties targeted repair. Additionally, 3D-printed electrospun adjustable biomechanical traits tackle scalability treating volumetric loss. Advanced techniques single-cell RNA sequencing high-resolution imaging unravel repair mechanisms, offering precise mapping cellular interactions. Collectively, this interdisciplinary approach fortifies tissue durability maintenance, propelling therapeutic strategies injuries degenerative diseases.

Language: Английский

The critical role of nano-hydroxyapatites as an advanced scaffold in drug delivery towards efficient bone regeneration: Recent progress and challenges DOI Creative Commons

Amir Hatami kaleshtari,

Samira Farjaminejad,

Melika Hasani

et al.

Carbohydrate Polymer Technologies and Applications, Journal Year: 2025, Volume and Issue: unknown, P. 100692 - 100692

Published: Jan. 1, 2025

Language: Английский

Citations

2
From Molecules to Mind: The Critical Role of Chitosan, Collagen, Alginate, and Other Biopolymers in Neuroprotection and Neurodegeneration DOI Creative Commons
Weronika Kruczkowska, Julia Gałęziewska,

Katarzyna Grabowska

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1017 - 1017

Published: Feb. 22, 2025

Neurodegenerative disorders present significant therapeutic challenges, particularly due to the complex nature of drug delivery central nervous system. This review investigates applications various biopolymers in neuroprotection and their potential role treating neurodegeneration. We a critical analysis natural synthetic biopolymers, focusing primarily on chitosan, fish collagen/gelatin, alginate as key agents. The examines fundamental mechanisms brain development neurodegeneration, establishing framework for understanding how these interact with neural tissues. By analyzing recent experimental studies, we evaluate effectiveness different biopolymer-based systems crossing blood-brain barrier subsequent neuroprotective effects. Additionally, promising materials, including lignin, poly lactic-co-glycolic acid, glucose-modified bovine serum albumin/procyanidin complexes, are briefly explored provide comprehensive overview current developments field. Our reveals that approaches offer unique advantages both delivery, potentially opening new avenues neurodegenerative conditions. synthesizes knowledge identifies directions future research strategies.

Language: Английский

Citations

0
Optimization of PETG 3D printing parameters for the design and development of biocompatible bone implants DOI Creative Commons
Muhammad Sultan,

Tauseef Aized,

Muhammad Farooq

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 27, 2025

The search for suitable manufacturing methods and the selection of biocompatible material with good mechanical properties is still a major challenge in implant development. polyethylene terephthalate glycol (PETG) thermoplastic extensively utilized biomedical applications, like tissue engineering, dental, scaffolds surgery, because its biocompatibility. Fused deposition modeling (FDM) gaining importance wide range applications developing custom shaped medical implants. This study aimed to fabricate cranial using optimized parameters 3D printed PETG properties. research investigates optimization key printing layer height, line width print speed by utilizing Box Behnken Design (BBD). Analysis suggests that influential FDM are height width, which significantly influence tensile compressive strength. analysis variance (ANOVA) showed 0.12 mm, 0.77 mm 25.75 mm/s indicated increased value strength, i.e., 51.18 MPa 52.33 MPa, respectively. effectiveness RSM model was confirmed validation experiment, errors less than 2%. Additionally, this presents process framework development customized implants computed tomography (CT) scan data patient. tested under uniaxial load shows an average peak 1088 N. goal assist surgeons overcoming clinical challenges faced while selecting materials in-house production patient-specific A further evaluation presented technology recommended potential use trials.

Language: Английский

Citations

0
Injectable Biopolymer-Based Hydrogels: A Next-Generation Platform for Minimally Invasive Therapeutics DOI Creative Commons
Nargish Parvin, Sang Woo Joo, Tapas Kumar Mandal

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(6), P. 383 - 383

Published: May 23, 2025

Injectable biopolymer-based hydrogels have emerged as a powerful class of biomaterials designed for minimally invasive therapeutic strategies in modern medicine. These smart hydrogels, derived from natural biopolymers, such alginate, chitosan, gelatin, hyaluronic acid, and collagen, offer unique advantages, including biocompatibility, biodegradability, the ability to mimic extracellular matrix. This review provides comprehensive overview recent advancements design, crosslinking mechanisms, biofunctionality injectable tailored targeted drug delivery tissue regeneration. Special attention is given their role situ gelling systems, cancer therapy, musculoskeletal repair, neural Challenges related mechanical strength, degradation control, clinical translation are also discussed, along with future perspectives scalable manufacturing regulatory approval.

Language: Английский

Citations

0
Polymer-Based Scaffolds as an Implantable Material in Regenerative Dentistry: A Review DOI Creative Commons

Ľuboš Leško,

Petra Jungová, Martina Culenova

et al.

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(3), P. 80 - 80

Published: Feb. 24, 2025

Polymer-based scaffolds have emerged as transformative materials in regenerative dentistry, enabling the restoration and replacement of dental tissues through tissue engineering approaches. These scaffolds, derived from natural synthetic polymers, mimic extracellular matrix to promote cellular attachment, proliferation, differentiation. Natural polymers such collagen, chitosan, alginate offer biocompatibility bioactivity, while alternatives like polylactic acid (PLA) polycaprolactone (PCL) provide tunable mechanical properties degradation rates. Recent advancements highlight integration bioactive molecules nanotechnology enhance potential these materials. Furthermore, developing hybrid combining addresses strength challenges, paving way for patient-specific treatments. Innovations 3D bioprinting stimuli-responsive biomaterials are expected refine scaffold design further, improving therapeutic precision clinical outcomes. This review underscores critical role polymer-based advancing focusing on their applications, advantages, limitations.

Language: Английский

Citations

0
The Role of Tissue Engineering in Orthodontic and Orthognathic Treatment: A Narrative Review DOI Creative Commons
Rosana Farjaminejad, Samira Farjaminejad,

Melika Hasani

et al.

Oral, Journal Year: 2025, Volume and Issue: 5(1), P. 21 - 21

Published: March 20, 2025

Background: Orthodontics and orthognathic surgery present challenges such as extended treatment durations, patient discomfort, complications like root resorption. Recent advancements in tissue engineering nanotechnology offer promising solutions by improving bone regeneration, periodontal repair, biomaterial integration. Objectives: This review explores the integration of scaffold-based orthodontics, focusing on their roles accelerating reducing times, minimizing adverse effects to enhance predictability success orthodontic interventions. Methods: Relevant literature was selected from PubMed, Scopus, Web Science, studies related scaffold technology, biomaterials, orthodontics. Keywords included “tissue engineering”, “orthodontics”, “biomaterials”, “scaffolds”, “nanotechnology”, “bone regeneration”. Priority given peer-reviewed original studies, systematic reviews, meta-analyses addressing innovative approaches clinical outcomes. Results: Findings indicate that scaffolds regeneration while nanoparticles improve drug delivery efficiency. These contribute faster, more predictable treatments with reduced complications. However, high costs, regulatory hurdles, need for long-term validation remain barriers widespread adoption. Conclusions: Tissue minimally invasive, biologically driven treatment. While significant progress has been made, further cost-effective strategies, approvals are needed integrate these innovations into routine practice.

Language: Английский

Citations

0
Advances in bioink-based 3D printed scaffolds: optimizing biocompatibility and mechanical properties for bone regeneration DOI
Pawan Kumar, Jitender Sharma, Ravinder Kumar

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The development of bioink-based 3D-printed scaffolds has revolutionized bone tissue engineering (BTE) by enabling patient-specific and biomimetic constructs for regeneration.

Language: Английский

Citations

0
Efficacy of Three-Dimensional Bioactive Composites in Long Bone Repair with Photobiomodulation DOI Open Access

Santos Júnior,

Letícia Carlucci dos Santos,

Daniela Vieira Buchaim

et al.

Materials, Journal Year: 2025, Volume and Issue: 18(8), P. 1704 - 1704

Published: April 9, 2025

Different treatments have been proposed for morphofunctional bone repair; however, they are not always efficient and limitations. In this experimental study, we present matrix composites with a possible synergistic effect acting scaffolds growth use of photobiomodulation (PBM) to accelerate tissue repair. Thus, the objective was evaluate PBM in repair long (tibia) rats filled biomimetic collagen matrices nanohydroxyapatite heterologous fibrin biopolymer (FB). Forty-eight were distributed into eight groups (n = six each): Blood Clot (BC), + (BCP), Matrix (M), (MP), Fibrin Biopolymer (FB), (FBP), FB (MFB), (MFBP). A 2.0 mm defect created proximal third left tibia. The BCP, MP, FBP, MFBP underwent during surgery maintained twice week until euthanasia at 42 days. Microcomputed tomography (micro-CT), histomorphological histomorphometric analyses performed. Micro-CT analysis revealed that influenced cortical interposition between remnant newly formed bone. Histologically, no exacerbated inflammatory focus or foreign body-type granulomatous reaction observed any group; vast collagenous more oriented thicker spatial conformation PBM-treated groups. Histomorphometrically, showed significantly higher values compared other Specifically, BC group presented mean density 68.33 ± 7.394, while BCP MP 99.83 11.87 99.67 20.58, respectively (p < 0.05). Qualitative fibers indicated enhanced organization maturation This study concluded association bones rats, biopolymer, results contribute improvement growth, together scaffolds.

Language: Английский

Citations

0
3D Printing and Electrospinning of Drug- and Graphene-Enhanced Polycaprolactone Scaffolds for Osteochondral Nasal Repair DOI Open Access
Izabella Rajzer, Anna Kurowska, Anna Nikodem

et al.

Materials, Journal Year: 2025, Volume and Issue: 18(8), P. 1826 - 1826

Published: April 16, 2025

A novel bi-layered scaffold, obtained via 3D printing and electrospinning, was designed to improve osteochondral region reconstruction. The upper electrospun membrane will act as a barrier against unwanted tissue infiltration, while the lower 3D-printed layer provide porous structure for ingrowth. Graphene integrated into scaffold its antibacterial properties, drug Osteogenon® (OST) added promote bone regeneration. composite scaffolds were subjected comprehensive physical, thermal, mechanical evaluations. Additionally, their biological functionality assessed by means of NHAC-kn cells. 0.5% graphene addition PCL significantly increased strain at break, enhancing material ductility. GNP also acted an effective nucleating agent, raising crystallization temperatures supporting mineralization. high surface area facilitated rapid apatite formation attracting calcium phosphate ions. This confirmed FTIR, µCT SEM analyses, which highlighted positive impact on mineral deposition. synergistic interaction between nanoplatelets created bioactive environment that enhanced cell adhesion proliferation, promoted superior formation. These findings highlight scaffold’s potential promising biomaterial repair regenerative medicine.

Language: Английский

Citations

0
Integrating Physical and Biochemical Cues for Muscle Engineering: Scaffolds and Graft Durability DOI Creative Commons

Farbod Yousefi,

Lyndon M. Foster,

Omar Selim

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(12), P. 1245 - 1245

Published: Dec. 9, 2024

Muscle stem cells (MuSCs) are essential for skeletal muscle regeneration, influenced by a complex interplay of mechanical, biochemical, and molecular cues. Properties the extracellular matrix (ECM) such as stiffness alignment guide cell fate through mechanosensitive pathways, where forces like shear stress translate into biochemical signals, affecting behavior. Aging introduces senescence which disrupts MuSC niche, leading to reduced regenerative capacity via epigenetic alterations metabolic shifts. Transplantation further challenges viability, often resulting in fibrosis driven dysregulated fibro-adipogenic progenitors (FAPs). Addressing these issues, scaffold designs integrated with pharmacotherapy emulate ECM environments, providing cues that enhance graft functionality endurance. These scaffolds facilitate synergy between mechanotransduction intracellular signaling, optimizing proliferation differentiation. Innovations utilizing human pluripotent cell-derived myogenic exosome-mediated delivery exploit bioactive properties targeted repair. Additionally, 3D-printed electrospun adjustable biomechanical traits tackle scalability treating volumetric loss. Advanced techniques single-cell RNA sequencing high-resolution imaging unravel repair mechanisms, offering precise mapping cellular interactions. Collectively, this interdisciplinary approach fortifies tissue durability maintenance, propelling therapeutic strategies injuries degenerative diseases.

Language: Английский

Citations

0