Enhanced Nanogel Formulation Combining the Natural Photosensitizer Curcumin and Pectis brevipedunculata (Asteraceae) Essential Oil for Synergistic Daylight Photodynamic Therapy in Leishmaniasis Treatment DOI Creative Commons
Loretta Gasco,

Estela Mesquita Marques,

Daniele Stéfanie Sara Lopes Lera‐Nonose

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 286 - 286

Published: Feb. 21, 2025

Background/Objectives: Neglected tropical diseases (NTDs), such as leishmaniasis, remain a global health challenge due to limited therapeutic options and rising drug resistance. In this study, we developed an advanced nanogel formulation incorporating curcumin (CUR) Pectis brevipedunculata essential oil (EOPb) within F127/Carbopol 974P matrix enhance bioavailability efficacy against Leishmania (Leishmania) amazonensis (LLa) promastigotes. Methods: The chemical profile of EOPb was determined through GC-MS NMR analyses, confirming the presence key bioactive monoterpenes neral, geranial, α-pinene, limonene. (nGPC) optimized ensure thermosensitivity, stability, exhibiting sol–gel transition at physiological temperatures. Rheological analysis revealed that nGPC exhibited Newtonian behavior 5 °C, transitioning shear-thinning thixotropic characteristics 25 32 respectively. This facilitates its application controlled release, making it ideal for topical formulations. Dynamic light scattering (DLS) demonstrated maintained stable nanoscale structure with hydrodynamic radius below 300 nm, while Fourier-transform infrared spectroscopy (FTIR) confirmed strong molecular interactions between EOPb, CUR, polymer matrix. Biological assays significantly enhanced anti-promastigote activity compared free CUR OEPb. Results: At highest tested concentration (50 μg/mL 17.5 CUR) induced over 88% mortality in LLa promastigotes across 24, 48, 72 h, indicating sustained efficacy. Even lower concentrations, retained dose-dependent activity, suggesting synergistic effect EOPb. These findings highlight potential innovative nanocarrier daylight photodynamic therapy (dPDT) treatment leishmaniasis. Future studies will investigate underlying mechanisms synergism explore (PDT) further outcomes.

Language: Английский

Enhanced Nanogel Formulation Combining the Natural Photosensitizer Curcumin and Pectis brevipedunculata (Asteraceae) Essential Oil for Synergistic Daylight Photodynamic Therapy in Leishmaniasis Treatment DOI Creative Commons
Loretta Gasco,

Estela Mesquita Marques,

Daniele Stéfanie Sara Lopes Lera‐Nonose

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 286 - 286

Published: Feb. 21, 2025

Background/Objectives: Neglected tropical diseases (NTDs), such as leishmaniasis, remain a global health challenge due to limited therapeutic options and rising drug resistance. In this study, we developed an advanced nanogel formulation incorporating curcumin (CUR) Pectis brevipedunculata essential oil (EOPb) within F127/Carbopol 974P matrix enhance bioavailability efficacy against Leishmania (Leishmania) amazonensis (LLa) promastigotes. Methods: The chemical profile of EOPb was determined through GC-MS NMR analyses, confirming the presence key bioactive monoterpenes neral, geranial, α-pinene, limonene. (nGPC) optimized ensure thermosensitivity, stability, exhibiting sol–gel transition at physiological temperatures. Rheological analysis revealed that nGPC exhibited Newtonian behavior 5 °C, transitioning shear-thinning thixotropic characteristics 25 32 respectively. This facilitates its application controlled release, making it ideal for topical formulations. Dynamic light scattering (DLS) demonstrated maintained stable nanoscale structure with hydrodynamic radius below 300 nm, while Fourier-transform infrared spectroscopy (FTIR) confirmed strong molecular interactions between EOPb, CUR, polymer matrix. Biological assays significantly enhanced anti-promastigote activity compared free CUR OEPb. Results: At highest tested concentration (50 μg/mL 17.5 CUR) induced over 88% mortality in LLa promastigotes across 24, 48, 72 h, indicating sustained efficacy. Even lower concentrations, retained dose-dependent activity, suggesting synergistic effect EOPb. These findings highlight potential innovative nanocarrier daylight photodynamic therapy (dPDT) treatment leishmaniasis. Future studies will investigate underlying mechanisms synergism explore (PDT) further outcomes.

Language: Английский

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