EN PRENSA. High Performance Liquid Chromatography coupled Diode Array Detector (HPLC-DAD) method development and validation for the quantification of triclosan in toothpaste. EN PRENSA DOI Creative Commons
Silvia G. Ceballos‐Magaña, Roberto Muñiz‐Valencia, Martha A. Rodríguez‐Pérez

et al.

Revista Bio Ciencias, Journal Year: 2023, Volume and Issue: 10

Published: Feb. 21, 2023

Triclosan (TCS) is used in many health care products as a broad-spectrum antimicrobial and disinfectant agent; however, it currently considered an emerging pollutant. The present study aimed to develop analytical HPLC method coupled Diode Array Detector for the TCS determination toothpaste. Method development involved optimization of separation sample preparation. For chromatographic optimization, different RP columns aqueous mobile phases were addressed. optimized was achieved around 3 minutes using Symmetry C18 column at 30 °C, 0.6 mL/min flow-rate, 225 nm wavelength detection. toothpaste preparation response surface methodology Box-Behnken fractional factorial design chosen. treatment consisted leaching 0.2 g by sonication 7 2 mL MeOH. According International Conference on Harmonization (ICH, 1996/2005) European Commission Decision 200/657/EC guidelines, developed validated. found fast, economic, linear, sensitive, precise, robust allowed quantify healthcare products.

Language: Английский

Preparation of Ibuprofen-Loaded Inhalable γCD-MOFs by Freeze-Drying Using the QbD Approach DOI Creative Commons

Anett Motzwickler-Németh,

Petra Party,

P Simon

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(11), P. 1361 - 1361

Published: Oct. 24, 2024

Research on cyclodextrin-based metal-organic frameworks (CD-MOFs) is still in its infancy, but their potential for use drug delivery-expressly the lung-seems promising. We aimed to freeze-drying method create a novel approach preparing CD-MOFs. MOFs consisting of γ-cyclodextrin (γCD) and potassium cations (K

Language: Английский

Citations

1
Chemometrics‐Empowered Optimization of a Reliable Reversed Phase–High Performance Liquid Chromatography Method for the Simultaneous Estimation of Erythromycin and Adapalene in Pharmaceutical Nanoformulation DOI
Nisha Shirkoli, Vinayak Mastiholimath

Separation Science Plus, Journal Year: 2024, Volume and Issue: 7(10)

Published: Aug. 6, 2024

ABSTRACT An innovative, simple, economical, and sensitive reversed phase–high performance liquid chromatography (RP–HPLC) chromatographic method for simultaneous analysis of erythromycin (ERY) adapalene (ADP) in bulk pharmaceutical nanoformulation was developed by analytical quality‐by‐design (AQbD) avenue. According to published research, there is no strategy that integrates RP–HPLC with AQbD estimating ERY ADP. The carried out Box–Behnken design, which reduced trial runs design points. Acetonitrile: orthophosphoric acid (OPA) (0.1% triethanolamine) pH 6.2 water used as the mobile phase. ratio set 35:65 (v/v) at 0.8 mL/min flow rate detect analytes 231 nm. As per guidelines International Conference on Harmonization Q1A (R2), approach validated. ADP regression coefficients ( r 2 ) were found be 0.9998 0.9999, respectively, over 5–25 µg/mL range. Validation parameters comprising linearity, sensitivity, system suitability, precision, robustness, accuracy performed. To assess stability, drugs subjected stress conditions (hydrolytic, oxidative, thermal, photolytic), most vulnerable degrade alkaline oxidative conditions. A new technique successfully analyzed novel drug carriers loaded

Language: Английский

Citations

0
​Statistical-based optimization and mechanism assessments of Arsenic (III)​ adsorption by ZnO-Halloysite nanocomposite​ DOI Creative Commons

Mohammad Ali Khoddam,

Reza Norouzbeigi, Elmira Velayi

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 16, 2024

Arsenic contamination in aqueous media is a serious environmental problem, especially developing countries. In this research, the Box-Behnken response surface methodology was used to optimize most relevant variables affecting arsenic adsorption on ZnO-halloysite surface, including temperature, adsorbent dosage, pH, contact time, and As (III) initial concentration. The regression analysis indicated that experimental data were appropriately fitted quadratic model with adjusted R-squared value (R

Language: Английский

Citations

0
Hydroxyapatite/Chondroitin Sulphate Nanocomposite Infused with Black Seed Oil for Bone Tissue Applications: Physicochemical Characterizations and In Vitro Screening DOI

Tooba Fatima,

Reshma Jolly,

Fariha Mushahid

et al.

ChemistrySelect, Journal Year: 2024, Volume and Issue: 9(42)

Published: Nov. 1, 2024

Abstract Morbidities associated with natural bone have highlighted the need of advanced bioactive implants for replacement damaged bone. Known their beneficial bioactivity and biocompatibility, Nigella sativa seeds ( kalonji /black seed) are traditionally used in many medications. This research focuses on positive impact black seed extract chondroitin sulfate‐based hydroxyapatite nanocomposite tissue regeneration applications. Novel nanocomposites varied concentrations (0, 1, 2, 3 wt%) were fabricated through a coprecipitation approach analyzed physicochemical biological The results X‐ray diffraction FTIR spectroscopy revealed that HCSK3 was successfully developed appreciably strong molecular interactions among components. exhibits higher rough surface morphology R −1.13 µm z −4.8 µm), uniform particle distribution (average size −9.9 nm), good thermal stability total weight loss 13%. It also provides better nucleation sites apatite formation protein adsorption stimulating cell attachment, proliferation, differentiation. Moreover, showed excellent viability, ARS/ALP activities, antibacterial property, nonhemolytic property (below 3%). Thus, it can be concluded manifested highest biocompatibility negligible toxicity. Therefore, employed as an ideal implant restoration phenomenon.

Language: Английский

Citations

0
Optimization of HPLC Conditions for Analysis of Paracetamol in Jamu Pegal Linu in Pasar Besar Malang City Using Response Surface Methodology-Box-Behnken Design (RSM-BBD) DOI Creative Commons

Laffael Kevin Immanuel,

Anggita Rosiana Putri, Bachtiar Rifai Pratita Ihsan

et al.

Malaysian Journal of Science and Advanced Technology, Journal Year: 2024, Volume and Issue: unknown, P. 440 - 447

Published: Sept. 29, 2024

Based on the Indonesian Ministry of Health Regulation No.7 in 2012, herbal medicine is disallowed to contain synthetic chemicals or isolated products with medicinal properties. However, from findings BPOM, there are still many producers who add medicine, one which addition paracetamol jamu pegal linu. This study aimed obtain optimum HPLC conditions for analysis linu using Response Surface Methodology-Box-Behnken Design, ensure method has met validation parameters, and determine presence levels contained sold Pasar Besar, Malang City. that were optimized percentage methanol mobile phase water, flow rate, column temperature. The condition optimization results obtained at a water 34.4%, rate 1 mL/min, temperature 30°C. Analysis content test parameters including selectivity (λmax 245 nm; retention time ± 2.6 minutes; resolution 2.5), linearity (r2 = 0.9984), LOD (2.44 ppm), LOQ (7.40 accuracy (97-102%), precision (0.26-0.69%). three samples analyzed showed negative fulfilled regulation 2012.

Language: Английский

Citations

0
The Box–Behnken Design for Optimizing HPLC Separation and Validation of Astilbin in Lysiphyllum strychnifolium Stems DOI Creative Commons
Chaowalit Monton, Jirapornchai Suksaeree

Scientifica, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The goal of the research was to use BBD, a productive RSM approach, enhance HPLC separation and validation astilbin in

Language: Английский

Citations

0
EN PRENSA. High Performance Liquid Chromatography coupled Diode Array Detector (HPLC-DAD) method development and validation for the quantification of triclosan in toothpaste. EN PRENSA DOI Creative Commons
Silvia G. Ceballos‐Magaña, Roberto Muñiz‐Valencia, Martha A. Rodríguez‐Pérez

et al.

Revista Bio Ciencias, Journal Year: 2023, Volume and Issue: 10

Published: Feb. 21, 2023

Triclosan (TCS) is used in many health care products as a broad-spectrum antimicrobial and disinfectant agent; however, it currently considered an emerging pollutant. The present study aimed to develop analytical HPLC method coupled Diode Array Detector for the TCS determination toothpaste. Method development involved optimization of separation sample preparation. For chromatographic optimization, different RP columns aqueous mobile phases were addressed. optimized was achieved around 3 minutes using Symmetry C18 column at 30 °C, 0.6 mL/min flow-rate, 225 nm wavelength detection. toothpaste preparation response surface methodology Box-Behnken fractional factorial design chosen. treatment consisted leaching 0.2 g by sonication 7 2 mL MeOH. According International Conference on Harmonization (ICH, 1996/2005) European Commission Decision 200/657/EC guidelines, developed validated. found fast, economic, linear, sensitive, precise, robust allowed quantify healthcare products.

Language: Английский

Citations

0