Unravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai

Microbiological Research, Journal Year: 2024, Volume and Issue: 288, P. 127841 - 127841

Published: Aug. 2, 2024

In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against range bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin bacilysin, together some ribosomally-synthesized post-translationally modified peptides (RiPPs) such as plantazolicin, potentially amylocyclicin, bacinapeptin LCI. Reverse genetics further showed specific involvement these compounds antagonistic activity strain. Comparative genomics between five already sequenced B. strains products constitute core metabolome species while RiPPs are more strain-specific. Although lacks key bioactive found velezensis, greenhouse experiments show is able protect tomato maize plants early blight northern leaf caused by Alternaria solani Exserohilum turcicum, respectively, at levels similar or better than velezensis QST713. The reduction foliar diseases, following root application suspension demonstrates can act direct antibiosis inducing plant defence mechanisms. These findings indicate be considered good candidate diseases prevailing tropical regions, encourage research into spectrum activity, requirements conditions needed ensure efficacy.

Language: Английский

Synergistic effects of Rahnella aquatilis and Trichoderma orientale in biocontrol of common bacterial blight in bean

Egyptian Journal of Biological Pest Control, Journal Year: 2025, Volume and Issue: 35(1)

Published: March 7, 2025

Abstract Background Common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Xap), is a major disease affecting bean plants. While biocontrol agents have been explored to manage plant diseases, there limited research on the combined use of multiple for controlling blight. This study aimed evaluate efficacy bacterium Rahnella aquatilis (Ra) and fungus Trichoderma orientale (TO), used singly or in combination, protecting plants against this disease. Results In vitro experiments, R. T. strongly inhibited Xap growth exhibited significant zinc phosphate solubilization abilities. Under greenhouse conditions, treatments with Ra, TO, their combination significantly suppressed incidence, achieving reductions 69, 56, 70%, respectively. Both decreased pathogen load leaf tissues improved seed germination rates, which reached 92 82% Ra respectively, compared 67% infected control. Additionally, treated showed increased fresh dry weights roots shoots untreated Conclusion demonstrates that application an effective eco-friendly strategy common blight beans. These synergistically reduced severity, enhanced growth, germination, emphasizing utility sustainable agriculture.

Language: Английский

Screening of As-Resistant Bacterial Strains from the Bulk Soil and the Rhizosphere of Mycorrhizal Pteris vittata Cultivated in an Industrial Multi-Polluted Site

Soil Systems, Journal Year: 2024, Volume and Issue: 8(3), P. 87 - 87

Published: Aug. 3, 2024

Arsenic (As) contamination poses significant environmental and health concerns globally, particularly in regions with high exposure levels due to anthropogenic activities. As phytoremediation, through the hyperaccumulator fern Pteris vittata, offers a promising approach mitigate arsenic pollution. Bacteria mycorrhizal fungi colonizing P. vittata roots are involved metabolism resistance plant growth promotion under stressful conditions. A total of 45 bacterial strains were isolated from bulk soil rhizosphere growing an industrial As-polluted site. characterized by their plant-beneficial traits, tolerance sodium arsenate arsenite, occurrence As-resistant genes. This study highlights differences between culturable fraction microbiota associated plants soil. Moreover, several showing up 600 mM isolated. All possessed arsC genes, about 70% them showed arrA genes anaerobic respiration pathway. The possible exploitation such strategies devoted assisted phytoremediation importance order develop effective situ strategies.

Language: Английский