Extracellular Lipase from Pseudomonas aeruginosa SB-37: Production by Solid State Fermentation, Immobilization, and Characterization DOI Creative Commons
Titin Haryati, Norman Yoshi Haryono,

Dian Nugraheni

et al.

BULLETIN OF CHEMICAL REACTION ENGINEERING AND CATALYSIS, Journal Year: 2024, Volume and Issue: 19(4), P. 710 - 721

Published: Dec. 21, 2024

Native lipase is still promising as an industrial biocatalyst. This study aimed to investigate the production of native local using solid state fermentation (SSF) methods, immobilization by Ca-alginate entrapment, and characterization based on substrate preferences. To obtain high SSF we optimized type agro-wastes substrates, time, oil induction percentage volume preculture percentage. The optimal condition for via solid-state involved a 7% (v/v) Pseudomonas aeruginosa SB-37, utilizing palm kernel meal substrate, supplemented with 6% (v/w) (soybean oil:tween 80 = 75:25) at 50 °C 24 h. gave lypolitic activity value 2 U/gds (gram dry weight substrates). Since protein profile extracellular has few bands, perform direct crude supernatant. Immobilization entrapment results in loading capacity recovery values 86.84% 148%, respectively. immobilized retained 92% until four batch repetition keep 40% tenth batch. highest hydrolytic was 0.9 U/g gel pNP_8 substrates. While transesterification observed acetonitrile solvent substrates isopropanol 0.6 gel. present emphasized feasibility producing biocatalysts economical agro-industrial wastes efficient method. Copyright © 2024 Authors, Published BCREC Publishing Group. open access article under CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Language: Английский

Bioconversion of Liquid and Solid Lipid Waste by Yarrowia lipolytica Yeast: A Study of Extracellular Lipase Biosynthesis and Microbial Lipid Production DOI Creative Commons
Katarzyna Wierzchowska, Karolina Szulc, Bartłomiej Zieniuk

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 959 - 959

Published: Feb. 19, 2025

This study investigated the capabilities of Yarrowia lipolytica strains to grow in media with different hydrophobic wastes from meat industry. The yeast growth, cellular lipid accumulation, production lipases, and degree utilization liquid solid were studied shaken cultures organic inorganic nitrogen sources. effects type waste, initial concentration carbon source, strain, inoculum size two experimental sets using Latin Square 5 × design method. Post-frying rapeseed oil chicken frying was selected as source promote biomass growth. In contrast, fraction broths promoted efficient accumulation lipolytic activity. An at 8% m/v stimulated biosynthesis lipase production, while 2.5% v/v provided optimal conditions for growth substrates. No significant differences observed particle dispersion culture (span = 2.51-3.23). maximum emulsification index (62%) Y. KKP 323 strain medium post-frying frying, which correlated biosurfactant synthesis. It concluded that its structure, composition affected various physiological responses.

Language: Английский

Citations

0
Production and characterization of a promising microbial-derived lipase enzyme targeting BCL-2 gene expression in hepatocellular carcinoma DOI Creative Commons

Amal M. Abo-Kamer,

Ahmed Abdelaziz,

Esraa Sobhy Elkotb

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 8, 2025

Abstract Context and goal This study aimed to isolate optimize a high-yield lipase-producing Pseudomonas aeruginosa strain from biological samples, enhance enzyme production through random mutagenesis, evaluate its potential anticancer activity. Fifty-one samples (blood, urine, sputum, wound pus) were screened, three isolates demonstrated significant lipase The with the highest activity, identified as P. (GenBank accession number PP436388), was subjected ethidium bromide-induced resulting in two-fold increase activity (312 U/ml). Lipase optimized using submerged fermentation, critical factors statistically Tween 80, peptone, substrate concentration. purified via ammonium sulfate precipitation Sephadex G-100 chromatography, molecular weight (53 kDa) confirmed by SDS–PAGE. Findings Optimal conditions for included pH of 9, temperature 20 °C, 24-h incubation period. partially exhibited high stability at values up 10 storage temperatures 4 °C. Anticancer evaluated MTT assay, revealing an IC 50 78.21 U/ml against human hepatocellular carcinoma HepG-2 cells, no cytotoxicity observed Vero cells. Flow cytometry that enzyme’s mediated apoptosis necrosis. QRT-PCR data revealed expression Bcl-2 gene significantly downregulated 62% ( P < 0.05) following treatment cells enzyme. These findings suggest holds promise novel therapeutic agent carcinoma, addressing limitations current treatments.

Language: Английский

Citations

0
Enhanced Lipid Yield from Olive-Mill Wastewater by Yarrowia lipolytica NRRL YB-423 DOI Open Access
Bilge Sayın, Zerrin Polat, Güzin Kaban

et al.

Journal of Agricultural Production, Journal Year: 2025, Volume and Issue: 6(1), P. 32 - 40

Published: March 26, 2025

Lipid production from olive-mill wastewater (OMW) by Yarrowia lipolytica NRRL YB-423 was optimized (biomass concentration and lipid yield based on dry cell weight) using multi-response criteria the Taguchi orthogonal array. Sixteen experimental runs were performed L16 Dilution rates of OMW (15, 30, 45, 60%), Tween 80 (0, 0.2, 0.4, 0.6%), sodium chloride (NaCl; 0, 1, 2, 3%), sterility selected as factors. The significance parameters determined analysis variance (ANOVA). effects all factors statistically significant (p&lt;0.05). results showed that had a maximum contribution 48.12% to yield. highest (40.88 %) achieved in sterile medium supplemented with 15% diluted OMW, 0.6% 80, 3% NaCl.

Language: Английский

Citations

0
Analysis of Human Gut Microbiota Enzymes for Biotechnological and Food Industrial Applications DOI Creative Commons
Alfonso Torres-Sánchez, Gracia Luque, Pilar Ortíz

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(10), P. 1794 - 1794

Published: May 18, 2025

The human gut microbiota is a complex and dynamic ecosystem, recognized for its valuable wide array of physiological functions. This study investigated the as source enzymes innovative applications in biomedicine, bioremediation, food feed biotechnological industries by integrating data from combined silico vitro approaches. A total 93 easily cultivable strains were selected bank isolated microorganisms generated children under different media conditions. First, genomic screening enzyme interrogation reference genomes corresponding to species carried out using custom bioinformatic searching protocol. extraction interpretation encoding taxa results focused on four major phyla (Bacillota, Bacteroidota, Actinomycetota, Pseudomonadota) seven genera (Bacillus, Bacteroides, Clostridium, Enterobacter, Enterococcus, Microbacterium, Staphylococcus) according their cultivability relevance interest. 364 identified across protein annotations, highlighting amylases, cellulases, inulinases, lipases, proteases, laccases. Second, phenotypic assays confirmed these main enzymatic activities 80.6% isolates. Notable findings included Bacillus displaying relevant amylase laccase activity. demonstrates utility combining annotations with functional assays, offering robust approach exploiting develop sustainable processes. Moreover, regulatory mechanisms governing expression resilient microbes are essential steps toward unlocking full potential microbiota-derived biocatalysts.

Language: Английский

Citations

0
Extracellular Lipase from Pseudomonas aeruginosa SB-37: Production by Solid State Fermentation, Immobilization, and Characterization DOI Creative Commons
Titin Haryati, Norman Yoshi Haryono,

Dian Nugraheni

et al.

BULLETIN OF CHEMICAL REACTION ENGINEERING AND CATALYSIS, Journal Year: 2024, Volume and Issue: 19(4), P. 710 - 721

Published: Dec. 21, 2024

Native lipase is still promising as an industrial biocatalyst. This study aimed to investigate the production of native local using solid state fermentation (SSF) methods, immobilization by Ca-alginate entrapment, and characterization based on substrate preferences. To obtain high SSF we optimized type agro-wastes substrates, time, oil induction percentage volume preculture percentage. The optimal condition for via solid-state involved a 7% (v/v) Pseudomonas aeruginosa SB-37, utilizing palm kernel meal substrate, supplemented with 6% (v/w) (soybean oil:tween 80 = 75:25) at 50 °C 24 h. gave lypolitic activity value 2 U/gds (gram dry weight substrates). Since protein profile extracellular has few bands, perform direct crude supernatant. Immobilization entrapment results in loading capacity recovery values 86.84% 148%, respectively. immobilized retained 92% until four batch repetition keep 40% tenth batch. highest hydrolytic was 0.9 U/g gel pNP_8 substrates. While transesterification observed acetonitrile solvent substrates isopropanol 0.6 gel. present emphasized feasibility producing biocatalysts economical agro-industrial wastes efficient method. Copyright © 2024 Authors, Published BCREC Publishing Group. open access article under CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Language: Английский

Citations

0