Droplet-Based Microfluidics: Applications in Pharmaceuticals

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(7), P. 937 - 937

Published: June 28, 2023

Droplet-based microfluidics offer great opportunities for applications in various fields, such as diagnostics, food sciences, and drug discovery. A droplet provides an isolated environment performing a single reaction within microscale-volume sample, allowing fast with high sensitivity, throughput, low risk of cross-contamination. Owing to several remarkable features, droplet-based microfluidic techniques have been intensively studied. In this review, we discuss the impact microfluidics, particularly focusing on screening development. addition, surveyed methods device fabrication generation/manipulation. We further highlight some promising studies covering synthesis delivery that were updated last 5 years. This review researchers quick guide includes most up-to-date relevant information latest scientific findings development pharmaceutical field.

Language: Английский

---

From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts

Biomicrofluidics, Journal Year: 2025, Volume and Issue: 19(2)

Published: March 1, 2025

Droplet microfluidics has emerged as a versatile and powerful tool for various analytical applications, including single-cell studies, synthetic biology, directed evolution, diagnostics. Initially, access to droplet was predominantly limited specialized technology labs. However, the landscape is shifting with increasing availability of commercialized manipulation technologies, thereby expanding its use non-specialized Although these commercial solutions offer robust platforms, their adaptability often constrained compared in-house developed devices. Consequently, both within industry academia, significant efforts are being made further enhance robustness automation droplet-based not only facilitate transfer non-expert laboratories but also reduce experimental failures. This Perspective article provides an overview recent advancements aimed at accessibility systems enabling complex manipulations. The discussion encompasses diverse aspects such generation, reagent addition, splitting, washing, incubation, sorting, dispensing. Moreover, alternative techniques like double emulsions hydrogel capsules, minimizing or eliminating need microfluidic operations by end user, explored. These developments foreseen integration intricate manipulations users in workflows, fostering broader faster adoption across scientific domains.

Language: Английский

---

Categorising hybrid material microfluidic devices

Frontiers in Lab on a Chip Technologies, Journal Year: 2024, Volume and Issue: 3

Published: May 13, 2024

Microfluidic devices are useful tools for a wide range of biomedical, industrial, and environmental applications. Hybrid microfluidic utilising more than two materials increasingly being used their capacity to produce unique structures perform novel functions. However, an analysis publications across the field shows that whilst hybrid have been reported, there remains no system classifying which could help future researchers in optimising material selection. To resolve this issue, we propose primarily as containing either structural, chemical, or electrical components. This is expanded upon developed into hierarchy, with combinations different primary components categorised secondary tertiary device groupings. classification approach it describes can be combined create devices.

Language: Английский

---

Impedance Characteristics of Microfluidic Channels and Integrated Coplanar Parallel Electrodes as Design Parameters for Whole-Channel Analysis in Organ-on-Chip Micro-Systems

Biosensors, Journal Year: 2024, Volume and Issue: 14(8), P. 374 - 374

Published: Aug. 1, 2024

Microfluidics have revolutionized cell culture by allowing for precise physical and chemical environmental control. Coupled with electrodes, microfluidic can be activated or its changes sensed in real-time. We used our previously developed reliable stable device growth monitoring to design, fabricate, characterize a whole-channel impedance-based sensor it systematically assess the electrical electrochemical influences of channel boundaries coupled varying electrode sizes, distances, coatings, coverage. Our investigation includes both theoretical experimental approaches investigate how design parameters insulating boundary conditions change impedance characteristics. examined system various solutions using frequency range 0.5 Hz 1 MHz modulation voltage 50 mV. The results show that is directly proportional distance inversely coating, area, size. also demonstrate spacing dominant factor contributing impedance. In end, we summarize all relationships found comment on appropriateness this barrier cells blood vessel models organ-on-a-chip devices. This fundamental study help careful constructs require geometries biosensing.

Language: Английский

---

Intelligent optoelectrowetting digital microfluidic system for real-time selective parallel manipulation of biological droplet arrays

Lab on a Chip, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Optoelectrowetting technology generates virtual electrodes to manipulate droplets by projecting optical patterns onto the photoconductive layer. This method avoids complex design of physical circuitry dielectricwetting chips, compensating for inability reconstruct electrode. However, current relies on operators manually position droplets, draw patterns, and preset droplet movement paths. It lacks real-time feedback information ability independent control, which can lead miscontrol contamination. paper presents a combination optoelectrowetting with deep learning algorithms, integrating software photoelectric detection platform, develops an intelligent control system. First, target algorithm identifies characteristics in automatically generate movement. Simultaneously, tracking outputs trajectories ID efficient arrays tracking. The results show that system fusion multiple parallel realize automatic arrangement storage disordered without any additional sensing devices. Additionally, through automated system, cell suspension be precisely cultured specified medium according experimental requirements, growth trend is consistent observed well plate, significantly enhancing experiment's flexibility accuracy. In this paper, we propose applicable manipulation discrete droplets. would play crucial role advancing applications digital microfluidic biomedicine other fields.

Language: Английский

---

Model-Based Feedback Control for an Automated Micro Liquid Dispensing System Based on Contacting Droplet Generation through Image Sensing

Micromachines, Journal Year: 2023, Volume and Issue: 14(10), P. 1938 - 1938

Published: Oct. 18, 2023

Over the past few decades, micro liquid dispensing technology has been widely used in biology, chemistry, material and environmental sciences due to its efficacy processing multiple samples. For practical applications, precise effective droplet generation is very important. Despite numerous methods, implementation of droplet-on-demand still faces challenges concerning system complexity, precision, cost, robustness. In this work, a novel on-demand contacting method incorporated with model-based feedback control an image unit as sensor was proposed. By studying identification using techniques, model formation simplified. Then implemented volumes dispensed samples sensing signals by tuning related parameters adaptively resist disturbances. The proposed integrated applied homebuilt automated droplets ranging from 20 nanoliter 200 nanoliter. experimental results demonstrated high degree accuracy precision. Additionally, system's utility evaluated analyzing mutations genes associated sensorineural hearing loss, verifying effectiveness.

Language: Английский

---