A Label-free Optical Biosensor-Based Point-of-Care Test for the Rapid Detection of Monkeypox Virus DOI
Mete Aslan, Elif Seymour, Howard Brickner

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 5, 2024

ABSTRACT Diagnostic approaches that combine the high sensitivity and specificity of laboratory-based digital detection with ease use affordability point-of-care (POC) technologies could revolutionize disease diagnostics. This is especially true in infectious diagnostics, where rapid accurate pathogen critical to curbing spread disease. We have pioneered an innovative label-free platform utilizes Interferometric Reflectance Imaging Sensor (IRIS) technology. IRIS leverages light interference from optically transparent thin film, eliminating need for complex optical resonances enhance signal by harnessing power averaging shot-noise-limited operation achieve virtually unlimited sensitivity. In our latest work, we further improved previous ‘Single-Particle’ (SP-IRIS) technology allowing construction signature target nanoparticles (whole virus) a single image. new platform, ‘Pixel-Diversity’ (PD-IRIS), eliminated z-scan acquisition, required SP-IRIS, time-consuming expensive process, made more applicable POC settings. Using PD-IRIS, quantitatively detected Monkeypox virus (MPXV), etiological agent (Mpox) infection. MPXV was captured anti-A29 monoclonal antibody (mAb 69-126-3) on Protein G spots sensor chips were at limit-of-detection (LOD) - 200 PFU/ml (∼3.3 attomolar). PD-IRIS superior ELISA (LOD 1800 PFU/mL) used as comparator. The demonstrated using Herpes simplex virus, type 1 (HSV-1), Cowpox (CPXV). work establishes effectiveness opens possibilities its advancement clinical diagnostics Mpox POC. Moreover, modular can be adapted multiplex pathogens which high-affinity ligands are available bind their surface antigens capture them surface.

Language: Английский

Optimizing drug discovery: Surface plasmon resonance techniques and their multifaceted applications DOI Creative Commons
Biswajeet Acharya, Amulyaratna Behera,

Suchismeeta Behera

et al.

Chemical Physics Impact, Journal Year: 2023, Volume and Issue: 8, P. 100414 - 100414

Published: Dec. 5, 2023

In recent years, researchers have made significant contributions to the field of Surface Plasmon Resonance sensors and their potential applications. SPR features include real-time monitoring biomolecular interactions, label-free detection, high sensitivity, ability analyze multiple samples at same time. These characteristics make biosensors a valuable tool in pharmaceutical industry for ensuring drug efficacy safety. Furthermore, reputation providing quantitative qualitative superior performance applications such as development, environmental monitoring, food safety, medical diagnostics, clinical diagnosis, biological investigations, biomolecule interactions. This review will go over advances how they can be used discovery development. We also look challenges opportunities using industry.

Language: Английский

Citations

44
Integration of microfluidic chips with biosensors DOI Creative Commons

Masindi Sekhwama,

Kelvin Mpofu,

Sivarasu Sudesh

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(9)

Published: Aug. 23, 2024

Abstract The potential threat posed by disease outbreaks to diagnostic instruments demands the development of more effective biosensor technologies counteract risks. Diseases like SARS-CoV-2, Ebola, malaria, cholera, and many have demonstrated beyond limits health care that new advancements are required for early detection diagnosis. rising number diseases has led an increasing demand biosensors quicker utilize in healthcare settings. A incorporated with microfluidic chips offers improved compared traditional or classical biosensors. Microfluidic improve performance allowing automation, mixing, separation, throughput processing, transport analytes desired reactors. a chip sensitivity, easy operation can use small volumes samples process results. effectiveness depends also on materials used its fabrication there which reviewed this work. This paper reviews advantages enhance biosensors, fabricate chips, electrodes into time shortening processing at point service. work not previously addressed other including, integration cell-imprinted polymers sensors delved future outlook.

Language: Английский

Citations

11
Microfluidic biosensors: revolutionizing detection in DNA analysis, cellular analysis, and pathogen detection DOI
Reza Didarian, Mehdi Tayybi Azar

Biomedical Microdevices, Journal Year: 2025, Volume and Issue: 27(1)

Published: Feb. 26, 2025

Language: Английский

Citations

1
Translational paradigm in nanodiagnostics applications of functionalized zinc oxide nanoforms interface for pathogenic virus diagnostics DOI

Abha Gupta,

Apoorv Kirti,

Rabindranath Sahu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 509, P. 161260 - 161260

Published: March 5, 2025

Language: Английский

Citations

1
Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection DOI Creative Commons
Lihui Yuwen, Shifeng Zhang, Jie Chao

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(8), P. 822 - 822

Published: Aug. 15, 2023

Virus-related infectious diseases are serious threats to humans, which makes virus detection of great importance. Traditional virus-detection methods usually suffer from low sensitivity and specificity, time-consuming, have a high cost, etc. Recently, DNA biosensors based on nanotechnology shown potential in detection. nanotechnology, specifically tiles aptamers, has achieved atomic precision nanostructure construction. Exploiting the programmable nature nanostructures, researchers developed nanobiosensors that outperform traditional methods. This paper reviews history it briefly describes Baltimore classification virology. Moreover, advance by using is discussed detail compared with Finally, challenges faced summarized, perspective future development also provided.

Language: Английский

Citations

20
A Label-free Optical Biosensor-Based Point-of-Care Test for the Rapid Detection of Monkeypox Virus DOI Creative Commons
Mete Aslan, Elif Seymour, Howard Brickner

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 269, P. 116932 - 116932

Published: Nov. 14, 2024

Diagnostic approaches that combine the high sensitivity and specificity of laboratory-based digital detection with ease use affordability point-of-care (POC) technologies could revolutionize disease diagnostics. This is especially true in infectious diagnostics, where rapid accurate pathogen critical to curbing spread disease. We have pioneered an innovative label-free platform utilizes Interferometric Reflectance Imaging Sensor (IRIS) technology. IRIS leverages light interference from optically transparent thin film, eliminating need for complex optical resonances enhance signal by harnessing power averaging shot-noise-limited operation In our latest work, we further improved previous 'Single-Particle' (SP-IRIS) technology allowing construction signature target nanoparticles (whole virus) a single image. new platform, 'Pixel-Diversity' (PD-IRIS), eliminated z-scan acquisition, required SP-IRIS, time-consuming expensive process, made more applicable POC settings. Using PD-IRIS, quantitatively detected Monkeypox virus (MPXV), etiological agent (Mpox) infection. MPXV was captured anti-A29 monoclonal antibody (mAb 69-126-3) on Protein G spots sensor chips were at limit-of-detection (LOD) - 200 PFU/mL (∼3.3 aM). PD-IRIS superior ELISA (LOD 1800 PFU/mL) used as comparator. The demonstrated using Herpes simplex virus, type 1 (HSV-1), Cowpox (CPXV). work establishes effectiveness opens possibilities its advancement clinical diagnostics Mpox POC. Moreover, modular can be adapted multiplex pathogens which high-affinity ligands are available bind their surface antigens capture them surface.

Language: Английский

Citations

6
A comprehensive review of Interferometric Reflectance Imaging Sensor as a sensitive detection platform and its application areas DOI Creative Commons
Monireh Bakhshpour, Neşe Lortlar Ünlü, Elif Seymour

et al.

Biosensors and Bioelectronics X, Journal Year: 2025, Volume and Issue: unknown, P. 100574 - 100574

Published: Jan. 1, 2025

Language: Английский

Citations

0
A Label-free Optical Biosensor-Based Point-of-Care Test for the Rapid Detection of Monkeypox Virus DOI
Mete Aslan, Elif Seymour, Howard Brickner

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 5, 2024

ABSTRACT Diagnostic approaches that combine the high sensitivity and specificity of laboratory-based digital detection with ease use affordability point-of-care (POC) technologies could revolutionize disease diagnostics. This is especially true in infectious diagnostics, where rapid accurate pathogen critical to curbing spread disease. We have pioneered an innovative label-free platform utilizes Interferometric Reflectance Imaging Sensor (IRIS) technology. IRIS leverages light interference from optically transparent thin film, eliminating need for complex optical resonances enhance signal by harnessing power averaging shot-noise-limited operation achieve virtually unlimited sensitivity. In our latest work, we further improved previous ‘Single-Particle’ (SP-IRIS) technology allowing construction signature target nanoparticles (whole virus) a single image. new platform, ‘Pixel-Diversity’ (PD-IRIS), eliminated z-scan acquisition, required SP-IRIS, time-consuming expensive process, made more applicable POC settings. Using PD-IRIS, quantitatively detected Monkeypox virus (MPXV), etiological agent (Mpox) infection. MPXV was captured anti-A29 monoclonal antibody (mAb 69-126-3) on Protein G spots sensor chips were at limit-of-detection (LOD) - 200 PFU/ml (∼3.3 attomolar). PD-IRIS superior ELISA (LOD 1800 PFU/mL) used as comparator. The demonstrated using Herpes simplex virus, type 1 (HSV-1), Cowpox (CPXV). work establishes effectiveness opens possibilities its advancement clinical diagnostics Mpox POC. Moreover, modular can be adapted multiplex pathogens which high-affinity ligands are available bind their surface antigens capture them surface.

Language: Английский

Citations

3