Development of an enhanced liver scaffold recellularization using fibronectin DOI
Sadia Afrin, Usha Yadav,

Chandra Jit Yadav

et al.

Journal of Biomaterials Applications, Journal Year: 2025, Volume and Issue: unknown

Published: June 5, 2025

Decellularized liver scaffolds offer a promising foundation for tissue engineering and regenerative medicine. However, several challenges such as poor cell adhesion, inefficient reseeding, inadequate vascularization, high risk of blood clot formation continue to hinder their clinical application. While fibronectin (FN) has been widely used enhance scaffold functionality, its potential liver-specific applications remains largely unexplored. In this study, we developed perfusion-assisted FN coating technique improve the adhesion endothelial cells (EA.hy926) hepatocytes (HepG2), thereby enhancing overall biocompatibility scaffolds. was carefully introduced into decellularized rat scaffolds, allowing targeted deposition across both vascular parenchymal compartments optimize cellular attachment. Following portal vein reseeding 7 days bioreactor incubation, FN-coated showed significantly better within vessel structures increased HepG2 coverage throughout tissue. Immunohistochemistry (IHC) confirmed enhanced proliferation, while TUNEL RT-qPCR analyses indicated improved viability functionality. Additionally, ex vivo perfusion tests demonstrated reduced thrombogenicity, likely due endothelialization lower platelet adhesion. These findings highlight functionalization an effective bioengineering approach overcoming key barriers in biocompatibility, integration By extending known benefits beyond previously studied kidney heart research introduces strategy advancing bioengineered grafts transplantation models.

Language: Английский

Toward Intelligent Materials with the Promise of Self-Healing Hydrogels in Flexible Devices DOI Open Access

Han-Seop Song,

Md. Mahamudul Hasan Rumon, Mohammad Mizanur Rahman Khan

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(4), P. 542 - 542

Published: Feb. 19, 2025

Flexible sensors are revolutionizing wearable and implantable devices, with conductive hydrogels emerging as key materials due to their biomimetic structure, biocompatibility, tunable transparency, stimuli-responsive electrical properties. However, fragility limited durability pose significant challenges for broader applications. Drawing inspiration from the self-healing capabilities of natural organisms like mussels, researchers embedding self-repair mechanisms into improve reliability lifespan. This review highlights recent advances in (SH) hydrogels, focusing on synthesis methods, healing mechanisms, strategies enhance multifunctionality. It also explores wide-ranging applications, including vivo signal monitoring, biochemical sensors, supercapacitors, flexible displays, triboelectric nanogenerators, bioelectronics. While progress has been made, remain balancing efficiency, mechanical strength, sensing performance. offers insights overcoming these obstacles discusses future research directions advancing SH hydrogel-based bioelectronics, aiming pave way durable, high-performance devices next-generation technologies.

Language: Английский

Citations

2
A Detailed Review of pH and its Applications DOI Open Access
Ritu Tiwari, Gaurav Sanjay Mahalpure

Journal of Pharmaceutical and Biopharmaceutical Research, Journal Year: 2025, Volume and Issue: 6(2), P. 492 - 505

Published: April 4, 2025

The pH of a solution is an important measure how acidic or alkaline it is. It shows the concentration hydrogen ions in through negative logarithm. This measurement gives insight into substance behaves chemically. Scientists use electrode to get accurate readings, which provides reliable measurements for different tests. key concept chemistry and relevant many scientific fields, including biochemistry, biology, physics, medicine, agriculture, environmental science. Keeping right level crucial industries, especially chemical manufacturing, food production, protection, as can directly affect quality lab results. Even small changes lead major differences accuracy analytical outcomes. particularly when following strict guidelines pharmacopoeias conducting research using databases like Scopus PubMed, where data vital. article explains essential aspects needed effectively analysis. detailed look at other factors necessary practical testing. Additionally, highlights various ways are used, showing their importance across industrial areas.

Language: Английский

Citations

0
Evolution of Preclinical Models for Glioblastoma Modelling and Drug Screening DOI Creative Commons
Grace Thomas, Ruman Rahman

Current Oncology Reports, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Language: Английский

Citations

0
Enhancing detection and monitoring of circulating tumor cells: Integrative approaches in liquid biopsy advances DOI Creative Commons

Thanmayi Velpula,

Buddolla Viswanath

The Journal of Liquid Biopsy, Journal Year: 2025, Volume and Issue: 8, P. 100297 - 100297

Published: April 29, 2025

Liquid biopsy offers a minimally invasive method for detecting and monitoring cancer, with key biomarkers including circulating tumor cells (CTCs), cell-free DNA (cfDNA), extracellular vesicles (EVs). Among these, CTCs provide dynamic insights into heterogeneity, metastatic potential, therapeutic resistance through molecular profiling single-cell analysis. This review examines recent advancements in technologies such as microfluidics, nanotechnology, next-generation sequencing (NGS), which have improved the accuracy clinical use of CTC detection. A comparative analysis liquid techniques highlights strengths limitations platforms, NGS, digital PCR (ddPCR), quantitative (qPCR), providing diagnostic significance. Combining genomic, transcriptomic, proteomic analyses artificial intelligence (AI) tools has enhanced supports personalized treatment decisions precision oncology. Despite notable progress, issues like assay standardization, sample variability, regulatory complexity, data integration still limit widespread use. Future directions emphasize interdisciplinary innovation, validation, robust bioinformatics frameworks to facilitate seamless incorporation CTC-based standard oncology practice. Overcoming these challenges may allow become tool early cancer detection, ongoing monitoring, individualized treatment.

Language: Английский

Citations

0
Advancing Organ-on-a-Chip Systems: The Role of Scaffold Materials and Coatings in Engineering Cell Microenvironment DOI Open Access

Guido Andrés Ramírez-González,

Chiara Consumi-Tubito,

Ernesto Vargas-Méndez

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(9), P. 1263 - 1263

Published: May 6, 2025

For organ-on-a-chip (OoC) engineering, the use of biocompatible coatings and materials is not only recommended but essential. Extracellular matrix (ECM) components are commonly used as due to their effects on cell orientation, protein expression, differentiation, adhesion. Among most frequently collagen, fibronectin, Matrigel, according specific type intended OoC application. Additionally, such polydimethylsiloxane (PDMS), thermoplastics, chitosan, alginate serve scaffolding biomechanical properties biocompatibility. Here, we discuss some employed coating techniques, including SAMs, dip coating, spin microcontact printing, 3D bioprinting, each offering advantages drawbacks. Current challenges comprise enhancing biocompatibility, exploring novel materials, improving scalability reproducibility.

Language: Английский

Citations

0
Bridging the Gap: How Organ‐on‐a‐Chip Technology Facilitates the Battle against Glioma DOI Creative Commons
Su Liu, Zhenyu Gong,

Dairan Zhou

et al.

Small Science, Journal Year: 2025, Volume and Issue: unknown

Published: June 3, 2025

Glioma, a highly aggressive brain tumor, presents significant challenges in understanding its complex pathophysiology and developing effective treatments. This review critically examines the current glioma modeling platforms, including 2D culture, 3D cultures, glioma‐on‐a‐chip (GoC) models, animal with focus on their applications recapitulating tumor microenvironment evaluating treatment effectiveness. Particular attention is given to microfluidic GoC which offer unique capabilities mimic pathophysiological complexity of glioblastoma, interactions blood–brain barrier (BBB) vascularization. Recent advancements these models are explored, highlighting potential for clinical translation through improved drug permeability central nervous system, progress, therapeutic responses. In end, discusses perspective establishes key criteria designing robust clinically relevant models. By addressing future opportunities, critical role platforms bridging gap between preclinical research outcomes emphasized, offering promising approach more personalized therapies.

Language: Английский

Citations

0
Development of an enhanced liver scaffold recellularization using fibronectin DOI
Sadia Afrin, Usha Yadav,

Chandra Jit Yadav

et al.

Journal of Biomaterials Applications, Journal Year: 2025, Volume and Issue: unknown

Published: June 5, 2025

Decellularized liver scaffolds offer a promising foundation for tissue engineering and regenerative medicine. However, several challenges such as poor cell adhesion, inefficient reseeding, inadequate vascularization, high risk of blood clot formation continue to hinder their clinical application. While fibronectin (FN) has been widely used enhance scaffold functionality, its potential liver-specific applications remains largely unexplored. In this study, we developed perfusion-assisted FN coating technique improve the adhesion endothelial cells (EA.hy926) hepatocytes (HepG2), thereby enhancing overall biocompatibility scaffolds. was carefully introduced into decellularized rat scaffolds, allowing targeted deposition across both vascular parenchymal compartments optimize cellular attachment. Following portal vein reseeding 7 days bioreactor incubation, FN-coated showed significantly better within vessel structures increased HepG2 coverage throughout tissue. Immunohistochemistry (IHC) confirmed enhanced proliferation, while TUNEL RT-qPCR analyses indicated improved viability functionality. Additionally, ex vivo perfusion tests demonstrated reduced thrombogenicity, likely due endothelialization lower platelet adhesion. These findings highlight functionalization an effective bioengineering approach overcoming key barriers in biocompatibility, integration By extending known benefits beyond previously studied kidney heart research introduces strategy advancing bioengineered grafts transplantation models.

Language: Английский

Citations

0